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Pseudomonas citronellolis strain SLP6 enhances the phytoremediation efficiency of Helianthus annuus in copper contaminated soils under salinity stress

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Abstract

Background and aims

Plant growth-promoting bacteria (PGPB)-assisted phytoremediation, is a promising technique for the removal of copper (Cu) from contaminated soils. Moreover, soil salinity is considered to be one of the major threats to plant growth, and may therefore affect the progress and impact of the plant-driven phytoremediation. In this regard, the multiple interactions among Cu-salinity-plant-microbe, remain largely unknown. This study aimed to investigate the potential of PGPB on phytostabilization of Cu contaminated soils using Helianthus annuus growing under salinity stress.

Methods

A pot experiment was carried out to determine the effects of Cu and salinity tolerant PGPB inoculation in the phytoremediation of Cu-spiked soils by H. annuus under salt-stressed and non-stressed conditions.

Results

Cu- and salinity-tolerant bacterial strains were isolated from Cu contaminated soils. Out of the eight bacterial isolates, SLP6 was selected and identified as Pseudomonas citronellolis by 16S rRNA and characterized in terms of its Cu resistance (up to 400 mg L−1), salinity tolerance (up to 8% NaCl, w/v) and plant growth promoting (PGP) traits. Strain SLP6 displayed 1-aminocyclopropane-1-carboxylic acid deaminase activity, indole-3-acetic acid production, siderophores production, and phosphate solubilization under normal, Cu (100–400 mg L−1) and NaCl (2–8%, w/v) stressed conditions. The inoculation of H. annuus seeds with SLP6 strain significantly enhanced the plant growth, chlorophyll content, anti-oxidant enzymes production and Cu accumulation potential under Cu contamination with and without salt stress, whereas they reduced lipid peroxidation.

Conclusions

Pseudomonas citronellolis strain SLP6 could be used as a bio-inoculant for improving the phytostabilization of Cu-contaminated saline soils.

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Acknowledgements

S. Silambarasan thankfully acknowledges the financial support received from “Vicerrectoría de Investigación y Postgrado, Universidad de La Frontera, Chile”. P. Cornejo acknowledges the Agencia Nacional de Investigación y Desarrollo (ANID), Government of Chile for the grants: ANID/FONDECYT/1170264, ANID/FONDAP/15130015 and ANID/PIA/ACM170002.

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Authors and Affiliations

  1. Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile

    Sivagnanam Silambarasan, Peter Logeswari & Pablo Cornejo

  2. Botany and Zoology Department, Faculty of Science, University of Stellenbosch, Stellenbosch, South Africa

    Alexander Valentine

  3. Scientific and Technological Bioresource Nucleus, BIOREN-UFRO, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile

    Pablo Cornejo

  4. Rhizosphere Biology Laboratory, Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Velu Rajesh Kannan

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  1. Sivagnanam Silambarasan
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  2. Peter Logeswari
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  3. Alexander Valentine
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  4. Pablo Cornejo
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  5. Velu Rajesh Kannan
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Correspondence to Sivagnanam Silambarasan or Pablo Cornejo.

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Silambarasan, S., Logeswari, P., Valentine, A. et al. Pseudomonas citronellolis strain SLP6 enhances the phytoremediation efficiency of Helianthus annuus in copper contaminated soils under salinity stress. Plant Soil 457, 241–253 (2020). https://doi.org/10.1007/s11104-020-04734-7

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