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Transcriptional Profiles of Roots of Different Soybean Genotypes Subjected to Drought Stress

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Abstract

To identify differentially expressed genes in soybean grown under different drought conditions, cDNA libraries from roots of different genotypes were constructed. Genes of contrasting genotypes of soybean were found to be differentially expressed in plants exposed to drought conditions. A total of 753 no redundant clones were identified by PCR, and these were printed on microarray glass slides. Probes of total RNA were prepared from bulked roots subjected to 25 and 50 min (Bulk 1) or 75 and 100 min (Bulk 2) of drought stress. Differential expression of 145 genes, involved in metabolic pathways responsive to biotic and abiotic stresses, was observed. These genes were classified into nine functional categories, including energy, transcription factors, metabolism, stress response, protein synthesis, cell communication, cell cycle, cell transport, and unknown function. The functionality of some of these genes was confirmed by quantitative real-time PCR (qRT-PCR).

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Acknowledgments

The authors thank the financial support from CAPES, JIRCAS, and Embrapa Soybean.

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Authors and Affiliations

  1. Paulist State University Via de Acesso Prof. Paulo Donato Castellane s/n, Posted code 14884-900, Jaboticabal, SP, Brazil

    Renata Stolf-Moreira, Eliana G. M. Lemos, Lúcia Carareto-Alves, Jackson Marcondes & Rodrigo M. Pereira

  2. Department of General Biology, Londrina State University, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, Cx. Postal 6001, Posted code 86051-990, Londrina, PR, Brazil

    Selma S. Pereira & Amanda A. P. Rolla

  3. Embrapa Soybean, Rodovia Carlos João Strass, Distrito de Warta, Cx. Postal 231, Posted code 86001-970, Londrina, PR, Brazil

    Norman Neumaier, Eliseu Binneck, Ricardo V. Abdelnoor, Maria C. N. de Oliveira, Francismar C. Marcelino, José R. B. Farias & Alexandre L. Nepomuceno

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  1. Renata Stolf-Moreira
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  2. Eliana G. M. Lemos
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  3. Lúcia Carareto-Alves
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  14. Alexandre L. Nepomuceno
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Correspondence to Renata Stolf-Moreira.

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Stolf-Moreira, R., Lemos, E.G.M., Carareto-Alves, L. et al. Transcriptional Profiles of Roots of Different Soybean Genotypes Subjected to Drought Stress. Plant Mol Biol Rep 29, 19–34 (2011). https://doi.org/10.1007/s11105-010-0203-3

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  • DOI: https://doi.org/10.1007/s11105-010-0203-3

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