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Optimizing root architecture and increasing transporter gene expression are strategies to promote selenium uptake by high-se accumulating rice cultivar

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Abstract

Aim

Selenium uptake capacity can vary among rice genotypes; however, the processes driving these high- and low-Se-accumulating rice cultivars have not yet been defined.

Methods

The root architecture, selenium uptake, and transporter genes (Sultr2;1 and Ospt2) and selenium-binding protein (SBP1) expression of high- and low-Se rice cultivars were determined in agar culture experiment with different selenite and selenate concentrations.

Results

Root architecture analysis showed that the toxicity of selenite was greater than that of selenate for both rice cultivars, and the Se tolerance of the high-Se rice for selenite was greater than that of the low-Se rice. The uptake rate of selenite was greater than that of selenate, and the high-Se rice accumulated a higher Se concentration in the roots. However, selenite translocation via the xylem was limited, and the two rice cultivars accumulated similar amounts of Se in shoots when it was supplied as selenite. By contrast, selenate was highly mobile via xylem transport, and the high-Se rice accumulated a higher Se concentration in the shoots when it was supplied. The transcript levels of the Sultr2;1 and Ospt2 genes were higher in the high-Se rice than in the low-Se rice with selenite or selenate application. The application of selenite or selenate increased the SBP1 transcript level in the high-Se rice but decreased the transcript level of SBP1 in the low-Se rice.

Conclusions

The increased expression of phosphate and sulfate transporters and improved root architecture may explain how the high-Se rice cultivar can take up more selenite and selenate, respectively.

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Acknowledgements

We would like to thank Professor Martin Broadley (University of Nottingham, UK) for facilitating the analysis and for suggestions on the manuscript. This work was supported by the National Natural Science Funds of China (Grant No.31872176; 31501835), the Natural Science Foundation of Guangdong Province (Grant No.2015A030310449), the Science and Technology Project of Guangzhou (Grant No. 201804010341) and the President’s Special Fund of the Guangdong Academy of Agricultural Sciences (Grant No. 201938).

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Authors and Affiliations

  1. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Mu Zhang & Shuanhu Tang

  2. Key Laboratory of Plant Nutrition and Fertiliser in South Region, Ministry of Agriculture, Guangzhou, 510640, China

    Mu Zhang & Shuanhu Tang

  3. Division of Plant and Crop Science, Sutton Bonington Campus, University of Nottingham, Nottingham, LE12 5RD, UK

    Lolita Wilson & Linxi Jiang

  4. College of Agronomy, Shanxi Agricultural University, Taigu, 030801, China

    Guofang Xing

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  1. Mu Zhang
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  2. Lolita Wilson
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  3. Guofang Xing
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  5. Shuanhu Tang
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Correspondence to Shuanhu Tang.

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Zhang, M., Wilson, L., Xing, G. et al. Optimizing root architecture and increasing transporter gene expression are strategies to promote selenium uptake by high-se accumulating rice cultivar. Plant Soil 447, 319–332 (2020). https://doi.org/10.1007/s11104-019-04383-5

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