Plant and Soil

, Volume 411, Issue 1–2, pp 81–99 | Cite as

QTL mapping for grain zinc and iron concentrations and zinc efficiency in a tetraploid and hexaploid wheat mapping populations

  • Govindan Velu
  • Yusuf Tutus
  • Hugo F. Gomez-Becerra
  • Yuanfeng Hao
  • Lütfü Demir
  • Rukiye Kara
  • Leonardo A. Crespo-Herrera
  • Sinasi Orhan
  • Atilla Yazici
  • Ravi P. Singh
  • Ismail Cakmak
Regular Article

Abstract

Background and aims

Zinc (Zn) and iron (Fe) deficiencies are the most important forms of malnutrition globally, and caused mainly by low dietary intake. Wheat, a major staple food crop, is inherently low in these micronutrients. Identifying new QTLs for high grain Zn (GZn) and Fe (GFe) will contribute to improved micronutrient density in wheat grain.

Methods

Using two recently developed RIL mapping populations derived from a wild progenitor of a tetraploid population “Saricanak98 × MM5/4” and an hexaploid population “Adana99 × 70,711”, multi-locational field experiments were conducted over 2 years to identify genomic regions associated with high grain Zn (GZn) and grain Fe (GFe) concentrations. Additionally, a greenhouse experiment was conducted by growing the “Saricanak98 × MM5/4” population in a Zn-deficient calcareous soil to determine the markers involved in Zn efficiency (ZnEff) of the genotypes (expressed as the ratio of shoot dry weight under Zn deficiency to Zn fertilization) and its relation to GZn. The populations were genotyped by using DArT markers.

Results

Quantitative trait loci (QTL) for high GFe and GZn concentrations in wheat grains were mapped in the both RIL mapping populations. Two major QTLs for increasing GZn were stably detected on chromosomes 1B and 6B of the tetra- and hexaploid mapping populations, and a GZn QTL on chromosome 2B co-located with grain GFe, suggesting simultaneous improvement of GFe and GZn is possible. In the greenhouse experiment, the RILs exhibited substantial genotypic variation for Zn efficiency ratio, ranging from 31 % to 90 %. Two QTL for Zn efficiency were identified on chromosomes 6A and 6B. There was no association between Zn efficiency and grain Zn concentration among the genotypes. The results clearly show that Zn efficiency and Zn accumulation in grain are governed by different genetic mechanisms.

Conclusion

Identification of some consistent genomic regions such as 1B and 6B across two different mapping populations suggest these genomic regions might be the useful regions for further marker development and use in biofortification breeding programs.

Keywords

Biofortification Iron Zinc Zinc deficiency Mapping population QTL Wheat 

Abbreviations

GZn

Grain zinc

GFe

Grain iron

ZnEff

Zn efficiency

QTL

Quantitative trait loci

PVE

phenotypic variation explained

Notes

Acknowledgments

The authors acknowledge financial support from the HarvestPlus Challenge Program to Sabanci University and CIMMYT, and thanks to the Directors of the Maize Research Institute-Sakarya (Mr. Yavuz Agi) and East Mediterranean Transitional Zone Agricultural Research of Institute-Kahramanmaras (Mr. Hasan Gezginc) for their great support for the establishment of the field experiments in Turkey. Authors are also grateful to Prof Dr. Hakan Ozkan (Cukurova University) for providing seed material.

Supplementary material

11104_2016_3025_MOESM1_ESM.docx (1.3 mb)
ESM 1(DOCX 1344 kb)

