Plant and Soil

, Volume 424, Issue 1–2, pp 335–349 | Cite as

Bacterial rather than fungal community composition is associated with microbial activities and nutrient-use efficiencies in a paddy soil with short-term organic amendments

  • Junjie Guo
  • Wenbo Liu
  • Chen Zhu
  • Gongwen Luo
  • Yali Kong
  • Ning Ling
  • Min Wang
  • Jingyu Dai
  • Qirong Shen
  • Shiwei Guo
Regular Article


Background and aims

Considering the global demands in sustaining agriculture, use of organic amendments is gradually increasing. An improved understanding of the biological process is essential to evaluate the performance of organic amendments on agro-ecosystem.


Soils subjected to different fertilization regimes were collected from a field experiment. Microbial community compositions are assessed with 16S and ITS rRNA gene sequencing and subsequent bioinformatics analysis. Microbial functions are characterized with the geometric mean of the assayed enzyme activities (GMea) and the microbial carbon-use efficiency:nitrogen-use efficiency ratio (CUE:NUE).


Compared with the chemically fertilized soil, the GMea significantly increased in organically amended soils. In contrast, the CUE:NUE was highest in chemically treated soil. These changes of microbial functional indicators were associated with shifts in the bacterial and not the fungal community composition, despite the fact that both the bacterial and fungal community compositions were significantly affected by the fertilization regimes. The abundances of specific soil bacterial taxa, especially the genera Luteimonas and Gemmatimona, were enriched by organic amendments. Soil organic carbon emerged as the major determinant of the bacterial community composition.


Soil microbial activities and nutrient-use efficiencies were dramatically changed along with the alteration of bacterial community composition. Relatively greater abundance of Luteimonas and Gemmatimona taxa in soils might be useful indicators for soil amelioration. Our research could be helpful to provide better strategies for the maintenance of soil fertility.


Organic amendment Bacterial community Fungal community Microbial activity Microbial nutrient-use efficiencies 



This work was supported by the National Basic Research Program of China (2015CB150500), the National Key Research and Development Program of China (2017YFD0200206) and the Special Fund for Agro-scientific Research in the Public Interest (20150312205).

Supplementary material

11104_2017_3547_MOESM1_ESM.docx (26 kb)
ESM 1 (DOCX 25 kb)


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  • Junjie Guo
    • 1
  • Wenbo Liu
    • 1
  • Chen Zhu
    • 1
  • Gongwen Luo
    • 1
  • Yali Kong
    • 1
  • Ning Ling
    • 1
  • Min Wang
    • 1
  • Jingyu Dai
    • 2
  • Qirong Shen
    • 1
  • Shiwei Guo
    • 1
  1. 1.Jiangsu Provincial Key Laboratory for Solid Organic Waste UtilizationNanjing Agricultural UniversityNanjingChina
  2. 2.College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingChina

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