Abstract
Purpose
Epiphytic mosses play a crucial role in facilitating the decomposition of deadwood and regulating biogeochemical cycling processes in forests. However, the specific impact of epiphytic mosses on the deadwood decomposition process remains unclear.
Methods
We investigate the effect of epiphytic mosses on the changes in microbial community characteristics and physicochemical properties of five decay classes of Pinus massoniana deadwood. To ensure that our findings were not influenced by external environmental factors, we conducted greenhouse cultivation experiments.
Results
The decay class of deadwood and the presence of epiphytic moss had significant effects on total carbon, total nitrogen, carbon-nitrogen ratio, total phosphorus, total potassium, pH, and condensed tannin levels in deadwood. Furthermore, these two factors also significantly influenced the diversity and richness of the microbial community in deadwood. Notably, epiphytic moss exerts a stronger impact on bacterial community composition compared to fungal communities and decreased the complexity of microbial co-occurrence networks in deadwood. Total carbon and condensed tannin content were the most important factors affecting bacterial taxa, and total carbon, pH, total potassium, condensed tannin and cellulose content were the most important factors affecting fungal taxa.
Conclusion
Epiphytic mosses affect the process of deadwood decomposition by altering physicochemical properties and microbial community characteristics within the deadwood. Our study emphasizes the importance of considering the impact of epiphytic mosses in forest management practices aimed at enhancing the degradation of deadwood, with potential implications for promoting ecosystem sustainability.
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References
Ali I, Yuan P, Ullah S, Iqbal A, Zhao Q, Liang H (2022) Biochar amendment and nitrogen fertilizer contribute to the changes in soil properties and microbial communities in a paddy field. Front Microbiol 13:834751. https://doi.org/10.3389/fmicb.2022.834751
Araujo ASF, Oliveira LMDS, Melo VMM, Antunes JEL, Araujo FF, Mendes LW (2021) Distinct taxonomic composition of soil bacterial community across a native gradient of Cerrado-ecotone-Caatinga. Appl Soil Ecol 161:103874. https://doi.org/10.1016/j.apsoil.2020.103874
Baldrian P (2017) Microbial activity and the dynamics of ecosystem processes in forest soils. Curr Opin Microbiol 37:128–134. https://doi.org/10.1016/j.mib.2017.06.008
Baldrian P, Zrůstová P, Tláskal V, Davidová A, Merhautová V, Vrška T (2016) Fungi associated with decomposing deadwood in a natural beech-dominated forest. Fungal Ecol 23:109–122. https://doi.org/10.1016/j.funeco.2016.07.001
Bargagli R (2016) Moss and lichen biomonitoring of atmospheric mercury: a review. Sci Total Environ 572:216–231. https://doi.org/10.1016/j.scitotenv.2016.07.202
Bello A, Han Y, Zhu HF, Deng LT, Yang W, Meng QX (2020) Microbial community composition, co-occurrence network pattern and nitrogen transformation genera response to biochar addition in cattle manure maize straw composting. Sci Total Environ 721:137759–137759. https://doi.org/10.1016/j.scitotenv.2020.137759
Cao W, Xiong YX, Zhao DG, Tan HY, Qu JJ (2020) Bryophytes and the symbiotic microorganisms, the pioneers of vegetation restoration in karst rocky desertification areas in southwestern China. Appl Microbiol Biotechnol 104:873–891. https://doi.org/10.1007/s00253-019-10235-0
Čapek P, Choma M, Tahovská K, Kaňa J, Kopáček J, Šantrůčková H (2021) Coupling the resource stoichiometry and microbial biomass turnover to predict nutrient mineralization and immobilization in soil. Geoderma 385:0016–7061. https://doi.org/10.1016/j.geoderma.2020.114884
Chaer G, Fernandes M, Myrold D, Bottomley P (2009) Comparative resistance and resilience of soil microbial communities and enzyme activities in adjacent native forest and agricultural soils. Microb Ecol 58:414–424. https://doi.org/10.1007/s00248-009-9508-x
