Abstract
Background and aims
In the Mediterranean basin, reduction in cloudiness owing to climate change is expected to enhance solar ultraviolet (UV) levels and to decrease rainfall over the coming years, which would be accompanied by more frequent and intense wildfires. The aim of the present study was to investigate the role of solar UV-A and UV-B radiation in C and N pools of a Mediterranean shrubland and whether drier conditions could alter this role before and after a fire.
Methods
Over a three-year field experiment, 18 plots of 9 m2 were subjected to three UV conditions (UV-A + UV-B exclusion, UV-B exclusion or near-ambient UV-A + UV-B exposure) combined with two rainfall regimes (natural or reduced rainfall). Several parameters related to C and N cycles in the soil and in the leaves and litter of two dominant plant species (Arbutus unedo and Phillyrea angustifolia) were measured before and after an experimental fire.
Results
UV-A exposure increased soil moisture throughout the study period, as well as respiration before the fire. The additional presence of UV-B decreased β-glucosidase activity at 5–10 cm depth and soil respiration and pH. UV-B exposure also raised leaf C concentration in P. angustifolia and δ15N values in A. unedo. Reduced rainfall often emphasized the opposite effects of UV-A and UV-B on the studied parameters. After the fire, most of the UV and rainfall effects were lost.
Conclusion
UV-A exposure seems to stimulate soil biological activity and, thus, C and N turn-over, while the effect of UV-B would be the opposite. At least in the short term, the “homogenizing influence” of fire would probably have a stronger effect on the C and N cycles than the expected changes in UV and rainfall levels.
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References
Acácio V, Holmgren M, Rego F et al (2009) Are drought and wildfires turning Mediterranean cork oak forests into persistent shrublands? Agrofor Syst 76:389–400. https://doi.org/10.1007/s10457-008-9165-y
Agati G, Tattini M (2010) Multiple functional roles of flavonoids in photoprotection. New Phytol 186:786–793. https://doi.org/10.1111/j.1469-8137.2010.03269.x
Arévalo JR, de Nascimento L, Fernández-Lugo S et al (2011) Grazing effects on species composition in different vegetation types (La Palma, Canary Islands). Acta Oecol 37:230–238. https://doi.org/10.1016/j.actao.2011.02.006
Austin AT, Ballaré CL (2010) Dual role of lignin in plant litter decomposition in terrestrial ecosystems. Proc Natl Acad Sci 107:4618–4622. https://doi.org/10.1073/pnas.0909396107
Austin AT, Méndez MS, Ballaré CL (2016) Photodegradation alleviates the lignin bottleneck for carbon turnover in terrestrial ecosystems. Proc Natl Acad Sci 113:4392–4397. https://doi.org/10.1073/pnas.1516157113
Baker NR, Allison SD (2015) Ultraviolet photodegradation facilitates microbial litter decomposition in a Mediterranean climate. Ecology 96:1994–2003. https://doi.org/10.1890/14-1482.1
Bassman JH (2004) Ecosystem consequences of enhanced solar ultraviolet radiation: secondary plant metabolites as mediators of multiple trophic interactions in terrestrial plant communities. Photochem Photobiol 79:382–398
Bengtsson J, Janion C, Chown SL, Leinaas HP (2012) Litter decomposition in fynbos vegetation, South Africa. Soil Biol Biochem 47:100–105. https://doi.org/10.1016/j.soilbio.2011.11.023
Brandt LA, King JY, Milchunas DG (2007) Effects of ultraviolet radiation on litter decomposition depend on precipitation and litter chemistry in a shortgrass steppe ecosystem. Glob Chang Biol 13:2193–2205. https://doi.org/10.1111/j.1365-2486.2007.01428.x
Bussotti F (2008) Functional leaf traits, plant communities and acclimation processes in relation to oxidative stress in trees: a critical overview. Glob Chang Biol 14:2727–2739. https://doi.org/10.1111/j.1365-2486.2008.01677.x
