Abstract
Background and aims
In Morocco's semi-arid and sub-humid climates, the fodder legume Lupinus luteus is cultivated for its high economic and ecological value. In this work, we characterized some microsymbionts of L. luteus isolated by trapping from plants grown in soils of the agricultural area of Zaer, Morocco.
Methods
The phenotypic and genotypic diversity, the plant growth-promoting abilities, and the symbiotic efficiency of rhizobia isolated from root nodules of L. luteus were analyzed.
Results
Based on their REP-PCR fingerprinting results, eighteen strains were selected for Multilocus sequence analysis (MLSA) using rrs, glnII, gyrB, recA, and rpoB housekeeping genes, which revealed that all the strains belong to the genus Bradyrhizobium. Some strains were close to B. lupini and B. canariense. However, the remaining strains grouped apart from all described Bradyrhizobium species. Phylogenetic analysis of the nodA and nodC symbiotic genes showed that all the strains are members of the symbiovar genistearum. Quantitative evaluation of selected plant growth-promoting activities showed that the strains solubilize phosphate, and produce auxins and siderophores. All the strains used as inoculum in greenhouse experiments significantly improved the growth of L. luteus under nitrogen-free conditions.
Conclusions
Bradyrhizobium lupini and B. canariense are the main rhizobia nodulating L. luteus in the Zaer region. In addition to their high nitrogen fixation efficiency, these isolates also exhibit plant growth-promoting activities. These results highlighted one of the major reasons for the success of yellow lupine in this area without nitrogen fertilizers and pointed to the possibility of formulating these rhizobia into an effective inoculum for L. luteus.
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Acknowledgements
The authors would like to thank all those who contributed to the realization of this work. This work was carried out in the framework of the multilateral project between Morocco, Algeria, Tunisia and France, entitled PHC-Maghreb 34MAG21—45983TE. Miss Zohra Chaddad also received an Excellence Grant for her doctoral thesis from the Moroccan Ministry of Higher Education.
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Z. Chaddad carried out all the experiments and participated in the analysis, interpretation of the data, and writing the manuscript. M. Lamrabet, O. Bouhnik, A. Sportes, S. Alami, H. Lamin, K. Kadouri, and M. Bennis participated in the sampling, acquisition and interpretation of the results. B. Mnasri, D. Wipf, and H. Abdelmoumen contributed to the conception of the work, and revision of the manuscript. P. E. Courty participated in the conception of the work, the drafting and the revision of the manuscript. M. Missbah El Idrissi conceived and designed the work, participated in the interpretation of the data, drafting of the manuscript, and its revision. All authors contributed to the final version of the article.
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Chaddad, Z., Lamrabet, M., Bouhnik, O. et al. Genetic diversity, phenotypic traits, and symbiotic efficiency of native Bradyrhizobium strains of Lupinus luteus in Morocco. Plant Soil 493, 407–426 (2023). https://doi.org/10.1007/s11104-023-06236-8
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DOI: https://doi.org/10.1007/s11104-023-06236-8