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Plant and Soil

, Volume 438, Issue 1–2, pp 421–433 | Cite as

Characterizing differences in microbial community composition and function between Fusarium wilt diseased and healthy soils under watermelon cultivation

  • Tingting Wang
  • Yuewen Hao
  • Mingzhu Zhu
  • Sitian Yu
  • Wei Ran
  • Chao XueEmail author
  • Ning Ling
  • Qirong Shen
Regular Article

Abstract

Aims

Continuous cropping of watermelon is known to result in the disruption of the rhizospheric bacteria and fungi that contribute to the occurrence of Fusarium wilt disease. However, the underlying changes in microbial composition and function as a response to mono-cropping are less studied.

Methods

In this study, differences in composition and potential function of the microbiome between healthy and diseased soils were investigated using MiSeq targeted sequencing and the functional GeoChip array, respectively.

Results

Twenty years of continuous watermelon monoculture was found to significantly alter the soil microbial communities by increasing bacterial diversity but decreasing fungal diversity. Compare to bacterial network, fungal co-occurrence networks were less robust and less connected in the monoculture diseased soil. Identified keystone species, belonging to the Proteobacteria, Bacteroidetesand Acidobacteria, were present in both the diseased and healthy soils. Key fungal species from the healthy soil belonged solely within the Ascomycete, while in the diseased soil Basidiomycota were dominant. As such, overall variations in the composition of the soil microbiome are accompanied by changes in the identities of the keystone species when comparing healthy versus diseased soils, further suggesting that soil function may also be altered. Relative abundances of genes associated with the degradation of hemicelluloses and chitin, the Calvin circle, ammonification, stress responses, iron uptake, and nitrogen fixation were significantly higher under long-term monoculture. Particularly, Fusarium spp. relative abundance was positively correlated with the relative abundances of genes involved in adherence, cellular metabolism, and immune evasion which may facilitate pathogen infection of plant roots.

Conclusions

In conclusion, these results highlight the significant compositional and functional differences in microbial communities between Fusarium wilt diseased soils and healthy soils under watermelon cultivation. This provides insight into the complex array of microorganisms in soils that suffer from Fusarium disease and illustrates potential directions towards the manipulation of the soil microbiome for suppression of this disease.

Keywords

Fusarium wilt of watermelon Microbial composition Microbial function Continuous monocropping GeoChip 

Notes

Acknowledgements

This study was supported by the National Nature Science Foundation of China (31772398), Special Fund for Agro-scientific Research in the public Interest (201503110), China Science and Technology Ministry (2015CB150500), the Jiangsu Science and Technology Department (BK20160730), and the China Postdoctoral Science Foundation (2017 M621761 and 2018 T110510), the Fundamental Research Funds for the Central Universities (KYZ201720).

Supplementary material

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Tingting Wang
    • 1
  • Yuewen Hao
    • 1
  • Mingzhu Zhu
    • 1
  • Sitian Yu
    • 1
  • Wei Ran
    • 1
  • Chao Xue
    • 1
    Email author
  • Ning Ling
    • 1
  • Qirong Shen
    • 1
  1. 1.Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNanjing Agricultural UniversityNanjingChina

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