Skip to main content
SpringerLink
Log in

Inoculations with Pseudomonas fluorescens and Bacillus cereus affect the soil enzyme activity, growth and rhizosphere microbial diversity of Taxus chinensis var. mairei

  • Regular Article
  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Background and aims

Taxus chinensis var. mairei is a rare tree endemic to China. Pseudomonas fluorescens CLW17 and Bacillus cereus CLY07 were isolated from the T. chinensis var. mairei rhizosphere and the two strains were efficient phosphate-solubilizing bacteria. We investigated the effect of inoculation with P. fluorescens CLW17 and B. cereus CLY07 on soil enzyme activity, growth and the rhizosphere microbial diversity of Taxus chinensis var. mairei over different time periods.

Methods

The plant biomass, seedling height, stem diameter and root vitality were measured, so that the inoculation effects of the two bacterial strains on T. chinensis var. mairei seedlings could be evaluated. Three types of soil enzymes (alkaline phosphatase, invertase and dehydrogenase) were identified. The soil microbial community composition and diversity were determined by high-throughput pyrosequencing analysis.

Results

Inoculation with P. fluorescens CLW17 and B. cereus CLY07 significantly increased plant biomass, seedling height, stem diameter and root vitality, and improved soil enzyme activities (alkaline phosphatase, invertase and dehydrogenase) (P < 0.05) in T. chinensis var. mairei. High-throughput pyrosequencing of 16S and 18S rRNA genes revealed minor changes in the diversity, relative abundance, and composition of the microbial community in the T. chinensis var. mairei rhizosphere after inoculation.

Conclusion

Inoculation with P. fluorescens CLW17 and B. cereus CLY07 promoted T. chinensis var. mairei growth and improved the soil enzyme activity with a small change in rhizosphere microbial diversity. This study provides necessary insight for developing effective, cheap and safe inoculants of strains P. fluorescens CLW17 and B. cereus CLY07 for T. chinensis var. mairei.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Adesemoye AO, Kloepper JW (2009) Plant-microbe interactions in enhanced fertilizer-use efficiency. Applied Microbiology & Biotechnology 85:1–12

    Article  CAS  Google Scholar 

  • Antonious GF (2003) Impact of soil management and two botanical insecticides on urease and Invertase activity journal of environmental science and health. Part B 38(4):479–488

    Google Scholar 

  • Banerjee S, Kirkby CA, Schmutter D, Bissett A, Kirkegaard JA, Richardson AE (2016) Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biology & Biochemistry 97:188–198

    Article  CAS  Google Scholar 

  • Brooks DD, Twieg BD, Grayston SJ, Jones MD (2013) Physical extent, frequency, and intensity of phosphatase activity varies on soil profiles across a Douglas-fir chronosequence. Soil Biol Biochem 64:1–8

    Article  CAS  Google Scholar 

  • Cao S, Liang QW, Nzabanita C, Li YZ (2019) Paraphoma root rot of alfalfa (Medicago sativa L.) in Inner Mongolia. China Plant Pathology 69(2):231–239

    Article  Google Scholar 

  • Chaurasia B, Pandey A, Palni L (2005) Distribution, colonization and diversity of arbuscular mycorrhizal fungi associated with central Himalayan rhododendrons. Forest Ecology & Management 207(3):315–324

    Article  Google Scholar 

  • Casida LE, Klein DA, Santro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376

    Article  CAS  Google Scholar 

  • Dorjey S, Dolkar D, Sharma R (2017) Plant growth promoting rhizobacteria Pseudomonas: a review. International Journal of Current Microbiology and Applied Sciences 6(7):1335–1344

    Article  CAS  Google Scholar 

  • Fan X, Hu H, Huang G, Huang F, Li Y, Paltapaz J (2015) Soil inoculation with Burkholderia sp. LD-11 has positive effect on water-use efficiency in inbred lines of maize. Plant & Soil 390:337–349

    Article  CAS  Google Scholar 

  • Frankenberger TW, Johanson BJ (1983) Method of measuring invertase activity in soil. Plant and Soil 74:301–311

    Article  CAS  Google Scholar 

  • Furtak K, Gałązka A (2019) Enzymatic activity as a popular parameter used to determine the quality of the soil environment. Polish Journal of Agronomy 37:22–30

    Google Scholar 

  • Gu Y, Wang P, Kong CH (2009) Urease, invertase, dehydrogenase and polyphenoloxidase activities in paddy soil influenced by allelopathic rice variety. Eur J soil biol 45:436–441

    Article  CAS  Google Scholar 

  • Hao DC, Song SM, Mu J, Hu WL, Xiao PG (2016) Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization. Scientific Reports 6:22006

    Article  CAS  Google Scholar 

  • Jha CK, Saraf M (2015) Plant growth promoting Rhizobacteria (PGPR): a review. Journal of Agricultural Research and Development 5(2):0108–0119

