Endophytes-assisted biocontrol: novel insights in ecology and the mode of action of Paenibacillus
- 1.8k Downloads
Biological control is an environmentally sound and effective means of reducing pathogen-induced damage to agriculture using natural antagonists. Paenibacillus is a cosmopolitan and ubiquitously occurring bacterial genus with antagonistic activity against phytopathogens. Many species and strains with promising potential for plant growth promotion and biocontrol of pathogens have been identified since Paenibacillus was first described 20 years ago. Nevertheless, important questions regarding the colonization of plants, and the mode of action of Paenibacillus remain unanswered.
This review focuses on the occurrence of Paenibacillus in microbial metagenomes, the endophytic lifestyle of Paenibacillus, and the function of Paenibacillus-derived volatile organic compounds (VOCs) combining actual literature with our own results.
This review provides new insights into the endophytic lifestyle of Paenibacillus and discusses strain-specific and system-dependent growth promotion effects on plants. VOCs, in particular pyrazine derivatives emitted by Paenibacillus, showed high activity against other organisms. This suggests that VOCs play an important role in communication and interaction. Overall, Paenibacillus strains demonstrate promising potential not only for sustainable agriculture and biological control, but also as a source for novel bioactive volatiles.
KeywordsPGPR Plant growth promotion Endophyte Volatiles Metagenomics
The authors would like to thank Timothy Mark (Graz) for English revision and discussion. This work was supported by three grants to GB, one from the Austrian Science Fund (FWF-DACH Project I882), another one affiliated to ACIB, the Austrian Centre of Industrial Biotechnology (supported by the Federal Ministry of Economy, Family and Youth (BMWFJ), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol and ZIT—Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG) and the 3rd is an EU-funded project (BIOCOMES, No. 612713).
- Aswathy AJ, Jasim B, Jyothis M, Radhakrishnan EK (2013) Identification of two strains of Paenibacillus sp. as indole 3 acetic acid-producing rhizome-associated endophytic bacteria from Curcuma longa. 3. Biotech 3:219–224Google Scholar
- Bacon CW, Hinton DM (1997) Isolation and culture of endophytic bacteria and fungi. In: Knudsen GR, McInerney MJ, Stetzenbach LD, Walter MV (eds) Manual of environmental microbiology. ASM Press, Washington, pp 413–421Google Scholar
- Cernava T (2012) Identification of volatile organic compounds from plant-associated bacteria. Graz University of Technology, Master thesisGoogle Scholar
- Cotta SR, da Mota FF, Tupinambá G, Ishida K, Rozental S, Silva DO E, da Silva AJ, Bizzo HR, Alviano DS, Alviano CS, Seldin L (2012) Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. World J Microbiol Biotechnol 28:953–962PubMedCrossRefGoogle Scholar
- Govindasamy V, Senthilkumar M, Magheshwaran V, Kumar U, Bose P, Sharma V, Annapurna K (2011) Bacillus and Paenibacillus spp.: potential PGPR for sustainable agriculture. In: Maheshwari D (ed) Plant growth and health promoting bacteria; microbiology monographs 18. Springer, Berlin Heidelberg, pp 333–364Google Scholar
- Hardoim PR, van Overbeek LS, Berg G, Pirttilä AM, Compante S, Campisano A, Döring M, Sessitsch A (2015) The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews, under revisionGoogle Scholar
- Lehninger AL (1975) Biochemistry: the molecular basis of cell structure and function. Worth, New YorkGoogle Scholar
- Logares R, Sunagawa S, Salazar G, Cornejo‐Castillo FM, Ferrera I, Sarmento H, Hingamp P, Ogata H, de Vargas C, Lima-Mendez G, Raes J, Poulain J, Jaillon O, Wincker P, Kandels-Lewis S, Karsteni E, Acinas SG (2014) Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities. Environ Microbiol 16:2659–2671PubMedCrossRefGoogle Scholar
- Moissl-Eichinger C, Auerbach AK, Probst AJ, Mahnert A, Tom L, Piceno Y, Andersen GL, Venkateswaran K, Rettberg P, Barczyk S, Pukall R, Berg G (2015) Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments. Sci Rep 5:9156PubMedPubMedCentralCrossRefGoogle Scholar
- Pettersson B, Rippere KE, Yousten AA, Priest FG (1999) Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov. Int J Syst Bacteriol 49:531–540PubMedCrossRefGoogle Scholar
- Raza W, Yang W, Shen QR (2008) Paenibacillus polymyxa: antibiotics, hydrolytic enzymes and hazard assessment. J Plant Pathol 90:419–430Google Scholar
- Rybakova D, Schmuck M, Berg G (2015) Kill or cure? The interaction between endophytic Paenibacillus and Serratia spp. and the host plant is shaped by the environment; submittedGoogle Scholar
- Smith EF (1911) Bacteria in relation to plant diseases. Carnegie Institute, Washington, USAGoogle Scholar
- Timmusk S, Nevo E (2011) Plant root associated biofilms. In: Maheshwari DK (ed) Bacteria in 3 agrobiology. Plant nutrient management. Springer Verlag, Berlin 3:285–300Google Scholar
- Timmusk S, Abd El-Daim IA, Copolovici L, Tanilas T, Kännaste A, Behers L, Nevo E, Seisenbaeva G, Stenström E, Niinemets Ü (2014) Drought-tolerance of wheat improved by rhizosphere bacteria from harsh environments: enhanced biomass production and reduced emissions of stress volatiles. PLoS One 9, e96086PubMedPubMedCentralCrossRefGoogle Scholar
- Xie JB, Du Z, Bai L, Tian C, Zhang Y, Xie JY, Wang T, Liu X, Chen X, Cheng Q, Chen S, Li J (2014) Comparative genomic analysis of N2-fixing and non-N2-fixing Paenibacillus spp.: organization, evolution and expression of the nitrogen fixation genes. PLoS Genet 10, e1004231PubMedPubMedCentralCrossRefGoogle Scholar