Plant and Soil

, Volume 415, Issue 1–2, pp 269–281

Application of biochar reduces Ralstonia solanacearum infection via effects on pathogen chemotaxis, swarming motility, and root exudate adsorption

  • Yian Gu
  • Yugang Hou
  • Dapeng Huang
  • Zhexia Hao
  • Xiaofang Wang
  • Zhong Wei
  • Alexandre Jousset
  • Shiyong Tan
  • Dabing Xu
  • Qirong Shen
  • Yangchun Xu
  • Ville-Petri Friman
Regular Article

Abstract

Aims

We evaluated the efficacy of biochar application for suppressing bacterial wilt of tomato and identified the potential underlying mechanisms involved in the disease control.

Methods

We measured the impact of two different sized biochar (53–120 μm and 380–830 μm) on bacterial wilt incidence in a greenhouse experiment. The efficiency of different sized biochar for the adsorption of tomato root exudates and the pathogen was further examined in vitro. We also quantified the effects of biochar and tomato root exudates on two pathogen virulence factors, chemotaxis, swarming motility and examined the effect of biochar on pathogen root colonization.

Results

Fine biochar application (3%; w:w) significantly decreased the bacterial wilt incidence by 19.9%. Biochar with different particle size had similar adsorption capacity for root exudates, while fine biochar was efficient (91%) in pathogen adsorption. Root exudates and fine biochar increased the chemotaxis ability of pathogen, while fine biochar reduced pathogen swarming motility and rhizosphere colonization.

Conclusions

Application of fine biochar can significantly decreased bacterial wilt incidence. This was mechanistically explained by biochar ability to 1) adsorb pathogen directly and indirectly via adsorption of root exudates (based on pathogen chemotaxis) and to 2) directly suppress pathogen swarming motility and subsequent root colonization.

Keywords

Adsorption Bacterial wilt Biochar Disease control Root exudate 

Supplementary material

11104_2016_3159_Fig6_ESM.gif (40 kb)
Fig. S1

Dose-response for the adsorption of R. solanacearum QL-Rs1115 by biochar with different particle size. Error bars indicate the standard error of the mean. Different letters indicate significant differences between different treatments. (GIF 40 kb)

11104_2016_3159_MOESM1_ESM.tif (231 kb)
High resolution image (TIFF 230 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Yian Gu
    • 1
  • Yugang Hou
    • 1
  • Dapeng Huang
    • 1
  • Zhexia Hao
    • 1
  • Xiaofang Wang
    • 1
  • Zhong Wei
    • 1
  • Alexandre Jousset
    • 1
    • 2
  • Shiyong Tan
    • 3
  • Dabing Xu
    • 4
  • Qirong Shen
    • 1
  • Yangchun Xu
    • 1
  • Ville-Petri Friman
    • 5
  1. 1.Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based FertilizersNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Institute for Environmental Biology, Ecology & BiodiversityUtrecht UniversityUtrechtThe Netherlands
  3. 3.Key Laboratory of Plant Nutrition and Biological Fertilizer, Ministry of AgricultureHunan Taigu Biotechnology Co., LtdChangshaPeople’s Republic of China
  4. 4.Plant Protection and Soil Fertilizer InstituteHubei Academy of Agricultural SciencesWuhanPeople’s Republic of China
  5. 5.Department of BiologyUniversity of YorkYorkUK

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