Involvement of reactive oxygen species and Ca2+ in the differential responses to low-boron in rapeseed genotypes
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Background and aims
Boron (B) deficiency significantly inhibits plant growth and development. Oilseed rape (Brassica napus L.) is highly susceptible to B deficiency. Reactive oxygen species (ROS) and Ca2+ play pivotal roles in plant responses to environmental stresses. We aim to identify the differential Ca2+ fluxes and ROS bursts of a B-efficient genotype ‘QY10’ and a B-inefficient genotype ‘W10’ to B deficiency, and establish a signalling pathway involving Ca2+ and ROS implicated in the low-B-induced cell death.
Under both plant and suspension cell systems, the ROS production was investigated histochemically, cytochemically and biochemically; K+ and Ca2+ effluxes were assayed using the Non-invasive Micro-test Technology (NMT); the expression of ROS-producing genes and the activity assays of antioxidant enzymes were tested, and the ROS scavengers and Ca2+ channel inhibitors were used to characterize the roles of ROS and Ca2+ in response to low-B, respectively.
The cell death was mainly responsible for rapeseed growth inhibition under B deficiency. Low-B induced O2 − accumulation, whose distribution was similar to the cell death regions in the plant roots. The increase in O2 − production was much stronger in ‘W10’ than in ‘QY10’. The change trend of H2O2 was similar to that of O2 −, whereas less significant. The enhancement of lipid peroxidation, ion leakage and K+ efflux indicated that low-B caused cell death through the induction of oxidative damages, particularly in ‘W10’. Pretreatment with O2 − scavenger increased the cell viabilities. Low-B induced Ca2+ influx, which worked upstream of ROS. It was not the antioxidant enzymes but the ROS-generating enzymes that determined the differential oxidative damages in rapeseed genotypes.
Low-B induced Ca2+ influx, which then stimulated the ROS burst and eventually caused cell death. The present study enriches our understanding of the involvement of ROS and Ca2+ in the differential responses to B deficiency in rapeseed genotypes.
KeywordsBoron deficiency Brassica napus Ca2+ Genotypes Reactive oxygen species
Non-invasive Micro-test Technology
Programmed cell death
Quantitative real-time PCR
Respiratory Burst Oxidase Homolog
Reactive oxygen species
This work was financially supported by the National Natural Science Foundation of China (Grant NO. 31372129, 31572185) and the National Key Research and Development Program of China (Grant NO. 2016YFD0100700).
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