Microbial community shifts trigger loss of orthophosphate in wetland soils subjected to experimental warming
Microbial-driven biogeochemical cycles of phosphorus (P) in wetlands subjected to global climate warming result in a downstream eutrophication risk. However, how warming influences P associated with microbial shifts in wetland soils is largely unknown.
A custom-built, novel microcosm that simulated climate warming was established under ambient temperature and elevated warming conditions (+ 3 °C). 31P nuclear magnetic resonance (31P–NMR) technology was used to characterize different P forms and high-throughput sequencing of 16S rRNA gene was used to identify microbial community and functional potentials in wetland soils varied with nutrition status.
Soil P forms were dominated by orthophosphate. The dynamic changes of different P forms in response to warming were mainly observed in high nutrition wetlands. The relative abundance of orthophosphate and polyphosphate (inorganic) significantly (p < 0.05) decreased, which was accompanied with increased phosphonate (organic) under warming. Consistently, soil microbial community shifts were also found in high nutrition wetlands, especially in fall with significantly (p < 0.05) increased relative abundance of Alphaproteobacteria and Betaproteobacteria and decreased Clostridia under warming. The microbial functions related to catabolism, the transport, degradation and release of P were enriched under warming. Changed microbial community may have altered the overall functional potentials which were responsible for P dynamics.
Soil microbial community shifts in response to experimental warming were season-based. Microbial changes and P shifts from high nutrition wetlands were more sensitive to warming. The changed microbial community under warming conditions may trigger the loss of orthophosphate through the altered functional potentials. These findings aid to better understand microbial-driven biogeochemical cycles of P in wetland soils under future climate changes.
Keywords31P–NMR 16S rRNA gene Orthophosphate Microbial community Experimental warming Wetland soil
This work was supported by the National Natural Science Foundation of China (41373073, 31500409).
- Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4:9Google Scholar
- Kuffner M, Hai B, Rattei T et al (2012) Effects of season and experimental warming on the bacterial community in a temperate mountain forest soil assessed by 16S rRNA gene pyrosequencing. FEMS Microbiol Ecol 82:551–562. https://doi.org/10.1111/j.1574-6941.2012.01420.x CrossRefPubMedPubMedCentralGoogle Scholar
- Solomon S, et al (ed). 2007. Climate change 2007: the physical science basis. Contribution of Working Group I to the Fourth Assessment Report of the IPCC. Cambridge University Press, CambridgeGoogle Scholar
- Sondergaard M, Jensen JP, Jeppesen E (2003) Role of sediment and internal loading of phosphorus in shallow lakes. Hydrobiologia 506:135–145. https://doi.org/10.1023/B:HYDR.0000008611.12704.dd CrossRefGoogle Scholar
- Wang J, Xue C, Song Y et al (2016b) Wheat and rice growth stages and fertilization regimes alter soil bacterial community structure, but not diversity. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01207
- Wei Y, Wei Z, Cao Z et al (2016) A regulating method for the distribution of phosphorus fractions based on environmental parameters related to the key phosphate-solubilizing bacteria during composting. Bioresour Technol 211:610–617. https://doi.org/10.1016/j.biortech.2016.03.141 CrossRefPubMedGoogle Scholar