Abstract
To elucidate the genetic and molecular mechanisms of plant architecture, a Cucumber Lateral Suppressor gene (CLS) for the lateral branching development in cucumber branching line S06 was cloned and characterized. Full-length cDNA of CLS (EU503217) was isolated by performing rapid amplification of cDNA ends polymerase chain reaction. Phylogenetic analysis showed that the gene CLS is closely related to Lateral Suppressor that encodes transcriptional regulator proteins belonging to GRAS family of known transcription factor. Southern blot analysis revealed that CLS gene has one copy in cucumber genome. The expression patterns revealed that transcripts were detected in all organs, but not in internodes. In addition, the transcript level in male flowers is higher than the female flowers and fruit. Introduction of the Pro35s:CLS overexpression vector into Arabidopsis las plants led to a full complementation of the mutant phenotypes. Moreover, RNA in situ hybridization showed that the pattern of CLS transcript was accumulated in the leaf axils in different developmental stage, which was similar to LAS in Arabidopsis. These results together indicate that CLS may play an important role in cucumber axillary meristem initiation and thus the lateral branch formation.
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Abbreviations
- CLS :
-
Cucumber Lateral Suppressor
- SAM:
-
Shoot apical meristem
- RACE:
-
Rapid amplification of cDNA ends
- UTR:
-
Untranslated region
- R1–R3:
-
rescured plants
- NR:
-
not rescured plant
- C:
-
Columbia wild type
- L:
-
las mutant
- CK:
-
H2O Control
- MF:
-
male flower
- FF:
-
female flower
- SN:
-
internode
- ST:
-
shoot tip
- LF:
-
leaf
- FR:
-
fruit
- RT:
-
root
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Acknowledgments
The authors thank Klaus Theres, Max Planck Institute for Plant Breeding Research, Germany for providing las mutant. We are grateful to Hai Huang and Da Luo, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, for their respective technical assistances. This work was supported by National “863” Project (Number 2008AA10Z150) and Shanghai Leading Academic Discipline Project (Number B209).
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Yuan, LH., Pan, JS., Wang, G. et al. The Cucumber Lateral Suppressor Gene (CLS) Is Functionally Associated with Axillary Meristem Initiation. Plant Mol Biol Rep 28, 421–429 (2010). https://doi.org/10.1007/s11105-009-0164-6
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DOI: https://doi.org/10.1007/s11105-009-0164-6