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Fungal diversity associated with pulses and its influence on the subsequent wheat crop in the Canadian prairies

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Abstract

Background and aims

Variations in root-associated fungal communities contribute to the so-called ‘crop rotation benefit’ on soil productivity. We assessed the effects of chickpea, lentil, and pea in wheat-based rotations, as compared to wheat monoculture, on the structure of root-associated fungal communities, and described the legacy of pulses on a following wheat crop.

Methods

The internal transcribed spacer (ITS) and 18S rRNA gene markers, and 454 amplicon pyrosequencing were used to describe the fungal communities of crop roots and rhizosphere soil in a field experiment and agronomic data were collected.

Results

Pulses influenced only the structure of the non-mycorrhizal fungal community of roots. Fusarium tricinctum, Clonostachys rosea, Fusarium redolens, and Cryptococcus sp. were specific to certain crops. Despite the absence of selective effects of pulses on their associated arbuscular mycorrhizal (AM) fungal community, pea had a legacy effect on the structure of the AM fungal community associated with the roots of the following wheat crop, in one of the two year/sites examined. Species of Mortierella, Cryptococcus, and Paraglomus in wheat rhizosphere soil may benefit yield, whereas species of Fusarium, Davidiella, Lachnum, Sistotrema and Podospora may reduce yield.

Conclusion

The effect of pulse crops on root fungal communities varied with rotation crop species. Pulses had various effects on the physiology of the following wheat crop, including increased productivity.

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Acknowledgments

The authors would like to thank Keith Hanson, Elijah Atuku, Cal McDonald, and Lee Poppy for their technical assistance. This work was supported by Saskatchewan Pulse Growers and the Growing Forward Program of the Government of Canada.

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Authors and Affiliations

  1. Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, 1 Airport Road, Swift Current, SK, S9H 3X2, Canada

    A. Navarro Borrell, Y. Gan, L. D. Bainard & C. Hamel

  2. University of Saskatchewan, 204 Peter MacKinnon Building, 107 Administration Place, Saskatoon, SK, S7N 5A2, Canada

    A. Navarro Borrell, J. J. Germida & C. Hamel

  3. Centre for Applied Arts and Sciences, Lethbridge College, 3000 College Drive South, Lethbridge, AB, T1K 1L6, Canada

    A. Navarro Borrell

  4. Quebec Research and Development Centre, Agriculture and Agri-Food Canada, 2560 Hochelaga Boulevard, QC, Québec, G1V 2J3, Canada

    Y. Shi & C. Hamel

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  1. A. Navarro Borrell
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  2. Y. Shi
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  3. Y. Gan
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  4. L. D. Bainard
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Correspondence to C. Hamel.

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Borrell, A.N., Shi, Y., Gan, Y. et al. Fungal diversity associated with pulses and its influence on the subsequent wheat crop in the Canadian prairies. Plant Soil 414, 13–31 (2017). https://doi.org/10.1007/s11104-016-3075-y

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