Hydrogen sulfide mediates ion fluxes inducing stomatal closure in response to drought stress in Arabidopsis thaliana
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Background and aims
In many plant species, hydrogen sulfide (H2S) triggers stomatal closure, which is produced mainly by two classes of enzymes, cysteine desulfhydrases (CDes) and O-acetyl-L-serine (thiol) lyases (OASTLs). Stomatal movement is accompanied by several ion fluxes across the plasma membranes of guard cells.
In this paper, we detected the fluxes of H+, Ca2+, K+ and Cl− in guard cells of wild-type Arabidopsis thaliana and the mutants associated with H2S production (lcd, OE-LCD, des, OE-DES, oastl-a1, oastl-a2, oastl-b and oastl-c), using a non-invasive micro-test technique.
The results showed that endogenous H2S induced a transmembrane K+ efflux, and Ca2+ and Cl− influxes, while not affecting the flow of H+. Furthermore, the K+ channel was the main osmolyte responder during the regulation of stomatal movement by H2S in response to drought stress. Finally, the two classes of enzymes produced H2S, CDes and OASTLs, played different roles in regulating stomatal movements.
Thus, H2S mediates ion fluxes inducing stomatal closure in response to drought stress in Arabidopsis thaliana.
KeywordsHydrogen sulfide Ion flux Stoma Guard cell Drought stress Arabidopsis thaliana
O-acetyl-L-serine (thiol) lyases
non-invasive micro-test technique
This work was funded by a grant from the National Natural Science Foundation of China (grant nos. 31672140 and 31400237 to Jin Z., 31671605 to Pei Y.) and University Science and technology innovation project (grant nos. 2016110 to Jin Z.). The authors have no conflict of interest to declare.
- Jin Z, Pei Y (2015) Physiological implications of hydrogen sulfide in plants: pleasant exploration behind its unpleasant odour. Oxidative Med Cell Longev 2015:397502Google Scholar
- Kwak JM, Murata Y, Baizabal-Aguirre VM, Merrill J, Wang M, Kemper A, Hawke SD, Tallman G, Schroeder JI (2001) Dominant negative guard cell K+ channel mutants reduce inward-rectifying K+ currents and light-induced stomatal opening in Arabidopsis. Plant Physiol 127:473–485CrossRefPubMedPubMedCentralGoogle Scholar
- Sun J, Chen S, Dai S, Wang R, Li N, Shen X, Zhou X, Lu C, Zheng X, Hu Z, Zhang Z, Song J, Xu Y (2009) NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Plant Physiol 149:1141–1153Google Scholar
- Yang GD (2014) Protein S-sulfhydration as a major sources of H2S bioactivity. Receptors Clin Investig. 1 (6): 10-14800/rci.337Google Scholar