Plant and Soil

, Volume 426, Issue 1–2, pp 365–381 | Cite as

Genome-wide analysis and expression profiling of the HMA gene family in Brassica napus under cd stress

  • Nannan Li
  • Hua Xiao
  • Juanjuan Sun
  • Shufeng Wang
  • Jingchao Wang
  • Peng Chang
  • Xinbin Zhou
  • Bo Lei
  • Kun Lu
  • Feng Luo
  • Xiaojun Shi
  • Jiana Li
Regular Article


Background and aims

Brassica napus (B. napus), one of the most important oil crop species, is highly tolerant to and accumulates high amounts of cadmium (Cd). Many iron transporters in plants have been identified to be Cd transporters. For example, some members of the heavy metal P1B-ATPase transporter family are responsible for Cd translocation in various plant species and play a vital role in Cd detoxification. However, the Cd translocation mechanism in B. napus and the characterization of the heavy metal ATPase (HMA) in B. napus remain unknown.


B. napus plants were treated with 50 μM or 200 μM Cd in soil for 30 days during the initial flowering stage. The dry weight of the plants and the Cd contents within their various tissues were then measured, after which the RNA in the leaves was extracted for transcriptomic analysis and subsequent quantitative real-time PCR (qRT-PCR) assays. After all the significantly regulated iron transporters were screened in response to Cd stress, the BnHMA gene family was identified and shown to link BnHMA genes with Cd translocation in the leaves of B. napus.


The transcriptomic analysis of B. napus leaves in response to Cd treatment revealed that several HMA genes (BnHMA2;2, BnHMA2;3 and BnHMA2;5) respond to Cd stress. We further examined the whole HMA family in B. napus; 31 BnHMA genes were subsequently identified. Their expression levels in different tissues and stages as well as their phylogenetic tree, gene structure, chromosomal location, conserved motifs, 3D models and subcellular localization were analyzed. The results showed that these HMA genes exhibit typical characteristics of HMA genes. In addition, the qRT-PCR results showed that the BnHMA2;3 expression levels in the B. napus plants treated with 50 μM or 200 μM Cd were seven- and ninefold greater than those under Cd-free conditions, respectively. Additional yeast experiment assays verified that BnHMA2;3 can transport Cd.


BnHMA2;3 may play an important role in Cd translocation in the leaves of B. napus. The results of this study may provide direction and useful information for increased understanding of the Cd stress-response mechanism.


Cadmium Heavy metal ATPase Metal transporter Brassica napus 




B. napus

Brassica napus














Differentially expressed genes


Gene Ontology


Funding information

This work was supported by National Key R & D Program of China (2017YFD0200200- 2017YFD0200208),the National Natural Foundation of China (31,400,063; C150705) and Fundamental Research Funds for the Central Universities (XDJK2017B030; SWU116021)., Research Funds of Scientific Platform and Base Construction (cstc2014pt-sy0017), the Key Special Program of China National Tobacco Corporation (TS-02–20,110,014), the Key Laboratory Program of China National Tobacco Corporation (110201603009) and The Recruitment Program for Foreign Experts (No. WQ20125500073).

Compliance with ethical standards

Competing financial interests

The authors declare no competing financial interests.

Supplementary material

11104_2018_3637_MOESM1_ESM.docx (2.2 mb)
ESM 1 (DOCX 2228 kb)


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Nannan Li
    • 1
    • 2
  • Hua Xiao
    • 1
  • Juanjuan Sun
    • 1
  • Shufeng Wang
    • 1
  • Jingchao Wang
    • 1
  • Peng Chang
    • 1
  • Xinbin Zhou
    • 1
  • Bo Lei
    • 3
  • Kun Lu
    • 2
    • 4
  • Feng Luo
    • 1
  • Xiaojun Shi
    • 1
  • Jiana Li
    • 2
    • 3
  1. 1.College of Resources and EnvironmentSouthwest UniversityChongqingPeople’s Republic of China
  2. 2.Southwest UniversityAcademy of Agricultural ScienceChongqingPeople’s Republic of China
  3. 3.Upland Flue-Cured Tobacco Quality and Ecology Key Laboratory of China TobaccoGuizhou Academy of Tobacco ScienceGuiyangChina
  4. 4.College of Agronomy and BiotechnologySouthwest UniversityChongqingPeople’s Republic of China

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