Plant and Soil

, Volume 425, Issue 1–2, pp 321–333 | Cite as

Effects of elevated ozone on the contribution of nitrogen rhizodeposition by spring wheat to different soil N pools

  • Yanhong Cao
  • Yi Shi
  • Xinchao Sun
  • Caiyan LuEmail author
  • Xueyan LiuEmail author
Regular Article


Background and aims

Elevated ozone (O3) decreases nitrogen derived from rhizodeposition (NdfR). However, the changes in the partitioning of NdfR in soil N pools due to O3 remain unclear. The aims of this study were to investigate the contribution of NdfR to different soil N pools and its response to elevated O3 conditions.


Spring wheat was labeled with 15N–urea using a split-root technique under ambient (~40 ppb) and elevated O3 treatments (60 ± 5 ppb and 110 ± 5 ppb) in open-top chambers. Mineral-N, microbial biomass (MB)-N and fixed ammonium (FA)-N in rhizospheric soils were analyzed.


N rhizodeposition contributed 12–33% of mineral N, 10–14% of FA-N and 6–16% of MB-N under ambient O3. Elevated O3 significantly decreased mineral-15NdfR and increased FA-15NdfR but had no significant influence on MB-15NdfR. The decrease in mineral-NdfR was likely due to the decrease in rhizodeposition inputs and the increase in FA-NdfR.


Our results showed that elevated O3 altered the contribution of NdfR to soil N pools. The present study increases our understanding of the dynamics of NdfR and the changes in soil N cycling induced by projected future O3 levels.


Ozone N rhizodeposition Split-root technique 15N labeling N cycling Wheat 







Nitrogen derived from rhizodeposition






Microbial biomass nitrogen


Fixed ammonium nitrogen




Lower ozone concentration-elevated level


Higher ozone concentration-elevated level


Labeling compartment


Transfer compartment


Roots sampled from the transfer compartment


Soil in the transfer compartment

DAF15, DAF30 and DAF45

15, 30 and 45 days after ozone fumigation



This work was supported by the National Natural Science Foundation of China (grant number 41730855; 41703005; 41671290) and the National Key Research and Development Program of China (grant number 2016YFD0800103). We appreciate the anonymous reviewers for insightful comments on an earlier version of our manuscript.

Supplementary material

11104_2018_3592_MOESM1_ESM.docx (22 kb)
Table S1 (DOCX 21 kb)


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Surface-Earth System ScienceTianjin UniversityTianjinChina
  2. 2.Key Laboratory of Pollution Ecology and Environment EngineeringInstitute of Applied Ecology, Chinese Academy of ScienceShenyangChina

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