Abstract
Background and aims
Low nitrogen (N) soil environment is one of the main factors limiting crop yield and horticultural plant growth. Dark septate endophytes (DSEs) are co-occurring in 80% of terrestrial plants and they can promote the absorption of N. However, few data on the contribution of DSEs symbiosis to plant N acquisition and their molecular mechanisms are available.
Methods
The growth, N contents and expression of N related genes of Gisela 5, a sweet cherry rootstock colonized with and without DSE fungus S16, were measured under low and normal nitrate conditions. Subsequently, molecular identification and functional study of PaNRT2.1, a nitrate transporter highly responsive to S16, were conducted.
Results
In this study, we found that S16 promoted the growth and N uptake of Gisela 5 seedlings under low and normal nitrate conditions, and induced the expression of the nitrate transporter gene PaNRT2.1 in roots. The expression of PaNRT2.1 significantly affected the nitrate uptake of Gisela 5 roots and the symbiosis with S16 under low nitrate conditions. Transcriptome analysis showed that a receptor-like kinase gene PaCRK10 was significantly induced during S16 symbiosis and PaNRT2.1 overexpression, indicating a signal transduction between DSE colonization with nitrate uptake. Further studies showed that there was a strong interaction between PaNRT2.1 and PaCRK10.
Conclusions
Taken together, our results provide a deeper understanding of how DSE promoted N uptake by sweet cherry rootstock Gisela 5 and a more promising approach for improving N uptake efficiency through plant–microbe interactions.
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Data availability
The RNA-Seq data has been deposited to the SRA of NCBI (https://www.ncbi.nlm.nih.gov/biosample) with the data set BioProject ID PRJNA896536. All data supporting the findings of this study are available within the paper and within its supplementary materials published online.
Change history
16 June 2023
A Correction to this paper has been published: https://doi.org/10.1007/s11104-023-06117-0
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (32171810; 32071788; 32000090), the Youth Innovation Technology Support Planning Project for Institution of Higher Education of Shandong Province (2022KJ121), the Improved Seed Project of Shandong Province (2020LZGC008), the Natural Science Foundation of Shandong Province (ZR2022QD136; ZR2021MC102; ZR2020MC153) and the Key Research and Development Program of Yantai City, Shandong Province, China (2022XDRH010).
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LW and HYS conceived and designed the project. FLW and DHQ performed most of the experiments. XHZ, DZZ and XZ contributed reagents or provided laboratory assistance. FLW interpreted results and wrote the manuscript.
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Fanlin Wu, Dehui Qu Co-first Author and The two authors contributed equally to this work.
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Wu, F., Qu, D., Zhao, X. et al. A high-affinity nitrate transporter PaNRT2.1 mediates dark septate endophyte (DSE) dependent nitrogen assimilation in sweet cherry roots. Plant Soil 489, 539–556 (2023). https://doi.org/10.1007/s11104-023-06039-x
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DOI: https://doi.org/10.1007/s11104-023-06039-x