References

  1. Blair MW, Sandoval TA, Caldas GV, Beebe SE, Paez MI (2009) Quantitative trait locus analysis of seed phosphorus and seed phytate content in a recombinant inbred line population of common bean. Crop Sci 49:237–246CrossRefGoogle Scholar
  2. Bonnett D, Rebetzke GJ, Spielmeyer W (2005) Strategies for efficient implementation of molecular markers in wheat breeding. Mol Breed 15:75–85CrossRefGoogle Scholar
  3. Bouis HE, Welch RM (2010) Biofortification- a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci 50:20–32CrossRefGoogle Scholar
  4. Briat JF, Dubas C, Gaymard F (2015) Iron nutrition, biomass production, and plant product quality. Trends Plant Sci 20:33–40CrossRefPubMedGoogle Scholar
  5. Broman KW, Wu H, Sen Ś, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889–890CrossRefPubMedGoogle Scholar
  6. Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17CrossRefGoogle Scholar
  7. Cakmak I, Ekiz H, Yılmaz A, Torun B, Köleli N, Gültekin I, Alkan A, Eker S (1997) Differential response of rye, triticale, bread and durum wheats to zinc deficiency in calcareous soils. Plant Soil 188:1–10CrossRefGoogle Scholar
  8. Cakmak I, Torun B, Erenoglu B, Oztürk L, Marschner H, Kalaycı M, Ekiz H, Yılmaz A (1998) Morphological and physiological differences in cereals in response to zinc deficiency. Euphytica 100:349–357CrossRefGoogle Scholar
  9. Cakmak I, Tolay I, Ozdemir A, Ozkan H, Kling CI (1999) Differences in zinc efficiency among and within diploid, tetraploid and hexaploid wheats. Ann Bot 84:163–171CrossRefGoogle Scholar
  10. Cakmak I, Torun A, Millet E, Feldman M, Fahima T, Korol A, Nevo E, Braun HJ, Ozkan H (2004) Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Sci Plant Nutr 50:1047–1054CrossRefGoogle Scholar
  11. Cakmak I, Pfeiffer WH, McClafferty B (2010) Biofortification of durum wheat with zinc and iron. Cereal Chem 87:10–20CrossRefGoogle Scholar
  12. Chatzav M, Peleg Z, Ozturk L, Yazici A, Fahima T, Cakmak I, Saranga Y (2010) Genetic diversity for grain nutrients in wild emmer wheat: potential for wheat improvement. Ann Bot 105:1211–1220CrossRefPubMedPubMedCentralGoogle Scholar
  13. Cichy KA, Caldas GV, Snapp SS, Blair MW (2009) QTL analysis of seed iron, zinc, and phosphorus levels in an Andean bean population. Crop Sci 49:1742–1750CrossRefGoogle Scholar
  14. Clemens S, Deinlein U, Ahmadi H, Höreth S, Uraguchi S (2013) Nicotianamine is a major player in plant Zn homeostasis. Biol Met 26:623–632Google Scholar
  15. Crespo-Hererra LA, Velu G, Singh RP (2016) QTL mapping reveals pleiotropic effect for grain iron and zinc concentrations in wheat. Ann Appl Biol. doi:10.1111/aab.12276 Google Scholar
  16. Distelfeld A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Budak H (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiol Plant 129:635–643CrossRefGoogle Scholar
  17. Genc Y, Huan CY (2007) A study of the role of root morphological traits in growth of barley in zinc-deficient soil. J Exp Bot 58:2775–2784CrossRefPubMedGoogle Scholar
  18. Genc Y, Verbyla AP, Torun AA, Cakmak I, Willsmore K, Wallwork H, McDonald GK (2009) Quantitative trait loci analysis of zinc efficiency and grain zinc concentration in wheat using whole genome average interval mapping. Plant Soil 314:49–66CrossRefGoogle Scholar
  19. Gomez-Becerra HF, Yazici A, Ozturk L, Budak H, Peleg Z, Morgounov A, Fahima T, Saranga Y, Cakmak I (2010a) Genetic variation and environmental stability of grain mineral nutrient concentrations in Triticum dicoccoides under five environments. Euphytica 171:39–52CrossRefGoogle Scholar
  20. Gomez-Becerra HF, Erdem YA, Tutus Y, Torun B, Ozturk L, Cakmak I (2010b) Grain concentrations of protein and mineral nutrients in a large collection of spelt wheat grown under different environments. J. Cereal Sci 52:342–349CrossRefGoogle Scholar
  21. Graham RD, Ascher JS, Hynes SC (1992) Selection of zinc efficient cereal genotypes for soils of low zinc status. Plant Soil 146:241–250CrossRefGoogle Scholar
