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Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

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An Erratum to this article was published on 10 February 2010

An Erratum to this article was published on 10 February 2010

Abstract

Cyclamen persicum Mill. is a widely grown ornamental species that is clonally propagated by somatic embryogenesis. To better understand the biology of somatic embryo development in C. persicum, detailed proteomic (two-dimensional gel electrophoresis) and mass spectrometric analyses of somatic embryos at globular, torpedo, and germinating stages of development, along with nonembryogenic callus and zygotic embryos, were conducted. Of ~460 proteins resolved in two-dimensional gels, 35 proteins were differentially expressed and could be reproducibly displayed across an isoelectric focusing range of 5 to 8. Among those proteins, five were constitutively expressed, 13 were upregulated, nine were downregulated, and eight were deemed as novel proteins during the torpedo stage. A total of 35 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and only four proteins were identified and these were available in public protein databases. The remaining protein spots were subsequently analyzed by MALDI-TOF-TOF-MS, and six proteins were then identified. These findings suggested that specific proteins are involved in the regulation of somatic embryogenesis.

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References

  • Aleith F, Richter G (1990) Gene expression during induction of somatic embryogenesis in carrot cell suspensions. Planta 183:17–24

    Google Scholar 

  • Amsterdam A, Pitzer F, Baumeister W (1993) Changes in intracellular localization of proteasomes in immortalized ovarian granulosa cells during mitosis associated with a role in cell cycle control. Proc Natl Acad Sci U S A 90:99–103. doi:10.1073/pnas.90.1.99

    Article  CAS  PubMed  Google Scholar 

  • Baumberger N, Ringli C, Keller B (2001) The chimeric leucine2rich repeat/extensin cell wall protein LRX1 is required for root hair morphogenesis in Arabidopsis thaliana. Genes Dev 15:1128–1139. doi:10.1101/gad.200201

    Article  CAS  PubMed  Google Scholar 

  • Bian FH, You CR, Gong XQ, Qu FN (2008) Induction of embryogenic callus and somatic embryogenesis from the tubers of seedlings in Cyclamen persicum Mill. Journal of Yantai University 21:281–285

    CAS  Google Scholar 

  • Blanco M, Nieves N, Sánchez M, Borroto C, Castillo R, González J, Escalona M, Báez E, Hernández Z (1997) Protein changes associated with plant regeneration in embryogenic calli of sugarcane (Saccharum sp.). Plant Cell Tissue Organ Cult 51:153–158. doi:10.1023/A:1005963925773

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3

    Article  CAS  PubMed  Google Scholar 

  • Chin HL, Ralph SC (1980) Glyceraldehyde-3-phosphate dehydrogenase in greening Zea mays L. leaves. Plant Physiol 65:897–901. doi:10.1104/pp.65.5.897

    Article  Google Scholar 

  • Choi JH, Sung ZR (1984) Two-dimensional gel analysis of carrot somatic embryonic proteins. Plant Mol Biol Rep 2:19–25. doi:10.1007/BF02885643

    Article  CAS  Google Scholar 

  • Chugh A, Khurana P (2002) Gene expression during somatic embryogenesis: recent advances. Curr Sci 83:715–730

    CAS  Google Scholar 

  • Dodeman VL, Ducreux G (1996) Total protein pattern expression during induction and development of carrot somatic embryos. Plant Sci 120:57–69. doi:10.1016/S0168-9452(96)04487-1

    Article  CAS  Google Scholar 

  • Fehér A, Pasternak TP, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue Organ Cult 74:201–228. doi:10.1023/A:1024033216561

    Article  Google Scholar 

  • Giroux R, Pauls KP (1996) Characterization of embryogenesis-related proteins in alfalfa. Physiol Plant 96:585–592. doi:10.1111/j.1399-3054.1996.tb00230.x

    Article  CAS  Google Scholar 

  • Giroux RW, Pauls KP (1997) Characterization of somatic embryogenesis-related cDNAs from alfalfa (Medicago sativa L.). Plant Mol Biol 33:393–404. doi:0.1023/A:1005786826672

    Article  CAS  PubMed  Google Scholar 

  • Hawkes HY, Wainwright H (1987) In vitro organogenesis of Cyclamen persicum Mill. seedling tissue. Acta Hortic 212:711–714

    Google Scholar 

  • Ito H, Iwabuchi M, Ogawa K (2003) The sugar-metabolic enzymes aldolase and triose-phosphate isomerase are targets of glutathionylation in Arabidopsis thaliana: detection using biotinylated glutathione. Plant Cell Physiol 44:655–660. doi:10.1093/pcp/pcg098

    Article  CAS  PubMed  Google Scholar 

  • Kiviharju E, Tuominen U, Tormala T (1992) The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill. Plant Cell Tissue Organ Cult 28:187–194. doi:10.1007/BF00055516

    Article  Google Scholar 

  • Kreuger M, Postma E, Brouwer Y, van Holst GJ (1995) Somatic embryogenesis of Cyclamen persicum in liquid medium. Physiol Plant 94:605–612. doi:10.1111/j.1399-3054.1995.tb00974.x

    Article  CAS  Google Scholar 

  • Lippert D, Zhuang J, Ralph S, Ellis DE, Gilbert M, Olafson R, Ritland K, Ellis B, Carl J (2005) Proteome analysis of early somatic embryogenesis in Picea glauca. Proteomics 5:461–473. doi:10.1002/pmic.200400986

