Abstract
Purpose
This work integrated field investigation results with laboratory experiments to investigate the interaction of rhizosphere soil microorganisms in the continuous cropping of Panax notoginseng and saponins, providing a scientific foundation for overcoming P. notoginseng continuous cropping obstacles.
Methods
The concentrations of saponins were measured using UPLC–MS/MS, and the microbes in the P. notoginseng rhizosphere soil were sequenced using the Illumina MiSeq high-throughput platform.
Results
The concentrations of ginsenoside Rd, ginsenoside Rg2, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and notoginsenoside R1 in the rhizospheric soil of infected P. notoginseng increased considerably. The P. notoginseng rhizospheric soil rapidly degraded saponins in the following order: ginsenoside Rd > ginsenoside Rg1 > notoginsenoside R1 > ginsenoside Re > ginsenoside Rg2 > ginsenoside Rb1. Saponins substantially enhanced the fungal Chao1 index, and Rd had the greatest effect. Saponins shifted the microbial community architecture and abundance in the rhizosphere soil. Ginsenoside Re stimulated the growth of Rhodotorula and Alternaria, ginsenoside Rg1 significantly increased the production of Spizellomyces, ginsenoside Rd promoted the proliferation of Aureobasidium, and notoginsenoside R1 fostered the growth of Alternaria. Further investigations showed that increases in Plectosphaerella and Clonostachys were directly and positively related to notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1, while an increase in Ilyonectria was strongly and positively related to ginsenoside Rb1 and ginsenoside Rg2.
Conclusions
The six saponins have the potential to shape the structure of the microbial community of the P. notoginseng rhizospheric soil, and enrichment in particular saponins lays the foundation for the emergence and proliferation of pathogenic fungi.
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Data availability
The datasets generated during the current study are available in the NCBI repository: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA808393.
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Acknowledgements
We are grateful to Guangzhou Gene Denovo Biotechnology Co., Ltd., for assisting with sequencing and bioinformatics analysis.
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This work was financially supported by the National Natural Science Foundation of China (Grant nos. 31960630 and 31960232).
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All authors contributed to the study conception and design. Materials preparation, data collection and analysis were performed by Limei Bao, Yuyan Liu, Jinmiao Chen, Yafang Ding, Junjie Shang, Jinhua Li, Yunlin Wei, Yong Tan and Futing Zi. The first draft of the manuscript was written by Limei Bao, and all the authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.
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Bao, L., Liu, Y., Chen, J. et al. Different effects of six saponins on the rhizosphere soil microorganisms of Panax notoginseng. Plant Soil 487, 389–406 (2023). https://doi.org/10.1007/s11104-023-05934-7
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DOI: https://doi.org/10.1007/s11104-023-05934-7