Effects of Bacillus amyloliquefaciens and different phosphorus sources on Maize plants as revealed by NMR and GC-MS based metabolomics
Plant growth-promoting bacteria of the genus Bacillus are known to solubilize phosphates and enhance plant growth in many plant species. We explored the effects of the inoculation with a commercial isolate Bacillus amyloliquefaciens on the growth and metabolic processes of maize plants in pot soils treated with triple superphosphate, rock phosphate, and either cow- or horse-manure composts, as P-fertilizers.
The metabolic profiles of maize leaves in the different treatments were determined by both Gas Chromatography–Mass Spectrometry and Nuclear Magnetic Resonance spectroscopy. Principle Components Analysis (PCA) based on data matrix from both techniques revealed a relationship between treatments and specific plant metabolites.
Inoculated plants showed larger P and N contents and a more differentiated metabolome when treated with the two composts than with inorganic fertilizers. B. amyloliquefaciens in combination with composts significantly increased glucose, fructose, alanine and GABA metabolites in maize leaves, thus suggesting an improved photosynthetic activity due to enhanced P and N uptake. Both composts sustained plant growth and the phosphate solubilizing activity of B. amyloliquefaciens, while differences in P and N contents in plant leaves were attributed to the different content in compost of lignin residues and alkyl moieties, and consequent impact on microbial growth.
The combination of B. amyloliquefaciens inoculation with composted organic P-fertilizers rich in available metabolic carbon appears as an efficient alternative to mineral fertilizers to enhance nutrients uptake and foster growth mechanisms in maize plants.
KeywordsMetabolomics Phosphate-solubilizing-bacteria Compost Rock phosphate Triple Superphosphate GC-MS 1H-NMR Thermochemolysis
This work was conducted in partial fulfillment of first author PhD requirements, and received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under grant agreement n° 312117 (BIOFECTOR).
- Amiour N, Imbaud S, Clément G, Agier N, Zivy M, Valot B, Tercet-Laforgue T (2012) The use of metabolomics integrated with transcriptomic and proteomic studies for identifying key steps involved in the control of nitrogen metabolism in crops such as maize. J Exp Bot 63:5017–5033CrossRefPubMedGoogle Scholar
- Brady NC, Weil RR (2008) The nature and properties of soils (fourteenth ed.), Prentice Hall, Upper Saddle River, NJ, USAGoogle Scholar
- Cozzolino V, Di Meo V, Piccolo A (2013) Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. J Geochem Explor 129:40–44Google Scholar
- Cozzolino V, Di Meo V, Monda H, Spaccini R, Piccolo, A (2016) The molecular characteristics of compost affect plant growth, arbuscular mycorrhizal fungi, and soil microbial community composition. Biol Fert Soils 52:15–29Google Scholar
- Gericke S, Kurmies B (1952) Die kolorimetrische Phosphorsaurebestimmung mit Ammonium-Vanadat-Molybdat und ihre Anwendung in der Pflanzenanalyse. Z Pflanzenernähr Bodenkd 59:32–35Google Scholar
- Koliaei AA, Akbari Gh A, Armandpisheh O, Labbafi MR, Zarghami R (2011) Effects of phosphate chemical fertilizers and biologic fertilizers in various moisture regimes on some morphological characteristics and seeds performance in maize S.C.704. Asian Journal of Agriculture and Food Sciences 3:223–234Google Scholar
- Lekfeldt JDS, Rex M, Mercl F, Kulhánek M, Tlustoš P, Magid J, de Neergaard A (2016) Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat. Chem Biol Technol Agric 3:22Google Scholar
- Li M, Welti R, Wang X (2006) Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphorus starvation. Roles of phospholipases Dζ1 and Dζ2 in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphorus-starved plants. Plant Physiol 142:750–761CrossRefPubMedPubMedCentralGoogle Scholar
- Li M, Cozzolino V, Mazzei P, Drosos M, Monda H, Hu Z, Piccolo A (2017) Effects of microbial bioeffectors and P amendements on P forms in a maize cropped soil as evaluated by 31 P–NMR spectroscopy. Plant Soil:1–18Google Scholar
- Lichtenthaler HK (1987) Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. In: Douce R, Packer L (eds) Methods Enzymol. 148, 350–382. Academic Press Inc., New YorkGoogle Scholar
- Sengupta S, Mukherjee S, Basak P, Majumder AL (2015) Significance of galactinol and raffinose family oligosaccharide synthesis in plants. Front Plant Sci. 26 6:656Google Scholar
- Thonar C, Lekfeldt JDS, Cozzolino V, Kundel D, Kulhánek M, Mosimann C, Neumann G, Piccolo A, Rex M, Symanczik S, Walder F, Weinmann M, de Neergaard A, Mäder P (2017) Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils. Chem Biol Technol Agric 4:7. https://doi.org/10.1186/s40538-017-0088-6 CrossRefGoogle Scholar
- Zhao L, Hu J, Huang Y, Wang H, Adeleye A, Ortiz C, Keller A (2016) 1H NMR and GC MS based metabolomics reveal nano-Cu altered cucumber (Cucumis sativus) fruit nutritional supply. Plant Physiol Biochem. https://doi.org/10.1016/j.plaphy.2016.02.010