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Evidence for nickel mobilisation in rhizosphere soils of Ni hyperaccumulator Odontarrhena serpyllifolia

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Abstract

Purpose

Rhizosphere processes are known to modify uptake of elements from soil, but limited information is available for hyperaccumulators. We investigated labile Ni fractions and their kinetics of replenishment in the rhizospheres of the Ni-hyperaccumulator Odontarrhena serpyliffolia, the Ni-excluder Holcus lanatus and in bulk soils collected at the same serpentine outcrop.

Methods

Labile Ni fractions in rhizosphere and bulk soil were evaluated using conventional extractions and also by the Diffusive Gradients in Thin Films (DGT) technique. DGT data were used to predict the kinetics of Ni resupply using DIFS modelling. Chemical imaging of Ni distribution along roots using DGT coupled with laser-ablation was conducted.

Results

Labile Ni forms were higher in both rhizosphere than in bulk soils, together with an increase in dissolved organic C, cation exchange capacity and the Ca/Mg ratio. Ni fractionation indicated a shift towards less stable Ni fractions in the rhizosphere, particularly in the hyperaccumulator.

DIFS modelling showed that the rhizosphere of the excluder was able to sustain the initially lower soluble Ni concentration through replenishment from the solid phase, while Ni resupply in the rhizosphere of the hyperaccumulator was not sufficient to maintain the initially high concentrations of soluble Ni. However, the amount of DGT-labile Ni was higher in the rhizosphere of the hyperaccumulator compared to the excluder in all deployment times.

Conclusion

Our data suggest that compounds derived from root activity, in particular DOC, are important controls of Ni availability to plants growing on serpentine soil.

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Acknowledgments

In memory of Petra S. Kidd (1972-2020†). Her dedication, enthusiasm and generosity were an essential contribution to this work. Her passion for the science will always be an inspiration to us. Vanessa Álvarez-López also thanks Christoph Hoefer and Andreas Kreuzeder for their help and company in the laboratory. This research was supported by the Spanish Ministerio de Economía y Competitividad (CTM2012-39904-C02-01) and FEDER, and by the 7th Framework Program of the European Commission (FP7-KBBE-266124, GREENLAND).

Funding

Spanish Ministerio de Economía y Competitividad (CTM2012-39904-C02-01) and FEDER, and the 7th Framework Program of the European Commission (FP7-KBBE-266124, GREENLAND.

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Author notes

  1. V. Álvarez-López

    Present address: Department of Crop Production, University of Santiago de Compostela (USC), Santiago, Spain

Authors and Affiliations

  1. Instituto de Investigacións Agrobiolóxicas de Galicia (IIAG), Consejo Superior de Investigaciones Científicas (CSIC), Santiago de Compostela, Spain

    V. Álvarez-López, Á. Prieto-Fernández & P.S. Kidd

  2. Department of Forest and Soil Sciences, Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), A-3430, Tulln, Austria

    V. Álvarez-López, M. Puschenreiter, J. Santner & W.W. Wenzel

  3. Department of Crop Sciences, Institute of Agronomy, University of Natural Resources and Life Sciences (BOKU), A-3430, Tulln, Austria

    J. Santner

  4. Faculty of Agriculture and Life Sciences, Lincoln University, Post Office Box 84, Lincoln, 7647, New Zealand

    N. Lehto

  5. Department of Soil Science and and Agricultural Chemistry, University of Santiago de Compostela (USC, Santiago, Spain

    C. Monterroso

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  1. V. Álvarez-López
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Álvarez-López, V., Puschenreiter, M., Santner, J. et al. Evidence for nickel mobilisation in rhizosphere soils of Ni hyperaccumulator Odontarrhena serpyllifolia. Plant Soil 464, 89–107 (2021). https://doi.org/10.1007/s11104-021-04944-7

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