Caldwell MM (1971) Solar UV irradiation and the growth and development of higher plants. In: Geise AC (ed) Photophysiology, IV. Academic Press, New York, pp 131–177
Caldwell BA (2005) Enzyme activities as a component of soil biodiversity: a review. Pedobiologia (Jena) 49:637–644. https://doi.org/10.1016/j.pedobi.2005.06.003
Caldwell MM, Bornman JF, Ballaré CL et al (2007) Terrestrial ecosystems, increased solar ultraviolet radiation, and interactions with other climate change factors. Photochem Photobiol Sci 6:252–266. https://doi.org/10.1039/b700019g
Caon L, Vallejo VR, Ritsema CJ, Geissen V (2014) Effects of wildfire on soil nutrients in Mediterranean ecosystems. Earth Sci Rev 139:47–58. https://doi.org/10.1016/j.earscirev.2014.09.001
Castaldi S, Carfora A, Fiorentino A et al (2009) Inhibition of net nitrification activity in a Mediterranean woodland: possible role of chemicals produced by Arbutus unedo. Plant Soil 315:273–283. https://doi.org/10.1007/s11104-008-9750-x
Castells E, Peñuelas J, Valentine DW (2004) Are phenolic compounds released from the Mediterranean shrub Cistus albidus responsible for changes in N cycling in siliceous and calcareous soils? New Phytol 162:187–195. https://doi.org/10.1111/j.1469-8137.2004.01021.x
Certini G (2005) Effects of fire on properties of forest soils: a review. Oecologia 143:1–10. https://doi.org/10.1007/s00442-004-1788-8
Choudhary KK, Pandey D, Agrawal SB (2013) Deterioration of rhizospheric soil health due to elevated ultraviolet-B. Arch Agron Soil Sci 59:1419–1437. https://doi.org/10.1080/03650340.2012.713473
Day TA, Zhang ET, Ruhland CT (2007) Exposure to solar UV-B radiation accelerates mass and lignin loss of Larrea tridentata litter in the Sonoran Desert. Plant Ecol 193:185–194. https://doi.org/10.1007/s11258-006-9257-6
Díaz-Delgado R, Lloret F, Pons X, Terradas J (2002) Satellite evidence of decreasing resilience in Mediterranean plant communities after recurrent wildfires. Ecology 83:2293–2303. https://doi.org/10.1890/0012-9658(2002)083[2293:SEODRI]2.0.CO;2
Dirks I, Navon Y, Kanas D et al (2010) Atmospheric water vapor as driver of litter decomposition in Mediterranean shrubland and grassland during rainless seasons. Glob Chang Biol 16:2799–2812. https://doi.org/10.1111/j.1365-2486.2010.02172.x
Eivazi F, Tabatabai MA (1990) Factors affecting glucosidase and galactosidase activities in soils. Soil Biol Biochem 22:891–897. https://doi.org/10.1016/0038-0717(90)90126-K
El Omari B, Fleck I, Aranda X et al (2003) Total antioxidant activity in Quercus ilex resprouts after fire. Plant Physiol Biochem 41:41–47
Erickson AJ, Ramsewak RS, Smucker AJ, Nair MG (2000) Nitrification inhibitors from the roots of Leucaena leucocephala. J Agric Food Chem 48:6174–6177. https://doi.org/10.1021/jf991382z
Erickson DJ III, Sulzberger B, Zepp RG, Austin AT (2015) Effects of stratospheric ozone depletion, solar UV radiation and climate change on biogeochemical cycling: interactions and feedbacks. Photochem Photobiol Sci 14:127–148. https://doi.org/10.1039/c4pp90036g
Ferréa C, Zenone T, Comolli R, Seufert G (2012) Estimating heterotrophic and autotrophic soil respiration in a semi-natural forest of Lombardy, Italy. Pedobiologia (Jena) 55:285–294. https://doi.org/10.1016/j.pedobi.2012.05.001
Flint SD, Caldwell MM (2003) Field testing of UV biological spectral weighting functions for higher plants. Physiol Plant 117:145–153. https://doi.org/10.1034/j.1399-3054.2003.1170118.x
Foereid B, Bellarby J, Meier-Augenstein W, Kemp H (2010) Does light exposure make plant litter more degradable? Plant Soil 333:275–285. https://doi.org/10.1007/s11104-010-0342-1
Formánek P, Rejšek K, Vranová V (2014) Effect of elevated CO2, O3, and UV radiation on soils. Sci World J 2014:1–8. https://doi.org/10.1155/2014/730149
Forster J (1995) Soil physical analysis. In: Alef K, Nannipieri P (eds) Methods in Soil Microbiology and Biochemistry. Academic Press, London, pp 79–87 105–121
