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Rhizoglomus intraradices and associated Brevibacterium frigoritolerans enhance the competitive growth of Flaveria bidentis

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Abstract

Aims

Our aim was to determine whether there is a synergistic interaction between the arbuscular mycorrhizal (AM) fungus Rhizoglomus intraradices and the bacterium Brevibacterium frigoritolerans, and to understand how R. intraradices and B. frigoritolerans affect the competitive growth of Flaveria bidentis (L.) Kuntze in nutrient-deficient soil.

Methods

Pot culture of F. bidentis and Eclipta prostrata (L.) L. under nutrient-limited soil conditions was used to test the effect of R. intraradices and B. frigoritolerans on the competitive growth of F. bidentis.

Results

Inoculation with B. frigoritolerans and co-inoculation with R. intraradices and B. frigoritolerans increased the biomass of F. bidentis, and a single inoculation of B. frigoritolerans gave E. prostrata a competitive advantage over F. bidentis. However, we found higher R. intraradices colonization of exotic F. bidentis roots than native E. prostrata roots.

Conclusions

R. intraradices and B. frigoritolerans probably play different roles in the invasion of F. bidentis. R. intraradices may give the invader a competitive advantage through the accumulation of higher foliar levels of N, and B. frigoritolerans may facilitate the uptake of available N from the soil. Therefore, the results support the hypothesis that the AM fungus-soil bacteria cooperation makes N available to F. bidentis and facilitates its competitive growth.

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Abbreviations

AM:

Arbuscular mycorrhizal

ANOVA:

Analysis of variance

BF:

inoculated with Brevibacterium frigoritolerans

CK:

uninoculated treatment used as the control

RI:

inoculated with Rhizoglomus intraradices

CAAS:

Chinese Academy of Agricultural Science

CRCI:

Corrected index of relative competition intensity

PCA:

principal component analysis

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Acknowledgements

This research was funded by the National Natural Science Foundation of China (Grant No. 31972343), and Hebei National Natural Science Foundation (C2019201059). We thank Prof. Runjin Liu, Qingdao Agricultural University for providing the inoculum of Rhizoglomus intraradices.

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Author notes

  1. Ewei Du and Xue Chen contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Hebei University, Baoding, 071002, Hebei, China

    Ewei Du, Xue Chen, Haiyun Xu & Fengjuan Zhang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Qiao Li

  3. College of Economy, Hebei University, Baoding, 071002, Hebei, China

    Fengxin Chen

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  1. Ewei Du
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Correspondence to Haiyun Xu or Fengjuan Zhang.

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Du, E., Chen, X., Li, Q. et al. Rhizoglomus intraradices and associated Brevibacterium frigoritolerans enhance the competitive growth of Flaveria bidentis. Plant Soil 453, 281–295 (2020). https://doi.org/10.1007/s11104-020-04594-1

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