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Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.)

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A pathogen-inducible ethylene-responsive factor gene, EREB1, was isolated and characterized in cotton (Gossypium barbadense L.). The deduced amino acid sequence of EREB1 had some typical features of transcription factors including nuclear localization signals, an acidic activation domain, and a conserved DNA binding domain. As a single copy gene in the cotton genome, the level of expression of EREB1 in roots and leaves was significantly elevated following treatment with Verticillium wilt toxin. Presence of a GCC box-binding ability motif indicated that the EREB1 protein was capable of binding to GCC box elements in vitro as a transcriptional activator. In tobacco, overexpression of EREB1 activated pathogenesis-related genes under normal growth conditions. These results suggested that EREB1 might play an important role in biotic stress signal transduction pathways and that the overexpression of EREB1 might serve as a viable approach to enhance disease resistance t in cotton.

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Electrophoretic mobility shift assays


Ethylene-responsive factor


Nuclear localization signal




Salicylic acid


Jasmonic acid


Abscisic acid


  • Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M (1998) A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J 17:5484–5496

    Article  CAS  PubMed  Google Scholar 

  • Ausubel FM, Brent R, Kingston RE, Moore D, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Wiley, New York

    Google Scholar 

  • Broekaert WF, Delaure SL, De Bolle MF, Cammue BP (2006) The role of ethylene in host–pathogen interactions. Annu Rev Phytopathol 44:393–416

    Article  CAS  PubMed  Google Scholar 

  • Chen W, Provart NJ, Glazebrook J, Katagiri F, Wang X, Zhu T (2002) Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. Plant Cell 14:559–574

    Article  CAS  PubMed  Google Scholar 

  • Daayf F, Nicole M, Boher B, Pando A, Geiger JP (1997) Early vascular defense reactions of cotton roots infected with a defoliating mutant strain of Verticillium dahliae. Eur J Plant Pathol 103:125–136

    Article  CAS  Google Scholar 

  • Dubery IA, Slater V (1997) Induced defence responses in cotton leaf disks by elicitors from Verticillium dahliae. Phytochemistry 44(8):1429–1434

    Article  CAS  Google Scholar 

  • Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M (2000) Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression. Plant Cell 12:393–404

    Article  CAS  PubMed  Google Scholar 

  • Gu YQ, Yang C, Thara VK, Zhou J, Martin GB (2000) Tomato transcription factor Pti4, Pti5, and Pti6 activate defence responses when expressed in Arabidopsis. Plant Cell 12:771–785

    Article  CAS  PubMed  Google Scholar 

  • Guo H, Ecker JR (2004) The ethylene signaling pathway: new insights. Curr Opin Plant Biol 7(1):40–49

    Article  CAS  PubMed  Google Scholar 

  • Kizis D, Pages M (2002) Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through the drought-responsive element in an ABA-dependent pathway. Plant J 30(6):679–689

    Article  CAS  PubMed  Google Scholar 

  • Lee JH, Kim DM, Lee JH, Kim J, Bang JW, Kim WB, Pai HS (2005) Functional characterization of NtCEF1, an AP2/EREBP-type transcriptional activator highly expressed in tobacco callus. Planta 222(2):211–224

    Article  CAS  PubMed  Google Scholar 

  • Lorenzo O, Piqueras R, Sanchez-Serrano JJ, Solano R (2003) Ethylene response factor1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15(1):165–178

    Article  CAS  PubMed  Google Scholar 

  • Ma C, Jian GL, Sun WJ (1997) China cotton breeding resistance to Verticillium wilt status, problems and discussion. Sci Agri Sinica (China) 30:58–64

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497

    Article  CAS  Google Scholar 

  • Nishiui T, Suzuki K, Kitajima S, Sato H, Shinshi H (2002) Wounding activates immediate early transcription of genes for ERFs in tobacco plants. Plant Mol Biol 49(5):473–482

    Article  Google Scholar 

  • Ohme-Takagi M, Shinshi H (1995) Ethylene inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 7(2):173–182

