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Plant and Soil

, Volume 419, Issue 1–2, pp 141–152 | Cite as

Hydrogen sulfide mediates ion fluxes inducing stomatal closure in response to drought stress in Arabidopsis thaliana

  • Zhuping Jin
  • Zhiqing Wang
  • Qingxia Ma
  • Limin Sun
  • Liping Zhang
  • Zhiqiang Liu
  • Danmei Liu
  • Xuefeng Hao
  • Yanxi Pei
Regular Article

Abstract

Background and aims

In many plant species, hydrogen sulfide (H2S) triggers stomatal closure, which is produced mainly by two classes of enzymes, cysteine desulfhydrases (CDes) and O-acetyl-L-serine (thiol) lyases (OASTLs). Stomatal movement is accompanied by several ion fluxes across the plasma membranes of guard cells.

Methods

In this paper, we detected the fluxes of H+, Ca2+, K+ and Cl in guard cells of wild-type Arabidopsis thaliana and the mutants associated with H2S production (lcd, OE-LCD, des, OE-DES, oastl-a1, oastl-a2, oastl-b and oastl-c), using a non-invasive micro-test technique.

Results

The results showed that endogenous H2S induced a transmembrane K+ efflux, and Ca2+ and Cl influxes, while not affecting the flow of H+. Furthermore, the K+ channel was the main osmolyte responder during the regulation of stomatal movement by H2S in response to drought stress. Finally, the two classes of enzymes produced H2S, CDes and OASTLs, played different roles in regulating stomatal movements.

Conclusions

Thus, H2S mediates ion fluxes inducing stomatal closure in response to drought stress in Arabidopsis thaliana.

Keywords

Hydrogen sulfide Ion flux Stoma Guard cell Drought stress Arabidopsis thaliana 

Abbreviations

H2S

Hydrogen sulfide

LCD

L-cysteine desulfhydrase

DES

desulfhydrase

CDes

cysteine desulfhydrases

OASTLs

O-acetyl-L-serine (thiol) lyases

Cys

cysteine

[Ca2+]cyt

cytosolic-free Ca2+

NMT

non-invasive micro-test technique

OE

overexpression mutants

ABA

abscisic acid

H2O2

hydrogen peroxide

NO

nitric oxide

Notes

Acknowledgements

This work was funded by a grant from the National Natural Science Foundation of China (grant nos. 31672140 and 31400237 to Jin Z., 31671605 to Pei Y.) and University Science and technology innovation project (grant nos. 2016110 to Jin Z.). The authors have no conflict of interest to declare.

Supplementary material

11104_2017_3335_MOESM1_ESM.pdf (87 kb)
Table S1 Mutants information. (PDF 86 kb)
11104_2017_3335_MOESM2_ESM.jpg (1.4 mb)
Supplementary Figure S1 Water loss of leaves in different lines. A, Rate of water loss of leaves from col., lcd, OE-LCD, des, OE-DES, oastl-a1, oastl-a2, oastl-b and oastl-c maintained at 105 °C within the measuring period are shown. B, The relative water content of leaves in different lines. Bars represent the standard error of three independent repeats. Lowercase letters indicate statistical differences (P < 0.05) as determined by Tukey’s multiple range test. (JPEG 1453 kb)
11104_2017_3335_MOESM3_ESM.jpg (3 mb)
Supplementary Figure S2 Net fluxes of H+, Ca2+, Cl in the lower epidermal tissues isolated from leaves of different lines in normal conditions. A continuous flux recording of 10 min was conducted for each guard cell in a corresponding measuring solution (pH 6.0) for A) H+; B) Ca2+; and C) Cl. The data were collected every 6 s during the 10-min measuring period. (JPEG 3115 kb)

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.School of Life ScienceShanxi UniversityTaiyuanChina

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