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Adaptation of a Silica Membrane-based Kit for Purification of Sequencing-ready BAC DNA

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Abstract

We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.

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Acknowledgments

This work was supported in part by USDA Grant no. 97121514 and by the Florida Agricultural Experiment Station.

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Authors and Affiliations

  1. Department of Horticultural Sciences and Plant Molecular and Cellular Biology Program, University of Florida, 1143 Fifield Hall, Gainesville, FL, 32611-0690, USA

    C. Eduardo Vallejos & Valerie Jones

  2. Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Ney S. Sakiyama

  3. DNA Sequencing Laboratory, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32611, USA

    Savita Shanker

Authors
  1. C. Eduardo Vallejos
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  2. Ney S. Sakiyama
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  3. Valerie Jones
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  4. Savita Shanker
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Correspondence to C. Eduardo Vallejos.

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Vallejos, C.E., Sakiyama, N.S., Jones, V. et al. Adaptation of a Silica Membrane-based Kit for Purification of Sequencing-ready BAC DNA. Plant Mol Biol Rep 25, 11–16 (2007). https://doi.org/10.1007/s11105-007-0001-8

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  • DOI: https://doi.org/10.1007/s11105-007-0001-8

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