Gallo ME, Sinsabaugh RL, Cabaniss SE (2006) The role of ultraviolet radiation in litter decomposition in arid ecosystems. Appl Soil Ecol 34:82–91. https://doi.org/10.1016/j.apsoil.2005.12.006
Geisseler D, Horwath WR (2009) Relationship between carbon and nitrogen availability and extracellular enzyme activities in soil. Pedobiologia (Jena) 53:87–98. https://doi.org/10.1016/j.pedobi.2009.06.002
Gispert M, Emran M, Pardini G et al (2013) The impact of land management and abandonment on soil enzymatic activity, glomalin content and aggregate stability. Geoderma 202–203:51–61. https://doi.org/10.1016/j.geoderma.2013.03.012
Hart SC, DeLuca TH, Newman GS et al (2005) Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils. For Ecol Manag 220:166–184. https://doi.org/10.1016/j.foreco.2005.08.012
Hayano K, Tubaki K (1985) Origin and properties of β-glucosidase activity of tomato-field soil. Soil Biol Biochem 17:553–557. https://doi.org/10.1016/0038-0717(85)90024-0
Henry HAL, Brizgys K, Field CB (2008) Litter decomposition in a California annual grassland: interactions between photodegradation and litter layer thickness. Ecosystems 11:545–554. https://doi.org/10.1007/s10021-008-9141-4
Hofmann RW, Campbell BD, Bloor SJ et al (2003) Responses to UV-B radiation in Trifolium repens L. – physiological links to plant productivity and water availability. Plant Cell Environ 26:603–612. https://doi.org/10.1046/j.1365-3040.2003.00996.x
Högberg P, Nordgren A, Buchmann N et al (2001) Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature 411:789–792. https://doi.org/10.1038/35081058
Högberg P, Johannisson C, Högberg MN (2014) Is the high 15N natural abundance of trees in N-loaded forests caused by an internal ecosystem N isotope redistribution or a change in the ecosystem N isotope mass balance? Biogeochemistry 117:351–358. https://doi.org/10.1007/s10533-013-9873-x
Hughes KA, Lawley B, Newsham KK (2003) Solar UV-B radiation inhibits the growth of Antarctic terrestrial fungi. Appl Environ Microbiol 69:1488–1491. https://doi.org/10.1128/AEM.69.3.1488–1491.2003
Hurlbert SH (1984) Pseudoreplication and the design of ecological field experiments. Ecol Monogr 54:187–211. https://doi.org/10.2307/1942661
Incerti G, Bonanomi G, Giannino F et al (2011) Litter decomposition in Mediterranean ecosystems: modelling the controlling role of climatic conditions and litter quality. Appl Soil Ecol 49:148–157. https://doi.org/10.1016/j.apsoil.2011.06.004
IPCC (2013) Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, and New York
Julkunen-Tiitto R, Häggman H, Aphalo PJ et al (2005) Growth and defense in deciduous trees and shrubs under UV-B. Environ Pollut 137:404–414. https://doi.org/10.1016/j.envpol.2005.01.050
Knicker H (2007) How does fire affect the nature and stability of soil organic nitrogen and carbon? A review. Biogeochemistry 85:91–118. https://doi.org/10.1007/s10533-007-9104-4
Li F-R, Peng S-L, Chen B-M, Hou Y-P (2010) A meta-analysis of the responses of woody and herbaceous plants to elevated ultraviolet-B radiation. Acta Oecol 36:1–9. https://doi.org/10.1016/j.actao.2009.09.002
Llorens L, Peñuelas J, Estiarte M (2003) Ecophysiological responses of two Mediterranean shrubs, Erica multiflora and Globularia alypum, to experimentally drier and warmer conditions. Physiol Plant 119:231–243. https://doi.org/10.1034/j.1399-3054.2003.00174.x
Lloret F, Calvo E, Pons X, Díaz-Delgado R (2002) Wildfires and landscape patterns in the eastern Iberian peninsula. Landsc Ecol 17:745–759. https://doi.org/10.1023/A:1022966930861
López-Poma R, Bautista S (2014) Plant regeneration functional groups modulate the response to fire of soil enzyme activities in a Mediterranean shrubland. Soil Biol Biochem 79:5–13. https://doi.org/10.1016/j.soilbio.2014.08.016
