Verticillium wilt is difficult to suppress, and causes severe yield losses in a broad range of crops. Five Serratia and five Paenibacillus endophytic isolates showing antagonistic properties against fungal pathogens were compared for their plant growth-promoting (PGP) potential under different plant growth conditions with the objective of evaluating the PGP of endophytic strains in different ad planta systems.
Preselected isolates were applied to the surface-sterilized seeds of oilseed rape and cauliflower using bio-priming. The isolates’ PGP effect and root colonization capacities were compared under gnotobiotic conditions. One strain from each genus was selected and tested for its PGP qualities in sterile and non-sterile soil.
Serratia treatment resulted in different levels of PGP, while Paenibacillus strains damaged roots under gnotobiotic conditions. P. polymyxa Sb3-1 did not have a significant effect on plant growth in non-sterile soil; however it did promote plant growth in the sterile soil. S. plymuthica 3RP8 and P. polymyxa Sb3-1 were selected for further testing of their biocontrol effect under field conditions.
The choice of growth environments in the investigation of plant-bacterium interaction is crucial. Non-sterile soil is suggested as the ideal medium for use in studying the PGP effect.
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The authors would like to thank Timothy Mark (Graz) for English revision and discussion. This project was funded by the European Union in frame of FP7-KBBE-2013-7-single-stage (BIOCOMES; No. 612713) and by the Austrian Research Promotion Agency (FFG; No. 836466). The authors gratefully acknowledge support from NAWI Graz. We thank Christin Zachow (Graz) for her help regarding experimental questions and Anastasia Bragina (Graz) for help regarding statistical issues.
Responsible Editor: Birgit Mitter.
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Rybakova, D., Schmuck, M., Wetzlinger, U. et al. Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions. Plant Soil 405, 65–79 (2016). https://doi.org/10.1007/s11104-015-2572-8
- Plant growth promotion
- Verticillium wilt