Chang CH, Wu FZ, Yang WQ, Tan B, Xiao S, Li J, Gou XL (2014) The dynamics of microbial community structure at different stages of log decay in an alpine forest of western Sichuan. Chinese Journal of Applied & Environmental Biology 20(6):978–985. https://doi.org/10.17521/cjpe.2015.0002
Chang CH, Wu FZ, Wang Z, Tan B, Cao R, Yang WQ, Cornelissen JHC (2019) Effects of Epixylic vegetation removal on the dynamics of the microbial community composition in decaying logs in an Alpine Forest. Ecosystems 22:1478–1496. https://doi.org/10.1007/s10021-019-00351-3
Chen S, Zhou Y, Chen Y, Gu JJB (2018) fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34(17):i884–i890. https://doi.org/10.1093/bioinformatics/bty560
Coyte KZ, Schluter J, Foster KR (2015) The ecology of the microbiome: networks, competition, and stability. Science 350(6261):663–666. https://doi.org/10.1126/science.aad2602
Cui FX, Yang LB, Sui X, Huang QY, Zhu DG (2021) Analysis on fungal composition and diversity difference of Larix gmelinii deadwood during the decomposition process in cold temperate zone. Journal of Central South University of Forestry & Technology 41(08):36–44. https://doi.org/10.14067/j.cnki.1673-923x.2021.08.005
Du M, Zhang J, Wang G, Liu C, Wang Z (2022) Response of bacterial community composition and co-occurrence network to straw and straw biochar incorporation. Front Microbiol 13:999399. https://doi.org/10.3389/fmicb.2022.999399
Edgar RC (2013) UPARSE: highly accurate OTU sequences from microbial amplicon reads. 10(10), 996-998. Nat Methods 10(10):996–998. https://doi.org/10.1038/nmeth.2604
Fan H, Sun LW, Yang LG, Zhou JC, Yin PP, Li K, Xue Q, Li X, Liu YJ (2018) Assessment of the bioactivephenolic composition of Acer truncatum seed coat as a byproductof seed oil. Ind Crop Prod 118:11–19. https://doi.org/10.1016/j.indcrop.2018.03.030
Fukasawa Y, Takahashi K, Arikawa T, Hattori T, Maekawa N (2015) Fungal wood decomposer activities influence community structures of myxomycetes and bryophytes on coarse woody debris. Fungal Ecol 14:44–52. https://doi.org/10.1016/j.funeco.2014.11.003
Groß C, Hossen S, Dittrich S, Knorr K-H, Borken W, Noll M (2023) Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests. Environ Microbiol 1–17. Available from: https://doi.org/10.1111/1462-2920.16555
Gu Y, Wang J, Cai W, Li G, Mei Y, Yang S (2021) Different amounts of nitrogen fertilizer applications Alter the bacterial diversity and community structure in the rhizosphere soil of sugarcane. Front Microbiol 12:721441. https://doi.org/10.3389/fmicb.2021.721441
Harmon ME, Franklin JF, Swanson FJ, Sollins P, Gregory SV, Lattin JD, Anderson NH, Cline SP, Aumen NG, Sedell JR, Lienkaempeer GW, Cromack K, Cummins KW (1986) Ecology of coarse woody debris in temperate. Ecosystems 15:133–302
Haughian SR, Frego KA (2017) Log moisture capacity does not predict epixylic bryophyte growth under thinned and unthinned forest canopies. Funct Ecol 31(8):1540–1549. https://doi.org/10.1111/1365-2435.12864
Jiao S, Peng Z, Qi J, Gao J, Wei G (2021) Linking bacterial-fungal relationships to microbial diversity and soil nutrient cycling. mSystems 6(2):e01052–e01020. https://doi.org/10.1128/mSystems.01052-20
Johnston SR, Boddy L, Weightman AJ (2016) Bacteria in decomposing wood and their interactions with wood-decay fungi. FEMS Microbiol Ecol 92(11):fiw179. https://doi.org/10.1093/femsec/fiw179
Klavina D, Pennanen T, Gaitnieks T, Velmala S, Lazdins A, Lazdina D, Menkis A (2016) The ectomycorrhizal community of conifer stands on peat soils 12 years after fertilization with wood ash. Mycorrhiza 26:153–160. https://doi.org/10.1007/s00572-015-0655-2
Kovářová M, Pyszko P, Plášek V (2022) How does the pH of tree bark change with the presence of the epiphytic bryophytes from the family Orthotrichaceae in the interaction with trunk inclination? Plants 11:63. https://doi.org/10.3390/plants11010063
Li M, Ding GJ, Sun XG, Luo XM, Zhang RB (2016) Plant diversity and soil enzyme activity in 4 typical communities of Pinus massoniana in Guizhou. Journal of Forest and Environment 36(04):434–441. https://doi.org/10.13324/j.cnki.jfcf.2016.04.009
Li C, Li X, Shi Y, Yang Y, Li H (2022) Effects of nitrogen addition on soil carbon-fixing microbial diversity on different slopes in a degraded Alpine meadow. Front Plant Sci 13:921278. https://doi.org/10.3389/fpls.2022.921278
