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Redefining the dose of the entomopathogenic fungus Metarhizium brunneum (Ascomycota, Hypocreales) to increase Fe bioavailability and promote plant growth in calcareous and sandy soils

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Abstract

Background and aims

Entomopathogenic mitosporic ascomycetes Beauveria, Metarhizium and Isaria sp. are commonly used for pest control but can also serve other, lesser known functions such as increasing nutrient bioavailability or promote plant growth. The objective of this work was to identify the doses of entomopathogenic fungi (EF) to be applied to soil in order to modify iron (Fe) uptake by plants and promote their growth.

Methods

We used an in vitro assay to assess the ability of Beauveria bassiana, Metarhizium brunneum and Isaria farinosa to mobilize Fe from nine Fe oxides differing in composition, particle size and crystallinity, including ferrihydrite, hematite, goethite and magnetite. We also conducted an in vivo assay by applying five different doses (viz., 0, 5 × 102, 5 × 104, 5 × 106 and 5 × 108 conidia ml−1) of a conidial suspension of M. brunneum to the surface of a calcareous soil, which induced Fe chlorosis and a non–calcareous soil which did not induce chlorosis to explore the ability of the fungus on improving Fe nutrition and plant growth of sorghum and sunflower plants.

Results

In the in vitro assay, all three EF increased Fe availability differently depending on particle size and crystallinity, and I. farinosa and B. bassiana increased the pH of the culture medium, whereas M. brunneum did not produce a great effect. In the in vivo assay, the highest dose (5 × 108 conidia ml−1) of M. brunneum alleviated Fe chlorosis symptoms of sorghum plants grown in the calcareous soil, and the two highest doses (5 × 106 and 5 × 108 conidia ml−1) increased plant height and inflorescence production of sunflower grown in both soils.

Conclusions

The observed benefits of EF on plant growth and nutrition provide support for more sustainable and cost–effective use of these biocontrol agents.

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Acknowledgements

This work was funded by the Innovation, Science and Enterprise Council of the Andalusian Regional Government (Project AGR– 7681). We thank the Servicio Central de Apoyo a la Investigación (SCAI) of the University of Córdoba for technical support in using its X–ray diffractometer and transmission electron microscope.

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Authors and Affiliations

  1. Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, ETSIAM, 14071, Córdoba, Spain

    Silvia Raya–Díaz, Enrique Quesada–Moraga & Antonio Rafael Sánchez–Rodríguez

  2. Departamento de Agronomía, Universidad de Córdoba, ETSIAM, 14071, Córdoba, Spain

    Vidal Barrón, María Carmen del Campillo & Antonio Rafael Sánchez–Rodríguez

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  1. Silvia Raya–Díaz
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  2. Enrique Quesada–Moraga
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Correspondence to Antonio Rafael Sánchez–Rodríguez.

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Raya–Díaz, S., Quesada–Moraga, E., Barrón, V. et al. Redefining the dose of the entomopathogenic fungus Metarhizium brunneum (Ascomycota, Hypocreales) to increase Fe bioavailability and promote plant growth in calcareous and sandy soils. Plant Soil 418, 387–404 (2017). https://doi.org/10.1007/s11104-017-3303-0

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