Abstract
Purpose
The root microbiome accompanies plants throughout their growth cycle, helping plants absorb nutrients, resist disease and adapt to stressful environments. It is important to reveal the interaction and assembly of the forest root microbiome and the mechanism of its response to spatial variation.
Methods
We investigated the diversity, community assembly, and network associations of microbial communities in the endosphere and rhizosphere of Robinia pseudoacacia in three counties in the forest distribution area of the Loess Plateau using 16S rRNA gene high-throughput sequencing technology.
Results
Environmental factors affected the recruitment of root-associated microorganisms, and less mean annual precipitation (MAP) increased the difference between the rhizosphere and endosphere microorganisms. As MAP decreased, the co-occurrence network stability of the root fungal community was disrupted, while network instability of bacteria was not manifested. At the same time, MAP reduction reduced the external connectivity of different compartments of root-associated bacteria and fungi. Moreover, the assembly of the rhizosphere soil bacterial and endosphere fungal communities was dominated by stochastic processes, while the assembly of the endosphere bacterial and rhizosphere fungal communities was dominated by deterministic processes. As MAP decreases, the randomness of the assembly process of rhizosphere soil bacteria was significantly reduced.
Conclusions
The root -associated microbial communities of Robinia pseudoacacia have different sensitivities and assembly mechanisms to spatial variation. MAP is the main factor driving the stability, connectivity and assembly of root microbial communities of Robinia pseudoacacia in the Loess Plateau.
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Data availability
The datasets analysed during the current study are available in the NCBI SRA database, [PRJNA838064].
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Acknowledgements
We would like to express our sincere gratitude for the help from the staff of Shaanxi Huanglong Mountain Forest Ecosystem National Positioning Observation and Research Statio during the field survey and sampling.
Funding
This work was financially supported by the National Key Research and Development Program of China named “Quality improvement technology of low-efficiency plantation forest ecosystem in the Loess Plateau” [grant number 2022YFF1300400]; Subject: Multifunctional enhancement of Robinia pseudoacacia forests in hilly and gully areas and techniques for maintaining vegetation stability on the Loess Plateau [grant number 2022YFF1300405]; Special Topic: Distribution Pattern, Causes and Change Trends of Low-Efficiency Robinia pseudoacacia plantations.
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Zhao Zhong contributed to the study conception and design. Material preparation, data collection and analysis were performed by Ku Yongli, Han Xiaoting, Lei Yuting and Zhang Mei. The first draft of the manuscript was written by Ku Yongli. All authors read and approved the final manuscript.
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Ku, Y., Han, X., Lei, Y. et al. Different sensitivities and assembly mechanisms of the root-associated microbial communities of Robinia pseudoacacia to spatial variation at the regional scale. Plant Soil 486, 621–637 (2023). https://doi.org/10.1007/s11104-023-05897-9
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DOI: https://doi.org/10.1007/s11104-023-05897-9