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Jasmonic acid participating in the systemic regulation of phosphate starvation response in Brassica napus

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Abstract

Aims

The aims of this work were to investigate phosphate starvation responses of Brassica napus (B. napus) under heterogeneous phosphate (Pi) supply and the regulatory role of jasmonic acid (JA) in the systemic response to Pi starvation.

Methods

A split-root system with two separated compartments was employed to mimic heterogeneous Pi distribution in the soil and to examine the effect of heterogeneous Pi supply, and JA or DIECA (JA biosynthesis inhibitor) on growth, root morphology, Pi concentration, Acid phosphatase (APase) activity, nutrition uptake, JA concentration and expression of Pi starvation systemically-induced (PSSI) genes of B. napus.

Results

Heterogeneous Pi supply systemically modified root morphology that increased the total root surface area (TRSA), total root volume (TRV), total root length (TRL) and total lateral root number (TLRN) of root with local Pi supply (R +) and decreased them of root with local no Pi supply (R-) when compared to root with homogeneous Pi supply (R + +) and root devoid of Pi (R–), respectively. Anthocyanin, APase activity and JA concentration in shoot and root of B. napus were systemically regulated by heterogeneous Pi supply. In addition, heterogeneous Pi supply significantly promoted nutrient uptake when compared with homogeneous no Pi supply. Root morphology of B. napus was significantly changed by exogenous addition of JA or DIECA in a split-root system. JA enhanced Pi starvation response by inducing expression of PSSI genes in shoots and roots.

Conclusions

Our results suggest that JA enhances systemic Pi starvation response of B. napus by regulating root morphology, Pi homeostasis and inducing expression of PSSI genes under heterogeneous Pi supply.

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Data Availability

All data supporting the findings of this study are available within the paper and within its supplementary data published online.

Abbreviations

Pi:

Phosphate

JA:

Jasmonic acid

DIECA:

Diethyldithiocarbamic acid

APase:

Acid phosphatase

PSSI:

Pi starvation systemically-induced

TRSA:

Total root surface area

TRV:

Total root volume

TRL:

Total root length

TLRN:

Total lateral root number

P:

Phosphorus

JA-Ile:

Jasmonoyl-L-isoleucine

PR:

Primary root

LR:

Lateral root

1°LR:

First-order lateral root

2°LR:

Second-order lateral root

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Acknowledgements

We thank Dr. Feng Ren (Central China Normal University, Wuhan, China) for kindly providing us with seeds of transgenic A. thaliana pBnPht1;4-GUS. This work was supported by the National Nature Science Foundation of China (Grant No. 31972498 and 32172662). Sergey Shabala acknowledges a support from the Australian Research Council (project LP200200132).

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Author notes

  1. Yalin Li and Xinyu Yang contributed equally to this work.

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Yalin Li, Xinyu Yang, Chuang Wang, Guangda Ding, Fangsen Xu, Sheliang Wang, Hongmei Cai & Lei Shi

  2. International Research Center for Environmental Membrane Biology & Department of Horticulture, Foshan University, Foshan, 528000, China

    Yalin Li, Xuewen Li, Sergey Shabala & Min Yu

  3. Microelement Research Center/Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China

    Yalin Li, Xinyu Yang, Chuang Wang, Guangda Ding, Fangsen Xu, Sheliang Wang, Hongmei Cai & Lei Shi

  4. School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK

    John P. Hammond

  5. School of Biological Sciences, University of Western Australia, Crawley, WA6009, Australia

    Sergey Shabala

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  1. Yalin Li
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Correspondence to Lei Shi.

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Li, Y., Yang, X., Li, X. et al. Jasmonic acid participating in the systemic regulation of phosphate starvation response in Brassica napus. Plant Soil (2023). https://doi.org/10.1007/s11104-023-06355-2

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