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A B Functional Gene Cloned from Lily Encodes an Ortholog of Arabidopsis PISTILLATA (PI)

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Abstract

The classical ABC model proposed for flower development in Arabidopsis and Antirrhinum appropriately sheds light on the biological process of flower development and differentiation and serves in manipulating the floral structure of other important ornamental plants. In this study, LLGLO1, a B functional gene from Lilium longiflorum was isolated and characterized. RT-PCR analysis elucidated that temporal and spatial expression pattern of LLGLO1. This putative gene was strongly expressed in 1, 2, and 3 whorl organs, i.e., outer whorl tepals, inner whorl tepals, and stamens. Genetic effect of LLGLO1 was assayed by ectopic expression in model plant Arabidopsis. Transformed plants showed homeotic transformation of sepals into petaloid sepals in the first whorl, which is similar to the transgenic plants of 35S::PI. So LLGLO1 was one member of GLO/PI sub-family gene to function in flower development.

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Abbreviations

PI :

PISTILLATA

GLO :

GLOBOSA

AP2 :

APETALA2

AP3 :

APETALA3

DEF :

DEFICIENS

RACE:

rapid amplification of cDNA ends

RNAi:

RNA interference

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Acknowledgments

We thank two anonymous reviewers for their comments and suggestions, which have been useful in improving presentation of this article. We thank Prof. Zewei Luo for constructive comments on the manuscript. We also thank Dr. Xinchun Lin and Dr. Fang Xie for helping to grow Arabidopsis and transform gene. This study was supported by the National Natural Science Foundation of China (30700444, 30972407), Jiangsu Provincial Key Basic Research foundation for Universities(07KJA21019), and the Shanghai Science and Technology Committee (07ZR14008).

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Authors and Affiliations

  1. Laboratory of Population and Quantitative Genetics, the State Key Laboratory of Genetic Engineering, Institute of Biostatistics, School of Life Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Xiaoping Wu, Zewei Luo & Xiaohua Hu

  2. The Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Xiaoping Wu, Jisen Shi & Mengli Xi

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  1. Xiaoping Wu
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  2. Jisen Shi
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  3. Mengli Xi
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  4. Zewei Luo
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  5. Xiaohua Hu
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Correspondence to Jisen Shi or Xiaohua Hu.

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Wu, X., Shi, J., Xi, M. et al. A B Functional Gene Cloned from Lily Encodes an Ortholog of Arabidopsis PISTILLATA (PI). Plant Mol Biol Rep 28, 684–691 (2010). https://doi.org/10.1007/s11105-010-0193-1

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