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Effects of shrub encroachment on vertical changes in soil organic carbon in Mongolian grasslands: using a multi-biomarker approach

Plant and Soil volume 431, pages 217–230 (2018)Cite this article

Abstract

Aims

In recent decades, ecologists have investigated the effects of shrub encroachment on regional carbon cycling in semi-arid and arid regions. Although differences in carbon sequestration and stocks have been recognized in different soil layers, the vertical changes in soil organic carbon (SOC) at the molecular level following shrub encroachment remain unexplored. In this study, we used biomarkers to assess the impacts of shrub encroachment on SOC composition.

Methods

We collected soil samples along the 1-m profile within shrub patches and grassy matrix in three typical shrub-encroached grassland (SEG) sites in Inner Mongolia, and quantified the vertical distribution of the biomarkers, including free lipids, bound lipids and lignin-derived phenols.

Results

The principal component analysis (PCA) of the biomarker data showed that samples in the grassy matrix had strong positive loadings along the first two components (PCs); in contrast, samples in the upper 50 cm of the shrub patches had negative loadings along the first component (PC1) as well as a narrower range. The acid to aldehyde ratios of the vanillyl and syringyl type monomers increased simultaneously along the 1-m profile in the grassy matrix; however, this trend was not observed in the shrub patches. In addition, the vanillyls to syringyls to cinnamyls ratio was approximately 3:2:1 in the shrub patches and 3:2:1 or 2:2:1 in the grassy matrix.

Conclusions

Shrub encroachment altered the vertical patterns in SOC composition, especially in the upper 50 cm, as well as the oxidation status of lignin-derived phenols along the entire 1-m profile. Further, shrub encroachment influenced the soil carbon composition under the shrub canopy as well as in the grassy matrix due to the sprawling canopies and the horizontal extension of the root systems of the encroaching shrubs. These results provided new insights into the vertical patterns of SOC changes after shrub encroachment at the molecular level and have important implications for understanding the mechanisms of soil carbon dynamics with changes in vegetation structure and composition.

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (31330012, 31470525), the National Basic Research Program of China on Global Change (2014CB954001), the Key Research Program of Frontier Sciences, CAS, (QYZDY-SSW-SMC011).

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Authors and Affiliations

  1. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Luhong Zhou, He Li, Haihua Shen, Aijun Xing, Yankun Zhu & Jingyun Fang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Luhong Zhou, Haihua Shen, Aijun Xing, Yankun Zhu & Jingyun Fang

  3. Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai, 201306, China

    Yunping Xu

  4. College of Urban and Environmental Sciences, and Key Laboratory of Earth Surface Processes of the Ministry of Education, Peking University, Beijing, 100871, China

    Yinghui Wang, Shangzhe Zhou & Jingyun Fang

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  1. Luhong Zhou
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  2. He Li
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  3. Haihua Shen
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Correspondence to Jingyun Fang.

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Zhou, L., Li, H., Shen, H. et al. Effects of shrub encroachment on vertical changes in soil organic carbon in Mongolian grasslands: using a multi-biomarker approach. Plant Soil 431, 217–230 (2018). https://doi.org/10.1007/s11104-018-3761-z

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  • DOI: https://doi.org/10.1007/s11104-018-3761-z

Keywords

  • Carbon cycling
  • Lignin
  • Plant-soil feedback
  • Principal component analysis
  • Shrub encroachment
  • Soil process