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How encroaching shrubs and nutrients affect N2-fixation in the Chihuahuan desert

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Abstract

Background and aims

Organisms that are capable of nitrogen (N) fixation, such as some species of cyanobacteria in biological soil crusts (biocrusts), can be ecologically important especially in low-nutrient dryland ecosystems. In addition, woody plant encroachment is occurring in drylands globally, including many species of N2-fixing shrubs and trees. We determined the effects of nutrient deposition and shrub encroachment by Larrea tridentata on N2-fixation rates of biocrusts and compared these rates to another encroaching shrub, the N2-fixer Prosopis glandulosa in the northern Chihuahuan Desert.

Methods

We used both the acetylene reduction assay (ARA) and the 15N natural abundance techniques to determine N2-fixation rates of biocrusts. We also assessed the effects of nutrient additions on biocrust N2-fixation rates using a long-term N addition experiment started in 2008 that is part of the Nutrient Network (NutNet).

Results

There was no correlation between the ARA and δ15N methods. We found that P. glandulosa likely fixes more N (at least during the spring) than biocrusts based on δ15N content in roots. There was no significant difference in δ15N between P. glandulosa and our reference plant, L. tridentata. δ15N declined in N-fertilized plots presumably because biological nitrogen fixation (BNF) is costly or because added N has negative effects on microbial communities.

Conclusions

Biocrusts fixed less nitrogen than N2-fixing legume shrubs during this sampling period. Nonetheless, biocrusts are still a significant contributor to N2-fixation in these nutrient-poor drylands based on the large proportion of soil surface area occupied by these organisms.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

The code generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Notes

  1. janderso@jornada-vmail.nmsu.edu.

  2. Vince.gutschick@gmail.com.

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Acknowledgements

Many thanks to Christian Combs for assistance in both the field and the laboratory. We thank John Anderson of the Jornada LTER for his landscape insight and help coordinating the study. We thank Stephanie Baker of the Sevilleta LTER for assistance with plant identifications. We also thank Ben Spector for his technical assistance and Erika Bolin for running the gas chromatograph samples. We thank Drs. Bade, Blackwood, Case and Mou for the use of laboratory equipment at Kent State University. We also thank Dr. Chris Blackwood of reviewing this manuscript. This project was funded by the Herrick Trust, Kent State University. Partial support provided by DEB-1655499 for Long-term Ecological Research.

Funding

This study was funded by the Herrick Trust, Kent State University.

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Authors and Affiliations

  1. Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA

    Lauren M. Baldarelli & David Ward

  2. Department of Biology, University of New Mexico, Albuquerque, NM, 87131, USA

    Scott L. Collins

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  1. Lauren M. Baldarelli
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Correspondence to Lauren M. Baldarelli.

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Baldarelli, L.M., Collins, S.L. & Ward, D. How encroaching shrubs and nutrients affect N2-fixation in the Chihuahuan desert. Plant Soil 466, 545–556 (2021). https://doi.org/10.1007/s11104-021-05072-y

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