Trichoderma-enriched organic fertilizer can mitigate microbiome degeneration of monocropped soil to maintain better plant growth
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Investigating shifts in soil microbiomes driven by different fertilization regimes would be helpful for lessening the negative effect of monoculture in agricultural systems.
In the present work, we employed MiSeq sequencing to evaluate the response of local microbial communities to three different fertilization regimes, i.e., heavy chemical fertilizer application (CF) and reduced chemical fertilizer applications supplemented with organic (OF) or Trichoderma-enriched organic fertilizer (BF), in a continuous five-season pot experiment on tomato.
The CF-treated soil resulted in a bacterial community with the lowest diversity, while the BF-treated soil had the highest diversity level. The OF-treated soil had the lowest diversity in the fungal community, while the CF- and BF-treated soils had higher diversity. Moreover, better plant growth and soil fertility status were obtained in the BF treatment followed by the OF and CF treatments.
Compared to the CF and OF regimes, reduced chemical fertilizer plus Trichoderma-enriched organic fertilizer (BF) is the most suitable regime to control microbiome degeneration of monocropped soil and to thus maintain tomato plant growth and health.
KeywordsTomato monocropping Soil microbiome MiSeq sequencing Soil nutrient characteristics
This research was financially supported by 973 program (2015CB150500) and National Key Technology R&D Program of the Ministry of Science and Technology of China (2013BAD20B05 and L0201400202) and Jiangsu Science and Technology Department (BK20150059).
- Besemer K, Peter H, Logue JB, et al (2012) Unraveling assembly of stream biofilm communities. ISME J 6:1459–1468. doi: 10.1038/ismej.2011.205
- Cai F, Yu G, Wang P, et al (2013) Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum. Plant Physiol Biochem 73:106–113. doi: 10.1016/j.plaphy.2013.08.011
- Leaw SN, Chang HC, Sun HF, et al (2006) Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J Clin Microbiol 44:693–699. doi: 10.1128/JCM.44.3.693-699.2006
- Lichtfouse E (ed) (2011) Genetics, biofuels and local farming systems. Springer Netherlands, DordrechtGoogle Scholar
- Somai BM, Dean RA, Farnham MW, et al (2002) Internal transcribed spacer regions 1 and 2 and random amplified polymorphic DNA analysis of Didymella bryoniae and related Phoma species isolated from cucurbits. Phytopathology 92:997–1004. doi: 10.1094/PHYTO.2002.92.9.997
- ter Braak CJF, Šmilauer P (2002) CANOCO reference manual and CanoDraw for windows user’s guide: Software for canonical community ordination (version 4.5). Biometris, Ithaca NYGoogle Scholar
- Trillas MI, Segarra G (2009) Interactions between nonpathogenic fungi and plants. In: van Loon LC (ed) Advances in botanical research. Elsevier, Amsterdam, pp 321–359. doi: 10.1016/S0065-2296(09)51008-7Google Scholar
- Xun W, Huang T, Zhao J et al (2015) Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities. Soil Biol Biochem 90:10–18. doi: 10.1016/j.soilbio.2015.07.018 CrossRefGoogle Scholar