    Google Scholar 

  • Jorquera MA, Shaharoona B, Nadeem SM, de la Luz Mora M, Crowley DE (2012) Plant growth-promoting Rhizobacteria associated with ancient clones of creosote bush (Larrea tridentata). Microb Ecol 64:1008–1017

    Article  Google Scholar 

  • Jungwook Y, Kloepper JW, Choong-Min R (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science 14:1–4

    Article  Google Scholar 

  • Kotroczó Z, Veres Z, Fekete I, Krakomperger Z, Tóth JA, Lajth K, Tóthmétész B (2014) Soil enzyme activity in response to long-term organic matter manipulation. Soil Biology & Biochemistry 70:237–243

    Article  Google Scholar 

  • Kumar S, Chaudhuri S, Maiti SK (2013) Soil Dehydrogenase Enzyme Activity in Natural and Mine Soil - A Review Middle-East. Journal of Scientific Research 13:898–906

    CAS  Google Scholar 

  • Kumar A, Maurya BR, Raghuwanshi R, Meena VS, Islam MT (2016) Co-inoculation with Enterobacter and Rhizobacteria on Yield and Nutrient Uptake by Wheat (Triticum aestivum L.) in the Alluvial Soil Under Indo-Gangetic Plain of India Journal of Plant Growth Regulation:1–10

  • Li F, Chen L, Redmile-Gordon M, Zhang JB, Zhang CZ, Ning Q, Li W (2018) Mortierella elongata's roles in organic agriculture and crop growth promotion in a mineral soil Land Degrad Dev 1–10

  • Lugtenberg B, Kamilova F (2009) Plant-Growth-Promoting Rhizobacteria. Annual Review of Microbiology 63:541–556

    Article  CAS  Google Scholar 

  • Luo H, Tao YQ, Fan XY, Oh SK, Lu HX, Deng JX (2018) Identification and Characterization of Alternaria iridiaustralis Causing Leaf Spot on Iris ensata in China. Mycobiology 46(2):168–171

    Article  Google Scholar 

  • Majumder SP, Das AC (2016) Phosphate-solubility and phosphatase activity in Gangetic alluvial soil as influenced by organophosphate insecticide residues. Ecotoxicology and Environmental Safety 126:56–61

    Article  CAS  Google Scholar 

  • Pérez-Montaño F, Alías-Villegas C, Bellogín RA, del Cerro P, Espuny MR, Jiménez-Guerrero I, López-Baena FJ, Ollero FJ, Cubo T (2014) Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production. Microbiol Res 169:325–336

    Article  Google Scholar 

  • Pii Y, Mimmo T, Tomasi N, Terzano R, Cesco S, Crecchio C (2015) Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review Biology and Fertility of Soils 51:403–415

    Article  CAS  Google Scholar 

  • Prashar P, Kapoor N, Sachdeva S (2014) Rhizosphere: its structure, bacterial diversity and significance. Rev Environ Sci Biotechnol 13:63–77

    Article  CAS  Google Scholar 

  • Qian X, Gu J, Sun W, Li Y, Fu Q, Wang X, Gao H (2014) Changes in the soil nutrient levels, enzyme activities, microbial community function, and structure during apple orchard maturation. Applied soil ecology 77:18–25

    Article  Google Scholar 

  • R Core Team (2014) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/

  • Rawat SR, Mannisto MK, Bromberg Y, Haggblom MM (2012) Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils. FEMS Microbiology and Ecology 82:341e355

    Article  Google Scholar 

  • Ren JH, Zhang JF, Liu RX, Li YQ (2008) Study on Arbuscular Mycorrhizae in Taxus chinensis var. mairei. Acta Botanica Boreal Occidentalia Stinica 28(7):1468–1473 (in Chinese)

    Google Scholar 

  • Ren JH, Liu H, Wu XH, Wang Q, Ren YQ, Liu YJ, Feng YL (2012) Screening, identification and promoting effect of phosphate-solubilizing bacteria in rhizosphere of Taxus chinensis var. mairei. Acta Microbiologica Sinica 52(3):295–303 (in Chinese)

    CAS  PubMed  Google Scholar 

  • Roberts DW, St Leger RJ (2004) Metarhizium spp. Cosmopolitan Insect-Pathogenic Fungi: Mycological Aspects Advances in Applied Microbiology 54:1–70

    CAS  PubMed  Google Scholar 

  • Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances 17:319–339

    Article  CAS  Google Scholar 

  • Saia S, Rappa V, Ruisi P, Abenavoil RM, Sunnseri F, Giambalvo D, Frenda AS, Martinelli F (2015) Soil inoculation with symbiotic microorganisms promotes plant growth and nutrient transporter genes expression in durum wheat. Frontiers in Plant Science 6:1–10