    Article  CAS  PubMed  Google Scholar 

  • Milena M, Bracale M, Espen L, Prinsi B, Negri AS, Vannini C (2008) Proteomic analysis of somatic embryogenesis in Vitis vinifera. Plant Cell Rep 27:347–356. doi:10.1007/s00299-007-0438-0

    Article  Google Scholar 

  • Mordhorst AP, Toonen MAJ, de Vries SC (1997) Plant embryogenesis. Crit Rev Plant Sci 16:535–576. doi:10.1080/713608156

    Article  Google Scholar 

  • Motoyasu O, Takiko S (1991) Somatic embryogenesis and plant regeneration from Cyclamen persicum Mill. leaf cultures. Plant Tissue Culture Letters 8:121–123

    Google Scholar 

  • Nogueira FCS, Goncalves EF, Jereissati ES, Santos M, Costa JH, Oliveira-Neto OB, Soares AA, Domont GB, Campos FAP (2007) Proteome analysis of embryogenic cell suspensions of cowpea (Vigna unguiculata). Plant Cell Rep 26:1333–1343. doi:10.1007/s00299-007-0327-6

    Article  CAS  PubMed  Google Scholar 

  • Pardo M, Ward M, Pitarch A, Sánchez M, Nombela C, Blackstock W, Gil C (2000) Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Electrophoresis 21:2651–2659. doi:10.1002/1522-2683(20000701)21:13<2651::AID-ELPS2651>3.0.CO;2-3

    Article  CAS  PubMed  Google Scholar 

  • Ruffoni B, Semeria L, Profumo P (2000) Cyclamen persicum Mill. somatic embryos developed in suspension cultures: histological analysis and conversion to plant. Acta Hortic 520:83–90

    Google Scholar 

  • Schmidt TH, Ewald A, Seyring M, Hohe A (2006) Comparative analysis of cell cycle events in zygotic and somatic embryos of Cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds. Plant Cell Rep 25:643–650. doi:10.1007/s00299-006-0130-9

    Article  CAS  PubMed  Google Scholar 

  • Schwenkel HG, Winkelmann T (1998) Plant regeneration via somatic embryogenesis from ovules of Cyclamen persicum Mill. Plant Tissue Cult Biotechnol 4:28–34

    Google Scholar 

  • Stasolla C, Bozhkov PV, Chu TM, van Zyl L, Egertsdotter U, Suarez MF, Craig D, Wolfinger RD, von Arnold S, Sederoff RR (2004) Variation in transcript abundance during somatic embryogenesis in gymnosperms. Tree Physiol 24:1073–1085

    CAS  PubMed  Google Scholar 

  • Steward FC, Mapes MO, Smith J (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am J Bot 45:705–708. doi:10.2307/2439728

    Article  Google Scholar 

  • Suprasanna P, Bapat VA (2005) Differential gene expression during somatic embryogenesis. Plant Cell Monographs 2:305–320. doi:10.1007/7089_038

    Article  Google Scholar 

  • Takamura T, Miyajima I (1996) Embryo development in crosses between diploid and tetraploid cyclamen (Cyclamen persicum MILL.). Journal of the Japanese Society of the Horticultural Science 65:113–120

    Article  Google Scholar 

  • Tchorbadjieva MI (2005) Protein markers for somatic embryogenesis. Plant Cell Monographs 11(2):215–234

    Google Scholar 

  • Thibaud-Nissen F, Shealy RT, Khanna A, Vodkin LO (2003) Clustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean. Plant Physiol 132:118–136. doi:10.1104/pp.103.019968

    Article  CAS  PubMed  Google Scholar 

  • Wainwright H, Harwood AC (1985) In vitro organogenesis and plant regeneration of Cyclamen persicum Mill. using seedling tissue. J Hortic Sci 60:397–403

    Google Scholar 

  • Wicart G, Mouras A, Lutz A (1984) Histological study of organogenesis and embryogenesis in Cyclamen persicum Mill. tissue cultures: evidence for a single organogenetic pattern. Protoplasma 119:159–167. doi:10.1007/BF01288870

    Article  Google Scholar 

  • Winkelmann T, Heintz D, Dorsselaer AV, Serek M, Braun HP (2006) Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology. Planta 224:508–519. doi:10.1007/s00425-006-0238-8

    Article  CAS  PubMed  Google Scholar 

  • Ziegleir H, Ziegler I, Schmidt-Clausen HJ (1965) The influence of light intensity and light quality on the increase in activity of the NADP+-dependent glyceraldehyde-3-phosphate-dehydrogenase. Planta (Berl) 67:344–356

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the Key Technologies Program of the office of Science and Technology of Shandong Province (2004GG4202010).

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Authors and Affiliations

  1. College of Chemistry and Biology Technology, Yantai University, Yantai, 264005, People’s Republic of China

    Fuhua Bian, Funing Qu, Xueqin Gong & Cuirong You

  2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, People’s Republic of China

    Caixia Zheng

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  1. Fuhua Bian
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  2. Caixia Zheng
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  3. Funing Qu
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  4. Xueqin Gong
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  5. Cuirong You
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Correspondence to Fuhua Bian.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s11105-010-0188-y

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Bian, F., Zheng, C., Qu, F. et al. Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill. Plant Mol Biol Rep 28, 22–31 (2010). https://doi.org/10.1007/s11105-009-0104-5

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