Lu Y, Duan B, Zhang X et al (2009) Intraspecific variation in drought response of Populus cathayana grown under ambient and enhanced UV-B radiation. Ann For Sci 66:613–624. https://doi.org/10.1051/forest/2009049
Masciandaro G, Ceccanti B, García C (1994) Anaerobic digestion of straw and piggery wastewaters II. Optimization of the process. Agrochimica 38:195–203
Matteucci M, Gruening C, Ballarin IG et al (2015) Components, drivers and temporal dynamics of ecosystem respiration in a Mediterranean pine forest. Soil Biol Biochem 88:224–235. https://doi.org/10.1016/j.soilbio.2015.05.017
Nannipieri P, Kandeler E, Ruggiero P (2002) Enzyme activities and microbiological and biochemical processes in soil. In: Burns RG, Dick RP (eds) Enzymes in the environment: activity, ecology and applications. Marcel Dekker, New York, pp 1–33
Nenadis N, Llorens L, Koufogianni A et al (2015) Interactive effects of UV radiation and reduced precipitation on the seasonal leaf phenolic content/composition and the antioxidant activity of naturally growing Arbutus unedo plants. J Photochem Photobiol B Biol 153:435–444. https://doi.org/10.1016/j.jphotobiol.2015.10.016
Ogaya R, Peñuelas J (2008) Changes in leaf δ13C and δ15N for three Mediterranean tree species in relation to soil water availability. Acta Oecol 34:331–338. https://doi.org/10.1016/j.actao.2008.06.005
Pancotto VA, Sala OE, Robson TM et al (2005) Direct and indirect effects of solar ultraviolet-B radiation on long-term decomposition. Glob Chang Biol 11:1982–1989. https://doi.org/10.1111/j.1365-2486.2005.01027.x
Pardo LH, Hemond HF, Montoya JP, Pett-Ridge J (2007) Natural abundance 15N in soil and litter across a nitrate-output gradient in New Hampshire. For Ecol Manag 251:217–230. https://doi.org/10.1016/j.foreco.2007.06.047
Paula S, Pausas JG (2006) Leaf traits and resprouting ability in the Mediterranean basin. Funct Ecol 20:941–947. https://doi.org/10.1111/j.1365-2435.2006.01185.x
Pausas JG, Bradstock RA, Keith DA et al (2004) Plant functional traits in relation to fire in crown-fire ecosystems. Ecology 85:1085–1100
Raich JW, Tufekcioglu A (2000) Vegetation and soil respiration: correlations and controls. Biogeochemistry 48:71–90
Ren J, Dai W, Xuan Z et al (2007) The effect of drought and enhanced UV-B radiation on the growth and physiological traits of two contrasting poplar species. For Ecol Manag 239:112–119. https://doi.org/10.1016/j.foreco.2006.11.014
Rey A, Pegoraro E, Tedeschi V et al (2002) Annual variation in soil respiration and its components in a coppice oak forest in Central Italy. Glob Chang Biol 8:851–866. https://doi.org/10.1046/j.1365-2486.2002.00521.x
Riera P, Peñuelas J, Farreras V, Estiarte M (2007) Valuation of climate-change effects on Mediterranean shrublands. Ecol Appl 17:91–100. https://doi.org/10.1890/1051-0761(2007)017[0091:VOCEOM]2.0.CO;2
Rinnan R, Gehrke C, Michelsen A (2006) Two mire species respond differently to enhanced ultraviolet-B radiation: effects on biomass allocation and root exudation. New Phytol 169:809–818. https://doi.org/10.1111/j.1469-8137.2005.01577.x
Rinnan R, Nerg AM, Ahtoniemi P et al (2008) Plant-mediated effects of elevated ultraviolet-B radiation on peat microbial communities of a subarctic mire. Glob Chang Biol 14:925–937. https://doi.org/10.1111/j.1365-2486.2008.01544.x
Ruiz-Navarro A, Barberá GG, Albaladejo J, Querejeta JI (2016) Plant δ15N reflects the high landscape-scale heterogeneity of soil fertility and vegetation productivity in a Mediterranean semiarid ecosystem. New Phytol 212:1030–1043. https://doi.org/10.1111/nph.14091
Sanchez-Lorenzo A, Enriquez-Alonso A, Calbó J et al (2017) Fewer clouds in the Mediterranean: consistency of observations and climate simulations. Sci Rep 7:41475. https://doi.org/10.1038/srep41475
Sardans J, Peñuelas J (2013) Plant-soil interactions in Mediterranean forest and shrublands: impacts of climatic change. Plant Soil 365:1–33. https://doi.org/10.1007/s11104-013-1591-6