  22. Graham RD, Senadhira D, Beebe S, Iglesias C, Monasterio I (1999) Breeding for micronutrient density in edible portions of staple food crops conventional approaches. Field Crop Res 60:57–80CrossRefGoogle Scholar
  23. Gupta PK, Langridge P, Mir RR (2010) Marker-assisted wheat breeding: present status and future possibilities. Mol Breed 26:145–161CrossRefGoogle Scholar
  24. Guzman C, Medina-Larque A, Velu G, Gonzalez H, Singh RP, Huerta J, Monasterio I, Pena J (2014) Use of wheat genetic resources to develop biofortified wheat with enhanced grain zinc and iron concentrations and desirable processing quality. J Cereal Sci 60:617–622CrossRefGoogle Scholar
  25. Hao Y, Chen Z, Wang Y, Bland D, Buck J, Brown-Guedira G, Johnson J (2011) Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat. Theor Applied Genet 123:1401–1411CrossRefGoogle Scholar
  26. Hao Y, Velu G, Pena RJ, Singh S, Singh RP (2014) Genetic loci associated with high grain zinc concentration and pleiotropic effect on kernel weight in wheat (Triticum aestivum L. Mol Breed 34:1893–1902CrossRefGoogle Scholar
  27. Holtz C, Brown KH (2004) Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 25:94–204Google Scholar
  28. Impa SM, Johnson-B, Sarah E (2012) Mitigating zinc deficiency and achieving high grain Zn in rice through integration of soil chemistry and plant physiology research. Plant Soil 361:3–41Google Scholar
  29. Joshi AK, Crossa I, Arun B, Chand R, Trethowan R, Vargas M, Ortiz-Monasterio I (2010) Genotype × environment interaction for zinc and iron concentration of wheat grain in eastern Gangetic plains of India. Field Crop Res 116:268–277CrossRefGoogle Scholar
  30. Joy EJM, Stein AJ, Young SD, Ander EL, Watts MJ, Broadley MR (2015) Zinc-enriched fertilizers as a potential public health intervention in Africa. Plant Soil 389:1–24CrossRefGoogle Scholar
  31. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428CrossRefGoogle Scholar
  32. Masuda H, Kobayashi T, Ishimaru Y, Takahashi M, Aung MS, Nakanshi H, Mor S, Nishizawa NK (2013) Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Front. Plant Sci 4. doi:10.3389/fpls.2013.00132
  33. Mori N, Ohta S, Chiba H, Takagi T, Niimi Y, Shinde V, Kajale MD, Osado T (2013) Rediscovery of Indian dwarf wheat (Triticum aestivum L. ssp. sphaerococcum (Perc.) MK.) an ancient crop of the Indian subcontinent. Genetic Res. Crop Evol 60:1771–1775CrossRefGoogle Scholar
  34. Nube M, Voortman RL (2011) Human Micronutrient Deficiencies: Linkages with Micronutrient Deficiencies in Soils, Crops and Animal Nutrition. In Combating Micronutrient Deficiencies: Food Based Approaches eds Thompson B, Amoroso L pp. 289–311. USA.Google Scholar
  35. Ortiz-Monasterio I, Graham RD (2000) Breeding for trace minerals in wheat. Food Nutr Bull 21:393–396Google Scholar
  36. Ozkan H, Brandolini A, Torun A, Altintas S, Eker S, Kilian B, Braun HJ, Salamini F, Cakmak I (2007) Natural variation and identification of microelements content in seeds of Einkorn Wheat (Triticum monococcum). In Proceedings of the 7th International Wheat Conference, 27 November–2 December 2005, Mar del Plata, Argentina pp 455–462.Google Scholar
  37. Paltridge NG, Milham PJ, Ortiz-Monasterio JI, Velu G, Yasmin Z, Palmer LJ, Guild GE, Stangoulis JCR (2012) Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat. Plant Soil 361:251–260CrossRefGoogle Scholar
  38. Peleg Z, Saranga Y, Yazici A, Fahima T, Ozturk L, Cakmak I (2008) Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes. Plant Soil 306:57–67CrossRefGoogle Scholar
  39. Peleg Z, Cakmak I, Ozturk L, Yazici A, JunY BH, Korol AB, Fahima T, Saranga Y (2009) Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat x wild emmer wheat RIL population. Theor App Gen 119:353–369CrossRefGoogle Scholar
  40. Pfeiffer WH, McClafferty B (2007) HarvestPlus: breeding crops for better nutrition. Crop Sci 47:S88–S105CrossRefGoogle Scholar
  41. Randhawa HS, Asif M, Pozniak C, Clarke JM, Graf RJ, Fox S, Humphreys DG, Knox R, Depauw R, Singh AK, Cuthbert R, Hucl P, Spaner D, Gupta P (2013) Application of molecular markers to wheat breeding in Canada. Plant Breed 132:458–471Google Scholar
