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Low doses of exogenous methyl jasmonate applied simultaneously with toxic aluminum improve the antioxidant performance of Vaccinium corymbosum

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Abstract

Aims

The effect of different MeJA doses applied prior to or simultaneously with toxic Al on biochemical and physiological properties of Vaccinium corymbosum cultivars with contrasting Al resistance was studied.

Methods

Legacy (Al-resistant) and Bluegold (Al-sensitive) plants were treated with and without toxic Al under controlled conditions: a) without Al and MeJA, b) 100 μM Al, c) 100 μM Al + 5 μM MeJA, d) 100 μM Al + 10 μM MeJA and e) 100 μM Al + 50 μM MeJA. MeJA was applied to leaves 24 h prior to or simultaneously with Al in nutrient solution. After 48 h, Al-concentration, lipid peroxidation (LP), H2O2, antioxidant activity, total phenols, total flavonoids, phenolic compounds and superoxide dismutase activity (SOD) of plant organs were analyzed.

Results

Al-concentrations increased with Al-treatment in both cultivars, being Al, LP and H2O2 concentrations reduced with low simultaneous MeJA application. Higher MeJA doses induced more oxidative damage than the lowest. Legacy increased mainly non-enzymatic compounds, whereas Bluegold increased SOD activity to counteract Al3+.

Conclusions

Low MeJA doses applied simultaneously with Al3+ increased Al-resistance in Legacy by increasing phenolic compounds, while Bluegold reduced oxidative damage through increment of SOD activity, suggesting a diminution of its Al-sensitivity. Higher MeJA doses could be potentially toxic. Studies are needed to determine the molecular mechanisms involved in the protective MeJA effect against Al-toxicity.

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Acknowledgments

We are very grateful for FONDECYT Project N° 1120917 which supported this work and PhD fellowship N°21110919, both from the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) of the Government of Chile, as well as the 2013 DI 13-2017 and 2015 DI 15-2015 Projects from the Dirección de Investigación at the Universidad de La Frontera, Temuco, Chile. We would like to thank Graciela Muñoz Pozo for her valuable assistance in the laboratory, and Dr. Helen Lowry for revising the language of the manuscript. Finally, we wish to thank the Doctorado en Ciencias de Recursos Naturales Program and the Laboratorio de Fisiología y Bioquímica Vegetal at the Universidad de La Frontera, Temuco, Chile.

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Authors and Affiliations

  1. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, P.O. Box 54-D, Temuco, Chile

    Elizabeth M. Ulloa-Inostroza

  2. Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, P.O. Box 54-D, Temuco, Chile

    M. Alberdi & M. Reyes-Díaz

  3. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, P.O. Box 54-D, Temuco, Chile

    M. Alberdi, C. Meriño-Gergichevich & M. Reyes-Díaz

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  1. Elizabeth M. Ulloa-Inostroza
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  2. M. Alberdi
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  3. C. Meriño-Gergichevich
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  4. M. Reyes-Díaz
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Correspondence to M. Reyes-Díaz.

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Ulloa-Inostroza, E.M., Alberdi, M., Meriño-Gergichevich, C. et al. Low doses of exogenous methyl jasmonate applied simultaneously with toxic aluminum improve the antioxidant performance of Vaccinium corymbosum . Plant Soil 412, 81–96 (2017). https://doi.org/10.1007/s11104-016-2985-z

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