Liu C, Zhao D, Ma W, Guo Y, Wang A, Wang Q, Lee DJ (2016) Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp. Appl Microbiol Biotechnol 100(3):1421–1426. https://doi.org/10.1007/s00253-015-7039-6
Liu L, Fan YJ, Song XT, Li M, Sho XM, Wang XR (2020) Bryophyte societies on the fallen logs of Pinus armandii with different decay classes in Sygera Mountains. Chinese Journal of Plant Ecology 44(8):1–12 https://kns.cnki.net/kcms/detail/11.3397.Q.20201112.1525.018.html
Liu W, Liu L, Yang X, Deng M, Wang Z, Wang P (2021) Long-term nitrogen input alters plant and soil bacterial, but not fungal beta diversity in a semiarid grassland. Glob Chang Biol 27(16):3939–3950. https://doi.org/10.1111/gcb.15681
Liu SQ, Yang R, Peng XD, Hou CL, Ma JB, Guo JR (2022) Contributions of plant litter decomposition to soil nutrients in ecological tea gardens. Agriculture 12:957. https://doi.org/10.3390/agriculture12070957
Magnússon RÍ, Tietema A, Cornelissen JHC, Kalbitz K (2016) Tamm review: sequestration of carbon from coarse woody debris in forest soils. For Ecol Manag 377:1–15. https://doi.org/10.1016/j.foreco.2016.06.033
Magoč T, Salzberg SL (2011) FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27:2957–2963. https://doi.org/10.1093/bioinformatics/btr507
Mežaka A, Brūmelis G, Piterāns A (2012) Tree and stand-scale factors affecting richness and composition of epiphytic bryophytes and lichens in deciduous woodland key habitats. Biodivers Conserv 21:3221–3241. https://doi.org/10.1007/s10531-012-0361-8
Mieszkin S, Richet P, Bach C, Lambrot C, Augusto L, Buée M, Uroz S (2021) Oak decaying wood harbors taxonomically and functionally different bacterial communities in sapwood and heartwood. Soil Biol Biochem 155:108160. https://doi.org/10.1016/j.soilbio.2021.108160
Moroni MT, Morris DM, Shaw C, Stokland JN, Harmon ME, Fenton NJ, Merganicová K, Mergani J, Okabe K, Hagemann U (2015) Buried wood: a common yet poorly documented form of deadwood. Ecosystems 18:605–628. https://doi.org/10.1007/s10021-015-9850-4
Přívětivý T, Janík D, Unar P, Adam D, Král K, Vrška T (2016) How do environmental conditions affect the deadwood decomposition of european beech (fagus sylvatica l.)? For Ecol Manag 381:177–187. https://doi.org/10.1016/j.foreco.2016.09.033
Putna S, Mežaka A (2014) Preferences of epiphytic bryophytes for forest stand and substrate in north-East Latvia. Folia Cryptogamica Estonica 51:75–83. https://doi.org/10.12697/fce.2014.51.08
Ricker MC, Lockaby BG, Blosser GD, Conner WH (2016) Rapid wood decay and nutrient mineralization in an old-growth bottomland hardwood forest. Biogeochemistry 127:323–338. https://doi.org/10.1007/s10533-016-0183-y
Romashkin I, Shorohova E, Kapitsa E, Galibina N, Nikerova K (2018) Carbon and nitrogen dynamics along the log bark decomposition continuum in a Mesic old-growth boreal forest. Eur J For Res 137:643–657. https://doi.org/10.1007/s10342-018-1131-2
Shi BY, Wang XR, Chen HM, Yang HY (2022a) Growth and physiological responses of Acanthopanax runner moss to different cultivation substrates. Northwest J Bot (07):1208–1218. https://doi.org/10.13605/j.cnki.52-1065/s.2022.04.027
Shi BY, Wang XR, Yang SY, Chen HM, Zhao Y (2022b) Addition Pinus massoniana deadwood improved the growth of Plagiomnium acutum in a substrate cultivation. Sci Rep 12:17755. https://doi.org/10.1038/s41598-022-21901-1
Shorohova E, Kapitsa E, Kazartsev I, Romashkin I, Polevoi A, Kushnevskaya H (2016) Tree species traits are the predominant control on the decomposition rate of tree log bark in amesic old-growth boreal forest. For Ecol Manag 377:36–45. https://doi.org/10.1016/j.foreco.2016.06.036
Song L, Lu HZ, Xu XL, Li S, Shi XM, Chen X, Wu Y, Huang JB, Chen Q, Liu S, Wu CS, Liu WY (2016) Organic nitrogen uptake is a significant contributor to nitrogen economy of subtropical epiphytic bryophytes. Sci Rep 6:30408. https://doi.org/10.1038/srep30408
Song S, Xiong K, Chi Y, He C, Fang J, He S (2022) Effect of cultivated pastures on soil bacterial communities in the karst rocky desertification area. Front Microbiol 13:922989. https://doi.org/10.3389/fmicb.2022.922989
