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Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.

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Abstract

Hippophae rhamnoides L. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and high altitude. Such tolerance invokes much interest in understanding the biology of this plant species and its utilization potential. In this study, analysis of drought stress-responsive proteins in H. rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to drought stress. By using proteomic techniques, proteins, extracted from leaves, were analyzed using two-dimensional electrophoresis and MALDI-TOF MS. Altogether, 55 proteins exhibited changes in abundance under stress. Of these, 13 proteins were identified, including three that disappeared under drought (a putative ABC transporter ATP-binging protein, a heat shock protein HslU, and a hypothetical protein XP-515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein DSM3645–23351, a putative acyl-CoA dehydrogenase, a nesprin-2, and a J-type co-chaperone HSC20), and three that were only detected under drought (a probable nitrogen regulation protein (NtrX), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). These proteins may function in β-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron–sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance biosynthesis. Four proteins (J-type co-chaperone Hsc20, a putative ABC transporter ATP-binging protein, NtrX, and HslU) were deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies to other organisms. These results provide new insights into our understanding of the mechanism of drought tolerance in plants.

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Acknowledgments

The research was supported by the Outstanding Young Scientist Program of the National Natural Science Foundation of China (No. 30525036), the China National Key Program of the International Cooperation for Science and Technology (No. 2005DFA30620), and the Science Foundation for Talents of Guizhou University (No. 701242301).

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Authors and Affiliations

  1. Key Laboratory of Green Pesticide and Agricultural Bioengineering, Chinese Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guiyang, 550025, China

    Gang Xu

  2. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu, 610041, China

    Gang Xu & Chunyang Li

  3. China Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Gang Xu & Chunyang Li

  4. College of Agriculture, Guizhou University, Guiyang, 550025, China

    Yinan Yao

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  1. Gang Xu
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Xu, G., Li, C. & Yao, Y. Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.. Plant Mol Biol Rep 27, 153–161 (2009). https://doi.org/10.1007/s11105-008-0067-y

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