    Article  CAS  PubMed  Google Scholar 

  • Ohta M, Ohme-Takagi M, Shinshi H (2000) Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions. Plant J 22(1):29–38

    Article  CAS  PubMed  Google Scholar 

  • Oñate-Sánchez L, Anderson JP, Young J, Singh KB (2007) AtERF14, a member of the ERF family of transcription factors, plays a non reduction role in plant defense. Plant Physiol 43(1):400–409

    Google Scholar 

  • Park JM, Park CJ, Lee SB, Ham BK, Shin R, Peak KH (2001) Over expression of the tobacco Tsi1 gene encoding anEREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. Plant Cell 13(5):1035–1046

    Article  CAS  PubMed  Google Scholar 

  • Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration and cold inducible gene expression. Biochem Biophys Res Commun 290:998–1009

    Article  CAS  PubMed  Google Scholar 

  • Sessa G, Meller Y, Fluhr R (1995) A GCC element and a G-box motif participate in ethylene-induced expression of the PRB-1b gene. Plant Mol Biol 28(1):145–153

    Article  CAS  PubMed  Google Scholar 

  • Shi YH, Zhu SW, Mao XZ, Feng JX, Qin YM, Zhang L, Cheng J, Wei LP, Wang ZY, Zhu YX (2006) Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell 18(3):651–664

    Article  CAS  PubMed  Google Scholar 

  • Shin R, Park JM, An JM, Peak KH (2002) Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens. Mol Plant Microbe Interact 15(10):983–989

    Article  CAS  PubMed  Google Scholar 

  • Solano R, Stepanova A, Chao Q, Rcker JR (1998) Nuclear event in ethylene signaling: a transcriptional cascade mediated by ethylene-insensitive and ethylene-response-factor. Genes Dev 12(23):3703–3714

    Article  CAS  PubMed  Google Scholar 

  • Tournier B, Sanchez-Ballesta MT, Jones B, Pesquet E, Regad F, Latche A, Pech JC, Bouzayen M (2003) New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element. FEBS Lett 550:149–154

    Article  CAS  PubMed  Google Scholar 

  • Xu ZS, Xia LQ, Chen M, Cheng XG, Zhang RZ, Ma YZ (2007) Isolation and molecular characterization of the Triticum aestivum L ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance. Plant Mol Biol 65:719–732

    Article  CAS  PubMed  Google Scholar 

  • Yap YK, Kodama Y, Waller F, Chung KM, Ueda H, Nakamura K, Oldsen M, Yoda H, Yamaguchi Y, Sano H (2005) Activation of a novel transcription factor through phosphorylation by WIPK, a wound-induced mitogen-activated protein kinase in tobacco plants. Plant Physiol 139:127–137

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Zhang Z, Chen J, Chen Q, Wang X, Huang R (2005) Expressing TERF1 in tobacco enhances drought tolerance and abscisic acid sensitivity during seedling development. Planta 22(3):94–501

    Google Scholar 

  • Zhang Z, Yao W, Dong N, Liang H, Liu H, Huang R (2007) A novel ERF transcription activator in wheat and its induction kinetics after pathogen and hormone treatments. J Exp Bot 58(11):2993–3003

    Article  CAS  PubMed  Google Scholar 

  • Zhou J, Tang X, Martin GB (1997) The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J 16(11):3207–3218

    Article  CAS  PubMed  Google Scholar 

  • Zhu HQ et al (1999) Study on pathogenic type in Anyang strains of Verticillium dahliae Kleb. Acta Gossypii Sinica 11(6):312–317

    Google Scholar 

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This research was financially supported by the National “863” High-tech Project (2006011001042). We are grateful to Dr. YZ Ma and Dr. ZS Xu (Institute of Crop Science, Chinese Academy of Agricultural Sciences) for providing the prokaryotic expression vector and Dr. M Lin and Dr. ZX Peng (Institute of Biotechnology Research Chinese Academy of Agricultural Sciences) for their helpful advice in the experiments.

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Correspondence to Fuguang Li.

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Meng, X., Li, F., Liu, C. et al. Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Plant Mol Biol Rep 28, 176–183 (2010).

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