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Volatile allelochemicals of Chenopodium ambrosioides L. induced mitochondrion-mediated Ca2+-dependent and Caspase-dependent apoptosis signaling pathways in receptor plant cells

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Abstract

Aims

This study aims to explain the mechanism of action of allelochemical effect of Chenopodium ambrosioides L. volatile oil on mitochondrial characteristics of receptor plants.

Methods

This study chose maize (Zea mays L.), which is widely grown in C. ambrosioides invaded field as the receptor plant, and investigated the allelochemical effect of C. ambrosioides volatile oil and its two main components (α-terpinene and cymene) on maize root tip cells using a suspended gas method and TdT-mediated dUTP Nick-End Labeling (TUNEL) assays. The allelochemical effect on the relative expression of the apoptosis-associated type II Metacaspase gene ZmMCII was measured by quantitative real time polymerase chain reaction (qRT-PCR). The allelochemical effect on mitochondrial characteristics was analyzed by staining assays.

Results

The results showed that applications of C. ambrosioides volatile oil, α-terpinene, and cymene on maize root tip cells caused membrane lipid peroxidation, increased mitochondrial H2O2 and MDA contents, decreased mitochondrial membrane potential, released mitochondrial Cyt c and Ca2+ into the cytoplasm, and increased cytosolic Ca2+ level and expression of the type II Metacaspase gene ZmMCII. These apoptotic effects were time-dependent.

Conclusions

It suggested that C. ambrosioides volatile allelochemicals induced accumulation of reactive oxygen species (ROS) and triggered mitochondrion-mediated Ca2+- and Caspase-dependent apoptosis signaling pathways in receptor plant cells.

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Acknowledgements

This project was financially supported by National Natural Science Foundation of China (Project No. 31370549) and Key Program for Basic Research of Sichuan Province (Project No. 2017JY0017).

Author contribution statement

All authors conceived and designed research. YH and BC conducted experiments. DM, YH, YW and BH contributed to data acquisition. DM, YH, YW and BH contributed to the analysis and interpretation of data. JL and DM wrote and revised the manuscript. All authors read and approved the manuscript.

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Authors and Affiliations

  1. College of Life Science, Sichuan Normal University, Chengdu, People’s Republic of China

    Jiao Li, Yaqiang He, Danwei Ma, Bing He, Yanan Wang & Bin Chen

Authors
  1. Jiao Li
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  2. Yaqiang He
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  3. Danwei Ma
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  4. Bing He
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  5. Yanan Wang
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  6. Bin Chen
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Correspondence to Danwei Ma.

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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Li, J., He, Y., Ma, D. et al. Volatile allelochemicals of Chenopodium ambrosioides L. induced mitochondrion-mediated Ca2+-dependent and Caspase-dependent apoptosis signaling pathways in receptor plant cells. Plant Soil 425, 297–308 (2018). https://doi.org/10.1007/s11104-018-3593-x

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