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Molecular Cloning and Expression Analysis of a Terpene Synthase Gene, HcTPS2, in Hedychium coronarium

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Abstract

The Hedychium coronarium can emit a strong scent which is mainly composed of monoterpenes. A cDNA clone, HcTPS2 (H. coronarium terpene synthases), was cloned from H. coronarium flower. The gene has an open reading frame of 1,788 bp which encodes a protein of 596 amino acids with a calculated molecular mass of 66.7 kDa. The deduced amino acid sequence shows 35–38% identity with known monoterpene synthases in other angiosperm species. HcTPS2 was appreciably expressed in the petals, sepals, and stamens of H. coronarium, whereas no expression signal was detected in those of nonscented species. To the best of our knowledge, this is for the first time to clone the terpene synthase gene from H. coronarium, which provides the basis for biotechnological manipulation of scent composition in H. coronarium.

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Abbreviations

HcTPS2:

Hedychium coronarium terpene synthases 2

RACE:

Rapid amplification of cDNAs ends

RT-PCR:

Reverse transcription PCR

SDS:

Sodium dodecyl sulfate

SSC:

Sodium chloride/sodium citrate (buffer)

TPS:

Terpene synthase

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Acknowledgments

This work was supported by the project of National Natural Science Foundation of China (30972026) and Natural Science Foundation of Guangdong Province (07006667) to Y. F.

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  1. College of Horticulture, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Ruihong Li & Yanping Fan

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  1. Ruihong Li
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  2. Yanping Fan
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Correspondence to Yanping Fan.

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Li, R., Fan, Y. Molecular Cloning and Expression Analysis of a Terpene Synthase Gene, HcTPS2, in Hedychium coronarium . Plant Mol Biol Rep 29, 35–42 (2011). https://doi.org/10.1007/s11105-010-0205-1

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