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Quantitative reduction of soil bacteria and qualitative microbial changes: biotic components associated to kiwifruit decline

Abstract

Purpose

This study aimed at elucidating the biotic components of crop decline affecting kiwifruit (Actinidia deliciosa) orchards.

Methods

The study was carried out on soil samples originating from an over twenty-year-old orchard showing typical yield decline (Old), one in full production phase (Adult), one fallow after a kiwifruit cultivation (Fallow), an abandoned one (Virgin). Soil health of those soil samples was assessed with an in-pot growth assay using kiwifruit plantlets in which root endophytic fungi and rhizosphere bacteria communities were assessed using qPCR and NGS analysis.

Results

Plant growth in the Old field was significantly lower than the others, in line with the crop decline of that field. The Old treatment differed from the others in the following soil features: i. a great reduction of total bacteria, Pseudomonas, actinomycetes and Bacillus compared to the Adult orchard; ii. a significant increase of Nitrosospira and other nitrifying bacteria which persisted in kiwifruit rhizosphere even under the optimal conditions; iii. a reduction of potentially beneficial genera among which Massilia, Rubrobacter and Kaistobacter. Old, Adult and Fallow were similar in root fungal community composition, with Dactylonectria as dominant genus (about 50%); whilst in the Virgin prevailed saprophytic non-pathogenic fungi.

Conclusions

Bacterial communities in over-30-year-old kiwifruit orchards were greatly reduced and modified, thus suggesting being a cause of the reduced ability of soil to support plant growth. In addition, kiwifruit manifested a legacy effect on soil-borne fungal communities, including root endophytes.

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Acknowledgements

The authors wish to thank the technicians Gialunca Cembali e Gianluca Valenti of the Agrointesa Soc. Coop for they support in this study.

This research was funded by Emilia Romagna region within the Rural Development Plan 2014-2020, with the SOSFERA Project https://progetti.crpv.it/Home/ProjectDetail/65

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Authors and Affiliations

Authors

Contributions

Manici L.M.: Conceptualization; methodology, formal analysis and writing -original draft. Saccà M.L.: methodology, formal analysis, writing- review & editing. Scotti C.: Investigation in pedological aspects. Caputo F.: Investigation and methodology.

Corresponding author

Correspondence to Luisa Maria Manici.

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Conflicts of Interest

The authors declare no conflict of interest.

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Manici, L.M., Saccà, M.L., Scotti, C. et al. Quantitative reduction of soil bacteria and qualitative microbial changes: biotic components associated to kiwifruit decline. Plant Soil (2022). https://doi.org/10.1007/s11104-022-05470-w

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  • DOI: https://doi.org/10.1007/s11104-022-05470-w

Keywords

  • Actinidia deliciosa
  • Dactylonectria torresensis
  • Nitrifying bacteria, Nitrosospira
  • Growth reduction