Effects of Bacillus amyloliquefaciens and different phosphorus sources on Maize plants as revealed by NMR and GC-MS based metabolomics

  • Giovanni Vinci
  • Vincenza Cozzolino
  • Pierluigi Mazzei
  • Hiarhi Monda
  • Davide Savy
  • Marios Drosos
  • Alessandro Piccolo
Regular Article
  • 30 Downloads

Abstract

Aims

Plant growth-promoting bacteria of the genus Bacillus are known to solubilize phosphates and enhance plant growth in many plant species. We explored the effects of the inoculation with a commercial isolate Bacillus amyloliquefaciens on the growth and metabolic processes of maize plants in pot soils treated with triple superphosphate, rock phosphate, and either cow- or horse-manure composts, as P-fertilizers.

Methods

The metabolic profiles of maize leaves in the different treatments were determined by both Gas Chromatography–Mass Spectrometry and Nuclear Magnetic Resonance spectroscopy. Principle Components Analysis (PCA) based on data matrix from both techniques revealed a relationship between treatments and specific plant metabolites.

Results

Inoculated plants showed larger P and N contents and a more differentiated metabolome when treated with the two composts than with inorganic fertilizers. B. amyloliquefaciens in combination with composts significantly increased glucose, fructose, alanine and GABA metabolites in maize leaves, thus suggesting an improved photosynthetic activity due to enhanced P and N uptake. Both composts sustained plant growth and the phosphate solubilizing activity of B. amyloliquefaciens, while differences in P and N contents in plant leaves were attributed to the different content in compost of lignin residues and alkyl moieties, and consequent impact on microbial growth.

Conclusions

The combination of B. amyloliquefaciens inoculation with composted organic P-fertilizers rich in available metabolic carbon appears as an efficient alternative to mineral fertilizers to enhance nutrients uptake and foster growth mechanisms in maize plants.

Keywords

Metabolomics Phosphate-solubilizing-bacteria Compost Rock phosphate Triple Superphosphate GC-MS 1H-NMR Thermochemolysis 

Notes

Acknowledgements

This work was conducted in partial fulfillment of first author PhD requirements, and received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under grant agreement n° 312117 (BIOFECTOR).

Supplementary material

11104_2018_3701_MOESM1_ESM.docx (249 kb)
ESM 1 (DOCX 249 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Giovanni Vinci
    • 1
  • Vincenza Cozzolino
    • 1
  • Pierluigi Mazzei
    • 1
  • Hiarhi Monda
    • 1
  • Davide Savy
    • 1
  • Marios Drosos
    • 1
  • Alessandro Piccolo
    • 1
  1. 1.Centro Interdipartimentale di Ricerca sulla Risonanza Magnetica Nucleare per l’Ambiente, l’Agroalimentare ed i Nuovi Materiali (CERMANU)Università di Napoli Federico IIPorticiItaly

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