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Isolation and Characterization of Putative Acetyl-CoA Carboxylases in Arachis hypogaea L.

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Abstract

Candidate genes of multisubunit (MS) and multifunctional (MF) acetyl-CoA carboxylase (ACCase) including accB1, accB2, accC, accA, accD, and MF-ACCase were isolated from peanut by sequencing a full-length cDNA library and homology-based cloning. Primary structures of peanut ACCases were highly conserved, especially biochemical function domains, compared with other higher plants and Escherichia coli. The numbers of homologs of each ACCase gene expressed in developing seed of peanut varied from two to five. Two editing sites of peanut accD were identified by comparing genomic DNA and its corresponding cDNAs. The MS-ACCase of Arachis hypogaea and Arabidopsis thaliana shared a conservative structure of gene organization. Semiquantitative reverse transcription polymerase chain reaction analysis indicated that MS-ACCase and MF-ACCase genes were expressed in all peanut tissues examined, but their mRNA expression level were quite different. In peanut seed development, accC, accA, accD, and MF-ACCase mRNAs were expressed strongly at 60 days after pegging.

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Abbreviations

ACCase:

acetyl-CoA carboxylase

MS:

multisubunit

MF:

multifunctional

BCCP:

biotin carboxyl carrier protein

BC:

biotin carboxylase

CT:

carboxyltransferase

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Acknowledgement

This work was supported by grants from the National High Technology Research and Development Program of the People’s Republic of China (2006AA10A114), Shandong Academy of Agricultural Sciences Foundation (2006YCX030 and 2007YCX001), and Postdoctoral Foundation of Shandong Province (200701004).

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  1. High-Tech Research Center, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Meng-Jun Li, Han Xia, Chuan-Zhi Zhao, Ai-Qin Li, Chang-Sheng Li, Yu-Ping Bi, Shu-Bo Wan & Xing-Jun Wang

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  1. Meng-Jun Li
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Correspondence to Xing-Jun Wang.

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Li, MJ., Xia, H., Zhao, CZ. et al. Isolation and Characterization of Putative Acetyl-CoA Carboxylases in Arachis hypogaea L.. Plant Mol Biol Rep 28, 58–68 (2010). https://doi.org/10.1007/s11105-009-0126-z

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