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Plant and Soil

, Volume 441, Issue 1–2, pp 613–627 | Cite as

High-resolution imaging of rhizosphere oxygen (O2) dynamics in Potamogeton crispus: effects of light, temperature and O2 content in overlying water

  • Chao Han
  • Jinghua Ren
  • Paul N. Williams
  • Fan Ke
  • Qiushi Shen
  • Zhaode WangEmail author
  • Di Xu
  • Jun LuoEmail author
Regular Article

Abstract

Aims

Radial oxygen loss (ROL) for macrophytes is intimately involved in their survival and growth, thus detailed characterizations of ROL and its implication for geochemical processes are of particular interest. We experimentally investigated ROL patterns from the submerged macrophyte species Potamogeton crispus and determined how light, temperature and O2 content in overlying water regulate O2 micro-distributions in the rhizosphere.

Method

Planar optodes were firstly used for non-destructive imaging of the O2 micro-distributions around single roots of P. crispus planted in rhizotrons containing sediment. The dynamic changes in below-ground O2 concentrations and oxygenation expansion at different light intensities (0–216 μmol photons m−2), temperatures (14 and 25 °C) and O2 content in overlying waters (0–256 μmoL−1) were quantified.

Results

P. crispus-mediated ROL is predominantly localized to the root apices with an average rate of 168.1 ± 21.4 nmol m−2 s−1 under nearly natural conditions (light, saturated overlying water at 14 °C), maintaining a visible oxygenated rhizosphere zone with a radius of 1.33 ± 0.21 mm. ROL is closely correlated with light intensity, suggesting photosynthetic O2 evolution. In darkness, the rhizosphere O2 availability is significantly reduced and strongly affected by the O2 content in overlying water, which passively diffuses into the plant leaves. Elevated temperatures lead to diminished ROL as a result of increased O2 demand of the surrounding sediment.

Conclusion

A high O2 microheterogeneity around P. crispus root apices is firstly demonstrated, and we provide direct empirical evidences that ROL are regulated dynamically by the shifting light, temperature and O2 content in overlying water.

Keywords

Freshwater macrophytes Planar optode imaging Radial oxygen loss (ROL) ROL-regulating factor Sediment oxygenation 

Notes

Funding

This work was supported by the National Science Foundation of China (No. 41773087) the Jiangsu Natural Science Foundation (Nos. BK20180109; BK20171075), and the Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences (SEPR2017–11).

Supplementary material

11104_2019_4150_MOESM1_ESM.docx (1.6 mb)
ESM 1 (DOCX 1636 kb)

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjingChina
  2. 2.Geological Survey of Jiangsu ProvinceNanjingChina
  3. 3.Institute for Global Food Security, School of Biological SciencesQueen’s University BelfastBelfastUK
  4. 4.State Key Laboratory of Pollution Control and Resource Reuse, School of the EnvironmentNanjing UniversityNanjingChina

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