Impact of local forest composition on soil fungal communities in a mixed boreal forest

Plant and Soil volume 432, pages345–357(2018)Cite this article

Abstract

Aims

While fungi are key drivers of the carbon cycle and obligate symbionts of trees, the link between plant-fungal interactions and landscape vegetation changes has been largely overlooked. Our aim was to test whether a local difference in dominant tree species would shape the composition of soil fungi communities.

Methods

Fungal communities were described using next-generation DNA sequencing. Composite soil samples were collected in four paired sites (represented by one pure aspen stand and one pure spruce stand) and soil nutriments were measured.

Results

Of the more than 1119 OTUs, 31.6% were Ascomycota while 27.8% were Basidiomycota, 15% were ectomycorrhizal fungi whereas 19.7% were saprotrophic. Communities displayed high species turnover among forest types rather than differences in species richness. Among tested predictors, the dominant tree species explained around 11% of fungal community variation. pH and soil nutrients were also strong predictors of fungal communities.

Conclusions

Our study revealed strong correlations between dominant tree species and fungal communities at a local scale and raised questions regarding the impact of fungal communities on forest soil nutrient dynamics.

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Acknowledgements

The authors sincerely thank Evick Mestre, Danielle Charon and Raynald Julien for their assistance in the field, Francine Tremblay for laboratory access, Lucie Zinger for assistance with bioinformatics and sequence analyses, Benjamin Durrington for editing the text and WFJ Parsons for English revision. We are grateful to our internal reviewer, Julien Demenois (CIRAD), for helpful advice and comments on a previous version of the manuscript. We are grateful to the Genotoul Bioinformatics Platform, Toulouse Midi-Pyrenees, for providing computing and storage resources. We also thank two anonymous reviewers and section editor Thomas W. Kuyper for their relevant and helpful comments on previous versions of the manuscript.

Funding

This work was supported by Mitacs Acceleration in collaboration with Norbord Inc. [IT066831], the UQAT-UQAM-NSERC Chair in sustainable forest management, the French Laboratory of Excellence project “TULIP” (ANR-10-LABX-41; ANR-11-IDEX-0002-02), and University Paul Sabatier for travel fellowships.

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  1. Laboratoire Evolution et Diversité Biologique, UMR5174, Université Paul Sabatier – CNRS, 118 route de Narbonne, F-31062, Toulouse cedex, France

    Mélissande Nagati, Mélanie Roy, Sophie Manzi & Monique Gardes

  2. Chaire industrielle UQAM-UQAT en aménagement forestier durable, Institut de recherche sur les forêts, Université du Québec en Abitibi-Témiscamingue, 445 Boul. de l’Université, Rouyn-Noranda, QC J9X 4E5, Canada

    Mélissande Nagati, Annie Desrochers & Yves Bergeron

  3. Centre d’Ecologie Fonctionnelle et Evolutive UMR5175, 119 route de Mende, F-34293, Montpellier cedex 5, France

    Franck Richard

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Supplementary Figure 1
figure4

Location of study sites. (PNG 310 kb)

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Supplementary Figure 2
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OTU accumulation curves of fungal communities from black spruce organic layer (red), black spruce mineral layer (blue), trembling aspen organic layer (orange) and trembling aspen mineral layer (green), vertical bars represent standard errors of the mean. (PNG 321 kb)

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Supplementary Figure 3
figure6

Number of OTUs by order of Ascomycota and by sample type (TA-M = trembling aspen mineral layer, TA-O = trembling aspen organic layer, BS-M = black spruce mineral layer and BS-O = black spruce organic layer). (PNG 2500 kb)

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Supplementary Figure 4
figure7

Number of OTUs by genera of Basidiomycota and by sample type (TA-M = trembling aspen mineral layer, TA-O = trembling aspen organic layer, BS-M = black spruce mineral layer and BS-O = black spruce organic layer). (PNG 2597 kb)

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Supplementary Figure 5
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Venn diagram representing shared fungal OTUs between black spruce organic layer (BS-O), black spruce mineral layer (BS-M), trembling aspen organic layer (TA-O) and trembling aspen mineral layer (TA-M). (PNG 92 kb)

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Nagati, M., Roy, M., Manzi, S. et al. Impact of local forest composition on soil fungal communities in a mixed boreal forest. Plant Soil 432, 345–357 (2018). https://doi.org/10.1007/s11104-018-3806-3

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Keywords

  • Black spruce-feather moss
  • Fungal diversity
  • Soil ecology
  • Picea mariana
  • Populus tremuloides
  • NGS