    Article  Google Scholar 

  • Sylvia KEI, Sophie ZBWW (2010) Molecular diversity of fungal communities in agricultural soils from Lower Austria. Fungal diversity 44(1):65–75

    Article  Google Scholar 

  • Tabatabai M, Bremner J (1969) Use of p-nitrophenol phosphate in assay of soil phosphatase activity. Soil Biol Biochem 1:301–307

    Article  CAS  Google Scholar 

  • Tayung K, Jha DK, Deka DC (2007) Isolation and identification of antimicrobial agent-producing bacterium from Taxus baccata rhizosphere antagonistic against clinically significant microbes. Indian Journal of Microbiology 47(4):317–322

    Article  CAS  Google Scholar 

  • Vassilev N, Vassileva M, Nicolaeva I (2006) Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends. Applied Microbiology and Biotechnology 71:137–144

    Article  CAS  Google Scholar 

  • Verbon EH, Liberman LM (2016) Beneficial Microbes Affect Endogenous Mechanisms Controlling Root Development. Trends in Plant Science 21(3):218–229

    Article  CAS  Google Scholar 

  • White LJ, Ge X, Brözel VS, Subramanian S (2017) Root isoflavonoids and hairy root transformation influence key bacterial taxa in the soybean rhizosphere. Environ Microbiol 19:1391–1406

    Article  CAS  Google Scholar 

  • Wu LS, Hu CL, Han T, Zheng CJ, Ma XQ, Rahman K, Qin LP (2013) Cytotoxic metabolites from Perenniporia tephropora, an endophytic fungus from Taxus chinensis var. mairei. Applied microbiology and biotechnology 97:305–315

    Article  CAS  Google Scholar 

  • Xie W, Hao ZP, Yu M,Wu ZX, Zhao AH, Li JL, Zhang X, Chen BD (2018) Improved phosphorus nutrition by arbuscular mycorrhizal symbiosis as a key factor facilitating glycyrrhizin and liquiritin accumulation in Glycyrrhiza uralensis plant and soil 1–15

  • Xu LX, Yi M, Yi HL, Guo EH, Zhang AY (2018) Manure and mineral fertilization change enzyme activity and bacterial community in millet rhizosphere soils. World Journal of Microbiology and Biotechnology 34:8–21

    Article  CAS  Google Scholar 

  • Zhang Q, Liu HW, Sun GL, Wilson LW, Wu JQ, Hoffman A, Cheng JW, Qiu DY (2015) Baseline survey of root-associated microbes of Taxus chinensis (Pilger) Rehd Plos one 1–13

  • Zhang Y, Gao X, Shen Z, Zhu C, Jiao Z, Li R, Shen Q (2019) Pre-colonization of pgpr triggers rhizosphere microbiota succession associated with crop yield enhancement. Plant and Soil 439:553–567

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31100471), the Fund for Shanxi “1331 Project” Key Subjects Construction (1331KSC) and the Fund for Shanxi Key Subjects Construction (FSKSC), Key research and development program in Shanxi (No. 2016ZD0193). We thanked International Science Editing for editing this manuscript.

Author information

Authors and Affiliations

  1. The Department of Biological Science and Technology, Changzhi University, Changzhi, 046011, Shanxi, China

    Bianxia Bai, Xin Yang, Qingsong Zhao, Ruixiang Liu & Jiahong Ren

  2. Ecological and Environmental Research Institute of Taihang Mountain, Changzhi, 046011, Shanxi, China

    Bianxia Bai, Xin Yang, Qingsong Zhao, Ruixiang Liu & Jiahong Ren

Authors
  1. Bianxia Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qingsong Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruixiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiahong Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

BB, JR and XY designed and performed the experiments. BB and RL analyzed the data and drafted the manuscript. JR, BB, QZ and XY participated in revision of the manuscript. JR and BB finalized the manuscript.

Corresponding author

Correspondence to Jiahong Ren.

Ethics declarations

Conflict of interest

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Informed consent

We declare that we consent to submit the manuscript to Plant and Soil.

Human participants and/or animals

The authors declare that this study does not involve any human participants and animals.

Additional information

Responsible Editor: Hans Lambers.

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Supplementary Table 1

Bacterial and fungal taxonomy and abundance of all OTUs (XLS 505 kb)

ESM 1

(DOCX 1713 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bai, B., Yang, X., Zhao, Q. et al. Inoculations with Pseudomonas fluorescens and Bacillus cereus affect the soil enzyme activity, growth and rhizosphere microbial diversity of Taxus chinensis var. mairei. Plant Soil 455, 41–52 (2020). https://doi.org/10.1007/s11104-020-04660-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11104-020-04660-8

Keywords

Search

Navigation