Abstract
Background and aims
Organic fertilizer (OF) is beneficial for crop yield and quality, however, most phosphorus in OF are organic forms, and cannot be utilized until they are hydrolyzed to inorganic phosphate (Pi). Although root-secreted acid phosphatases (APases) play an important role in hydrolyzing organic phosphorus (Po), it is unclear whether they improve the utilization efficiency of OF, and whether purple acid phosphatase (PAP) genes function in plants’ utilization of OF.
Methods
We report a 10-year field study of OF application to rice, and the response of root-secreted APase activity to organic fertilization. Wild type (WT), OsPAP10c overexpressing, and OsPAP10c deficient rice were used to evaluate the role of this gene on the regulation of root-secreted APase activity and its response to OF.
Results
Over 10 years of OF application, the APase activity of rice root secretions increased, over rice that had been fertilized with chemical fertilizer (CF). The APase activity in the rhizosphere soil of OF treated rice increased as well compared to CF treated rice. In PAP10c-OE rice, APase activity in the root secretions was greater than in WT rice, the activity in pap10c rice was less than in WT in OF treatment.
Conclusion
OF application enhances the APase activity of rice root secretions and improves the APase activity in the rhizosphere soil, which facilitates the hydrolysis of Po in OF. This hydrolysis generated sufficient available Pi for rice growth and production. OsPAP10c positively regulates root-secreted APase activity and Po utilization efficiency in rice in OF treated fields.
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Acknowledgements
This work was sponsored by grants from the International (Region) Collaboration and Communication Project between the National Natural Science Foundation of China and National Science Foundation of the United States [41961124005]; the National Natural Science Foundation of China [41875177]; the National Key Research and Development Planning of Ministry and Provincial Linkage Program [2021YFD1700900]; and Australian Research Council (FT210100902).
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All authors contributed to the study. Lixuan Ren and Bingshuang Wang designed the study. Bingshuang Wang, Yan Sun, Ziwei Dai, Yongjian Tang, Yang Wang performed experiments. Bingshuang Wang, Yunsheng Lou, and Lixuan Ren analyzed results and wrote the manuscript. Yunsheng Lou, Guohua Xu, Chuang Wang and Yinglong Chen contributed to revising the manuscript. All authors read and approved the final manuscript.
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Wang, B., Sun, Y., Dai, Z. et al. The role of OsPAP10c on utilization of phosphorus from organic fertilizer in rice. Plant Soil (2024). https://doi.org/10.1007/s11104-024-06702-x
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DOI: https://doi.org/10.1007/s11104-024-06702-x