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Coincidence of QTLs determining foliar phosphorus fractionation patterns and phosphorus utilization efficiency in barley under low phosphorus stress

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Abstract

Aims

Plants can optimize the allocation of phosphorus (P) among their foliar P fractions to increase the P utilization efficiency (PUE). Identifying the genetic relationships between foliar P fractionation and PUE could provide opportunities to improve the P efficiency of barley (Hordeum vulgare L.).

Methods

The differences in the concentrations and proportions of inorganic P, ester P, nucleic acid P and insoluble P between a wild barley cultivar CN4027 and a commercial cultivar Baudin were studied, and their quantitative trait loci (QTLs) at normal P (NP) and low P (LP) fertilisations were mapped. The PUE that was determined in the previous study was used to analyze the relationship between PUE and foliar P fractionation in this research.

Results

Both cultivars of barley could increase their metabolic P fractions as an LP stress tolerance strategy to ensure their continued metabolic activities in response to LP stress. Cultivar CN4027 showed higher nucleic acid P concentration (NPC), nucleic acid P proportion (NPP) and insoluble P proportion (IPP) than cultivar Baudin under LP stress. This abundant organic P (Po) pool of CN4027 ensured the normal functioning of its metabolic pathways under LP stress. The close relationships between the foliar P fractionation and PUE could be explained by two QTL clusters, Cl-3H.02 and Cl-5H.01. The QTL cluster Cl-3H.02 is flanked by the markers bPb3256099-bPb3255630 on chromosome 3H and controls the ester P concentration (EPC), ester P proportion (EPP), insoluble P concentration (IPC) and IPP.

Conclusions

The QTL cluster Cl-3H.02 might have great potential for the future genetic improvement of barley PUE and may offer clues for the genetic relationships between the foliar P fractionation and PUE.

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Abbreviations

P:

phosphorus

PUE:

phosphorus utilization efficiency

LP:

low P

NP:

normal P

QTL:

quantitative trait loci

MAS:

marker-assisted selection

RILs:

recombinant inbred lines

PAE:

P acquisition efficiency

Pi:

inorganic P

Po:

organic P

PiC:

inorganic P concentration

EPC:

ester P concentration

NPC:

nucleic acid P concentration

IPC:

insoluble P concentration

PiP:

inorganic P proportion

EPP:

ester P proportion

NPP:

nucleic acid P proportion

IPP:

insoluble P proportion.

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Acknowledgements

This work was supported by the National Natural Science Foundation (31401377), Science and Technology Project of Sichuan Province (2014NZ0008; 2017JY0126) and Key Project of Education Department of Sichuan Province (14ZA0002).

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Author notes

  1. Jingyi Guo and Daihua Ye contributed equally to this work.

Authors and Affiliations

  1. College of Resources, Sichuan Agricultural University, Chengdu, 611130, China

    Guangdeng Chen, Haiying Yu, Xizhou Zhang & Tingxuan Li

Authors
  1. Jingyi Guo
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  2. Daihua Ye
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  3. Guangdeng Chen
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  4. Haiying Yu
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  5. Xizhou Zhang
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  6. Tingxuan Li
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Correspondence to Xizhou Zhang or Tingxuan Li.

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Responsible Editor: Hans Lambers.

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Highlights

• The wild barley cultivar CN4027 showed a stronger organic P pool to improve P utilization efficiency (PUE) than the commercial barley cultivar Baudin.

• The relationships between foliar P fractionations and PUE could be explained by the coincidence of quantitative trait loci (QTLs) for these traits.

• The QTL cluster Cl-3H.02 could have great potential for future genetic improvement of P efficiency in barley.

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Guo, J., Ye, D., Chen, G. et al. Coincidence of QTLs determining foliar phosphorus fractionation patterns and phosphorus utilization efficiency in barley under low phosphorus stress. Plant Soil 441, 349–362 (2019). https://doi.org/10.1007/s11104-019-04128-4

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  • DOI: https://doi.org/10.1007/s11104-019-04128-4

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