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Plant and Soil

, Volume 412, Issue 1–2, pp 35–42 | Cite as

Sample storage conditions alter colonisation structures of arbuscular mycorrhizal fungi and, particularly, fine root endophyte

  • Suzanne OrchardEmail author
  • R. J. Standish
  • D. Nicol
  • I. A. Dickie
  • M. H. Ryan
Regular Article

Abstract

Background and Aims

The structures of arbuscular mycorrhizal (AM) fungi (hyphae, arbuscules, vesicles, spores) are used to make inferences about fungal activity based on stored samples, yet the impact of storage method has not been quantified, despite known effects of temperature and host condition on AM fungal colonisation.

Methods

We measured how four storage treatments (cool or ambient conditions, with and without plant shoots attached, i.e. n = four treatment combinations) affected AM fungal colonisation of subterranean clover (Trifolium subterraneum L.) after 0, 2, 6 and 10 days of storage. Roots were assessed for colonisation of fine root endophyte and coarse AM fungi.

Results

For coarse AM fungi, total colonisation was unaffected, but arbuscules were reduced at Day 6 and increased again by Day 10, except Ambient-Minus-Shoots. There was a loss of vesicles in all treatments at Day 2, and an increase in spore number at Day 6 within Cool-Plus-Shoots. In contrast, for fine root endophyte, total colonisation was greatly reduced at Day 6 but increased again at Day 10, in all except the Cool-Plus-Shoots treatment.

Conclusions

Our data demonstrate that AM fungal activity is not suspended in commonly used plant storage conditions. Storage method and time impacted AM fungal colonisation, particularly for fine root endophyte. We recommend samples are processed within 2 days of harvest.

Keywords

Arbuscules Fine endophyte Glomus tenue Mycorrhizal structures Plant sample storage Spores Vesicles 

Notes

Acknowledgments

This research formed part of a project supported by an Australian Government Postgraduate Award, a Meat and Livestock Australia Postgraduate Scholarship and a Henry Schapper Postgraduate Research Scholarship, and we gratefully acknowledge this funding. We wish to thank two anonymous reviewers for their constructive comments on this manuscript.