Sardans J, Peñuelas J, Estiarte M (2008a) Changes in soil enzymes related to C and N cycle and in soil C and N content under prolonged warming and drought in a Mediterranean shrubland. Appl Soil Ecol 39:223–235. https://doi.org/10.1016/j.apsoil.2007.12.011
Sardans J, Peñuelas J, Estiarte M, Prieto P (2008b) Warming and drought alter C and N concentration, allocation and accumulation in a Mediterranean shrubland. Glob Chang Biol 14:2304–2316. https://doi.org/10.1111/j.1365-2486.2008.01656.x
Sardans J, Rivas-Ubach A, Peñuelas J (2012) The C:N:P stoichiometry of organisms and ecosystems in a changing world: a review and perspectives. Perspect Plant Ecol Evol Syst 14:33–47. https://doi.org/10.1016/j.ppees.2011.08.002
Savé R, Alegre L, Pery M, Terradas J (1993) Ecophysiology of after-fire resprouts of Arbutus unedo L. Orsis 8:107–119
Searles PS, Flint SD, Caldwell MM (2001) A meta-analysis of plant field studies simulating stratospheric ozone depletion. Oecologia 127:1–10. https://doi.org/10.1007/s004420000592
Sherman C, Sternberg M, Steinberger Y (2012) Effects of climate change on soil respiration and carbon processing in Mediterranean and semi-arid regions: an experimental approach. Eur J Soil Biol 52:48–58. https://doi.org/10.1016/j.ejsobi.2012.06.001
Soil Survey Staff (2010) Keys to soil taxonomy. Eleventh, USDA-NRCS, Washington, DC
Szpak P (2014) Complexities of nitrogen isotope biogeochemistry in plant-soil systems: implications for the study of ancient agricultural and animal management practices. Front Plant Sci 5:1–19. https://doi.org/10.3389/fpls.2014.00288
Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307. https://doi.org/10.1016/0038-0717(69)90012-1
Talmon Y, Sternberg M, Grünzweig JM (2011) Impact of rainfall manipulations and biotic controls on soil respiration in Mediterranean and desert ecosystems along an aridity gradient. Glob Chang Biol 17:1108–1118. https://doi.org/10.1111/j.1365-2486.2010.02285.x
Tárrega R, Luis-Calabuig E, Valbuena L (2001) Eleven years of recovery dynamic after experimental burning and cutting in two Cistus communities. Acta Oecol 22:277–283. https://doi.org/10.1016/S1146-609X(01)01125-0
Verdaguer D, Ojeda F (2002) Root starch storage and allocation patterns in seeder and resprouter seedlings of two cape Erica (Ericaceae) species. Am J Bot 89:1189–1196. https://doi.org/10.3732/ajb.89.8.1189
Yue M, Li Y, Wang X (1998) Effects of enhanced ultraviolet-B radiation on plant nutrients and decomposition of spring wheat under field conditions. Environ Exp Bot 40:187–196. https://doi.org/10.1016/S0098-8472(98)00036-7
Zepp RG, Callaghan TV, Erickson DJ (1998) Effects of enhanced solar ultraviolet radiation on biogeochemical cycles. J Photochem Photobiol B Biol 46:69–82. https://doi.org/10.1016/S1011-1344(98)00186-9
Zepp RG, Erickson DJ III, Paul ND, Sulzberger B (2007) Interactive effects of solar UV radiation and climate change on biogeochemical cycling. Photochem Photobiol Sci 6:286–300. https://doi.org/10.1039/b700021a
Acknowledgements
This research was supported by the Spanish Government (CGL2010-22283 and CGL2014-55976-R) and the University of Girona (ASING2011/3 and MPCUdG2016). We are grateful to the Gavarres Consortium for allowing us to perform the experiment in Can Vilallonga. We also thank Jordi Compte, Meritxell Bernal and Miquel Jover for their help with the field experiment, and Dr. Alan Jones for his comments to improve the manuscript.
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Díaz-Guerra, L., Verdaguer, D., Gispert, M. et al. Effects of UV radiation and rainfall reduction on leaf and soil parameters related to C and N cycles of a Mediterranean shrubland before and after a controlled fire. Plant Soil 424, 503–524 (2018). https://doi.org/10.1007/s11104-017-3485-5
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DOI: https://doi.org/10.1007/s11104-017-3485-5