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Mesorhizobium jarvisii sv. astragali as predominant microsymbiont for Astragalus sinicus L. in acidic soils, Xinyang, China

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Abstract

Aims

To identify the rhizobia nodulating Astragalus sinicus in the acid soils at Xinyang, China and to estimate the effects of fertilization strategies on the rhizobial community.

Methods

Soil samples were collected from treatments submitted to different fertilization strategies (chemical fertilizers, A. sinicus green manure, various input levels). Nodules obtained from the A. sinicus trapping plants grown in the soil samples were used for rhizobial isolation. The isolates were grouped into PCR-RFLP genotypes based on analyses of 16S rRNA gene and IGS, and representatives for each genotype were further subjected to phylogenetic analyses of housekeeping genes (16S rRNA gene, atpD, glnII, and rpoB), as well as symbiotic genes (nodC and nifH). Soil physicochemical characteristics were also estimated and redundancy analysis was performed to examine the relations between soil features and distribution of rhizobial genotypes across different fertilization treatments.

Results

A total of 257 rhizobial isolates were obtained and discriminated into 12 IGS types in a single 16S rRNA type by RFLP analyses. Further 16S rRNA gene sequence-based phylogeny and multilocus sequence analysis (MLSA) distinguished a minor group (10 strains, 4 IGS types) affiliated to Mesorhizobium huakuii and a main group (247 strains, 8 IGS types) to Mesorhizobium jarvisii, for which a novel symbiovar, sv. astragali, was described. The long-term input of A. sinicus green manure increased rhizobial diversity and modified rhizobial community structures, via the increase of organic matter and decrease of soil pH, available phosphate, available potassium, and total salt.

Conclusions

M. jarvisii sv. nov. astragali was the predominant microsymbiont of A. sinicus in the tested acidic soils. The long-term input of green manure modified the soil features, enhancing the genetic diversity of rhizobia associated with A. sinicus and alternating the rhizobial community structure.

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Funding

This work was financed by the China Agriculture Research System—Green Manure (Project No. CARS-22), the National Natural Science Foundation of China (Project No. 31400008 and 31571778), National Human and Social Security Ministry of Science and Technology for Overseas Returnees (Project No. 21104000005). ETW is financially supported by grants SIP20171259 authorized by Instituto Politécnico Nacional, Mexico.

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Authors and Affiliations

  1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Junjie Zhang, Yimin Shang, Chen Guo, Xu Yang & Jianqiang Zheng

  2. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Junjie Zhang

  3. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou, 450002, Henan Province, People’s Republic of China

    Junjie Zhang

  4. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, México, D. F., Mexico

    Entao Wang

  5. State Key Laboratory of Agrobiotechnology, Beijing, 100193, People’s Republic of China

    Wenfeng Chen

  6. College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing, 100193, People’s Republic of China

    Wenfeng Chen

  7. IRD, LSTM, Campus de Baillarguet TA-A-82/J, 34398, Montpellier Cedex 5, France

    Philippe de Lajudie

  8. Institute of Plant Nutrition and Resource Environment, Henan Academy of Agricultural Sciences, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Benyin Li & Chunzeng Liu

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  1. Junjie Zhang
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  2. Yimin Shang
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  9. Jianqiang Zheng
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  10. Chunzeng Liu
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Correspondence to Junjie Zhang or Chunzeng Liu.

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Zhang, J., Shang, Y., Wang, E. et al. Mesorhizobium jarvisii sv. astragali as predominant microsymbiont for Astragalus sinicus L. in acidic soils, Xinyang, China. Plant Soil 433, 201–212 (2018). https://doi.org/10.1007/s11104-018-3830-3

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