References

  1. Abbott L (1982) Comparative anatomy of vesicular-arbuscular mycorrhizas formed on subterranean clover. Aust J Bot 30:485–499CrossRefGoogle Scholar
  2. Aguilar-Trigueros CA, Hempel S, Powell JR et al (2015) Branching out: towards a trait-based understanding of fungal ecology. Fungal Biol Rev 29:34–41CrossRefGoogle Scholar
  3. Alexander T, Meier R, Toth R, Weber HC (1988) Dynamics of arbuscule development and degeneration in mycorrhizas of Triticum aestivum L. and Avena sativa L. with reference to Zea mays L. New Phytol 110:363–70CrossRefGoogle Scholar
  4. Ba L, Ning J, Wang D et al (2012) The relationship between the diversity of arbuscular mycorrhizal fungi and grazing in a meadow steppe. Plant Soil 352:143–56CrossRefGoogle Scholar
  5. Braunberger PG, Abbott LK, Robson AD (1997) Early vesicular-arbuscular mycorrhizal colonisation in soil collected from an annual clover-based pasture in a Mediterranean environment: soil temperature and the timing of autumn rains. Aust J Agric Res 48:103–10CrossRefGoogle Scholar
  6. Brundrett M (2009) Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant Soil 320:37–77CrossRefGoogle Scholar
  7. Brundrett MC, Kendrick B (1988) The mycorrhizal status, root anatomy, and phenology of plants in a sugar maple forest. Can J Bot 66:1153–1173CrossRefGoogle Scholar
  8. Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N (1996) Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research, CanberraGoogle Scholar
  9. Crush JR (1974) Plant growth responses to vesicular-arbuscular mycorrhiza. VII. Growth and nodulation of some herbage legumes. New Phytol 73:743–752CrossRefGoogle Scholar
  10. Denison RF, Kiers ET (2011) Life histories of symbiotic rhizobia and mycorrhizal fungi. Curr Biol 21:R775–R85CrossRefPubMedGoogle Scholar
  11. Gianinazzi-Pearson V, Gianinazzi S (1983) The physiology of vesicular-arbuscular mycorrhizal roots. Plant Soil 71:197–209CrossRefGoogle Scholar
  12. Gianinazzi-Pearson V, Morandi D, Dexheimer J, Gianinazzi S (1981) Ultrastructural and ultracytochemical features of a Glomus tenuis mycorrhiza. New Phytol 88:633–639CrossRefGoogle Scholar
  13. Hall IR (1977) Species and mycorrhizal infections of New Zealand endogonaceae. Trans Br Mycol Soc 68:341–356CrossRefGoogle Scholar
  14. Hayman DS (1983) The physiology of vesicular–arbuscular endomycorrhizal symbiosis. Can J Bot 61:944–963CrossRefGoogle Scholar
  15. Ijdo M, Schtickzelle N, Cranenbrouck S, Declerck S (2010) Do arbuscular mycorrhizal fungi with contrasting life-history strategies differ in their responses to repeated defoliation? FEMS Microbiol Ecol 72:114–122CrossRefPubMedGoogle Scholar
  16. Klironomos JN, McCune J, Moutoglis P (2004) Species of arbuscular mycorrhizal fungi affect mycorrhizal responses to simulated herbivory. Appl Soil Ecol 26:133–141CrossRefGoogle Scholar
  17. McArthur WM, Bettenay E (1959) The soils and irrigation potential of the Pinjarra-Waroona area, Western Australia. CSIRO, Division of Soils, MelbourneGoogle Scholar
  18. McGee PA (1989) Variation in propagule numbers of vesicular-arbuscular mycorrhizal fungi in a semi-arid soil. Mycol Res 92:28–33CrossRefGoogle Scholar
  19. McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular—arbuscular mycorrhizal fungi. New Phytol 115:495–501CrossRefGoogle Scholar
  20. Mosse B (1973) Advances in the study of vesicular-arbuscular mycorrhiza. Annu Rev Phytopathol 11:171–196CrossRefGoogle Scholar
  21. Newsham KK, Upson R, Read DJ (2009) Mycorrhizas and dark septate root endophytes in polar regions. Fungal Ecol 2:10–20CrossRefGoogle Scholar
  22. Orchard S, Standish RJ, Nicol D, Gupta VV, Ryan MH (2016) The response of fine root endophyte (Glomus tenue) to waterlogging is dependent on host plant species and soil type. Plant Soil. doi: 10.1007/s11104-016-2804-6 Google Scholar
  23. Pawlowska TE, Douds DD Jr, Charvat I (1999) In vitro propagation and life cycle of the arbuscular mycorrhizal fungus Glomus etunicatum. Mycol Res 103:1549–1556CrossRefGoogle Scholar
  24. Pearson JN, Smith SE, Smith FA (1991) Effect of photon irradiance on the development and activity of VA mycorrhizal infection in Allium porrum. Mycol Res 95:741–746CrossRefGoogle Scholar
  25. Peay KG (2014) Back to the future: natural history and the way forward in modern fungal ecology. Fungal Ecol 12:4–9CrossRefGoogle Scholar
  26. Read DJ (1991) Mycorrhizas in ecosystems. Experientia 47:376–391CrossRefGoogle Scholar
  27. Read DJ, Haselwandter K (1981) Observations of the mycorrhizal status of some alpine plant communities. New Phytol 88:341–352CrossRefGoogle Scholar
  28. Saravesi K, Ruotsalainen AL, Cahill JF (2014) Contrasting impacts of defoliation on root colonization by arbuscular mycorrhizal and dark septate endophytic fungi of Medicago sativa. Mycorrhiza 24:239–245CrossRefPubMedGoogle Scholar
  29. Schussler A and Walker C (2010) The glomeromycota. A species list with new families and new genera. www.amf-phylogeny.com. Accessed 25 March 2015Google Scholar
  30. Shi P, Abbott LK, Banning NC, Zhao B (2012) Comparison of morphological and molecular genetic quantification of relative abundance of arbuscular mycorrhizal fungi within roots. Mycorrhiza 22:501–513CrossRefPubMedGoogle Scholar
  31. Shi G, Liu Y, Johnson N et al (2014) Interactive influence of light intensity and soil fertility on root-associated arbuscular mycorrhizal fungi. Plant Soil 378:173–188CrossRefGoogle Scholar
  32. Simon L, Lalonde M, Bruns TD (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol 58:291–295PubMedPubMedCentralGoogle Scholar
  33. Smith S, Gianinazzi-Pearson V (1990) Phosphate uptake and arbuscular activity in mycorrhizal Allium cepa L.: effects of photon irradiance and phosphate nutrition. Funct Plant Biol 17:177–188Google Scholar
  34. Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Elsevier, BurlingtonGoogle Scholar
  35. Staddon PL, Fitter AH (2001) The differential vitality of intraradical mycorrhizal structures and its implications. Soil Biol Biochem 33:129–132CrossRefGoogle Scholar
  36. Tester M, Smith FA, Smith SE (1985) Phosphate inflow into Trifolium subterraneum L.: effects of photon irradiance and mycorrhizal infection. Soil Biol Biochem 17:807–810CrossRefGoogle Scholar
  37. Thippayarugs S, Bansal M, Abbott LK (1999) Morphology and infectivity of fine endophyte in a Mediterranean environment. Mycol Res 103:1369–1379CrossRefGoogle Scholar
  38. Vierheilig H, Coughlan AP, Wyss U, Piche Y (1998) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl Environ Microbiol 64:5004–5007PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Suzanne Orchard
    • 1
    Email author
  • R. J. Standish
    • 2
  • D. Nicol
    • 1
    • 3
  • I. A. Dickie
    • 4
  • M. H. Ryan
    • 1
  1. 1.School of Plant Biology, and Institute of AgricultureThe University of Western AustraliaPerthAustralia
  2. 2.School of Veterinary & Life SciencesMurdoch UniversityMurdochAustralia
  3. 3.Department of Agriculture and FoodDryland Research InstituteMerredinAustralia
  4. 4.Bio-Protection Research CentreLincoln UniversityLincolnNew Zealand

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