  42. Rengel Z, Batten GD, Crowley DE (1999) Agronomic approaches for improving the micronutrient density in edible portions of field crops. Field Crop Res 60:27–40CrossRefGoogle Scholar
  43. Singh K, Chhuneja P, Tiwari VK, Rawat N, Neelam K, Aggarwal R, Malik S, Keller B, Dhaliwal HS (2010) Mapping of QTL for grain iron and zinc content in diploid A genome wheat and validation of these loci in U and S genomes. Pag Conference, San Diego, USA, InGoogle Scholar
  44. Srinivasa J, Arun B, Mishra VK, Singh GP, Velu G, Babu R, Vasistha NK, Joshi AK (2014) Zinc and iron concentration QTL mapped in a Triticum spelta × T. aestivum cross. Theor App Genet 127:1643–1651CrossRefGoogle Scholar
  45. Tiwari VK, Rawat N, Chhuneja P, Neelam K, Aggarwal R, Randhawa GS, Dhaliwal HS, Keller B, Singh K (2009) Mapping of quantitative trait loci for grain iron and zinc concentration in diploid a genome wheat. J Herpetol 100:771–776Google Scholar
  46. Tiwari C, Wallwork H, Arun B, Mishra VK, Velu G, Stangoulis JCR, Kumar U, Joshi AK (2016) Molecular mapping of quantitative trait loci for zinc, iron and protein content in the grains of hexaploid wheat. Euphytica 207:563–570CrossRefGoogle Scholar
  47. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301CrossRefPubMedPubMedCentralGoogle Scholar
  48. Velu G, Singh RP, Huerta-Espino J, Peña-Bautista RJ, Arun B, Mahendru-Singh A, Yaqub Mujahid M, Soh VS, Mavi GS, Crossa J, Alvarado G, Joshi AK, Pfeiffer WH (2012) Performance of biofortified spring wheat genotypes in target environments for grain zinc and iron concentrations. Field Crop Res 137:261–267CrossRefGoogle Scholar
  49. Velu G, Singh R, Arun B, Mishra VK, Tiwari C, Joshi A, Cherian B, Virk P, Pfeiffer WH (2015) Reaching out to farmers with high zinc wheat varieties through public-private partnerships – An experience from Eastern-Gangetic Plains of India. Adv Food Tech Nutr Sci 1:73–75CrossRefGoogle Scholar
  50. Velu G, Guzman C, Mondal S, Autrique JE, Huerta J, Singh RP (2016) Effect of drought and elevated temperature on grain zinc and iron concentrations in CIMMYT spring wheat. J Cereal Sci 69:182–186CrossRefGoogle Scholar
  51. Waines JG, Ehdaie B (2007) Domestication and crop physiology: roots of green-revolution wheat. Ann Bot 100:991–998CrossRefPubMedPubMedCentralGoogle Scholar
  52. Wang S, Basten CJ, Zeng ZB (2012) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC.Google Scholar
  53. Welch RM, Graham RD (2004) Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot 55:353–364CrossRefPubMedGoogle Scholar
  54. White PJ, Broadley MR (2009) Biofortification of crops with seven mineral elements often lacking in human diets -iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol 182:49–84CrossRefPubMedGoogle Scholar
  55. Xu YF, An DG, Liu DC, Zhang AM, Xu HX, Li B (2012) Molecular mapping of QTLs for grain zinc, iron and protein concentration of wheat across two environments. Field Crop Res 138:57–62CrossRefGoogle Scholar
  56. Yasmin Z, Paltridge R, Graham R, Huynh BL, Stangoulis J (2013) Measuring genotypic variation in wheat seed iron first requires stringent protocols to minimize soil iron contamination. Crop Sci 54:255–264CrossRefGoogle Scholar
  57. Zhao FJ, YH S, Dunham SJ, Rakszegi M, Bedo Z, McGrath SP, Shewry PR (2009) Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. J Cereal Sci 49:290–295CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Govindan Velu
    • 1
  • Yusuf Tutus
    • 2
  • Hugo F. Gomez-Becerra
    • 3
  • Yuanfeng Hao
    • 1
  • Lütfü Demir
    • 4
  • Rukiye Kara
    • 5
  • Leonardo A. Crespo-Herrera
    • 1
  • Sinasi Orhan
    • 4
  • Atilla Yazici
    • 2
  • Ravi P. Singh
    • 1
  • Ismail Cakmak
    • 2
  1. 1.International Maize and Wheat Improvement Center (CIMMYT)MexicoMexico
  2. 2.Faculty of Engineering and Natural SciencesSabanci UniversityIstanbulTurkey
  3. 3.Bayer Crop Science, Nebraska Research StationLincolnUSA
  4. 4.Maize Research InstituteSakaryaTurkey
  5. 5.East Mediterranean Transitional Zone Agricultural Research of InstituteKahramanmarasTurkey

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