Souza E, Silva JB, Pinto AS, Lopes S (2021) Soil texture and functional traits of trees structure communities of epiphytic mosses in a tropical dry forest. Flora 283:151924. https://doi.org/10.1016/j.flora.2021.151924
Ulyshen MD (2016) Wood decomposition as influenced by invertebrates. Biol Rev 91:70–85. https://doi.org/10.1111/brv.12158
Vitt DH, Finnegan L, House M (2019) Terrestrial bryophyte and lichen responses to canopy opening in pine-Moss-lichen forests. Forests 10:233. https://doi.org/10.3390/f10030233
Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73(16):5261–5267. https://doi.org/10.1128/AEM.00062-07
Wang B, Wu F, Xiao S, Yang W, Justine MF, He J, Tan B (2016) Effect of succession gaps on the understory water-holding capacity in an over-mature alpine forest at the upper reaches of the Yangtze River. Hydrol Process 30(5):692–703. https://doi.org/10.1002/hyp.10613
Wang Z, Wu FZ, Yang WQ, Tan B, Chang CH, Wang Q, Cao R, Tang GQ (2018) Effect of gap position on the heavy metal contents of epiphytic mosses and lichens on the fallen logs and standing trees in an Alpine Forest. Forests 9(7):383. https://doi.org/10.3390/f9070383
Wicaksono WA, Cernava T, Berg C, Berg G (2021) Bog ecosystems as a playground for plant–microbe coevolution: bryophytes and vascular plants harbour functionally adapted bacteria. Microbiome 9:170. https://doi.org/10.1186/s40168-021-01117-7
Yan ER, Wang XH, Huang JJ (2005) Concept and classification of coarse woody debris in forest ecosystems. Acta Ecol Sin 25(1):158–167
Yang LB, Cui FX, Huang QY, Zhu DG, Xu F (2021) Analysis on bacterial diversity and composition of Larixg melinii deadwood with different decay classes. J Cent South Univ For Technol 93–100, 182. https://doi.org/10.14067/j.cnki.1673-923x.2021.04.011
Yang Y, Bao X, Xie H, He H, Zhang X, Shao P et al (2022) Frequent Stover mulching builds healthy soil and sustainable agriculture in Mollisols. Agric Ecosyst Environ 326:107815. https://doi.org/10.1016/j.agee.2021.107815
Żarnowiec J, Staniaszek-Kik M, Chmura D (2021) Trait-based responses of bryophytes to the decaying logs in central European mountain forests. Ecol Indic 126:1470–160X. https://doi.org/10.1016/j.ecolind.2021.107671
Zhang ML, Zhang X, Zhang LY, Zeng L, Liu Y, Wang XB, He P, Li ST, Liang JQ, Zhou W, Ai C (2021) The stronger impact of inorganic nitrogen fertilization on soil bacterial community than organic fertilization in short-term condition. Geoderma 382:114752. https://doi.org/10.1016/j.geoderma.2020.114752
Zhou JZ, Deng Y, Luo F, He ZL, Tu QC, Zhi XY (2010) Functional molecular ecological networks. MBio 1(4):10–1128. https://doi.org/10.1128/mBio.00169-10
Zuo J, Berg MP, Klein R, Nusselder J, Neurink G, Decker O, Hefting MM, Sass-Klaassen U, van Logtestijn RSP, Goudzwaard L, van Hal J, Sterck FJ, Poorter L, Cornelissen JHC (2016) Faunal community consequence of interspecific bark trait dissimilarity in early-stage decomposing logs. Funct Ecol 30(12):1957–1966. https://doi.org/10.1111/1365-2435.12676
Acknowledgements
This research was funded by the National Natural Science Foundation of China, “Landscape Adaptation Evaluation of Bryophytes in Karst Areas and its Landscape Theory” (31960328), National Natural Science Foundation of China (32060353) and the Science and Technology Talent Platform Project of Guizhou Province ([2018] 5261).
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Bingyang Shi: Conceptualization, Data curation, Methodology, Resources and Writing—original draft. Xiurong Wang: Conceptualization. Xiurong Wang and Yang Zhao: Funding acquisition, Writing—review & editing. Shuoyuan Yang, Hongmei Chen, Qiao Liu, Rong Zou, Muyan Xie, Fang Liao, Lixin Duan: Investigation, Resources.
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Shi, B., Wang, X., Yang, S. et al. Epiphytic mosses alter Pinus massoniana deadwood microbial and physicochemical properties thereby influencing the decomposition process. Plant Soil (2024). https://doi.org/10.1007/s11104-024-06652-4
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DOI: https://doi.org/10.1007/s11104-024-06652-4