Plant and Soil

, Volume 394, Issue 1–2, pp 35–43 | Cite as

Does high pH give a reliable assessment of the effect of alkaline soil on seed germination? A case study with Leymus chinensis (Poaceae)

Regular Article

Abstract

Background and aims

Alkaline soils, characterized by high pH, are representative of degraded regions throughout the world. Studying germination in relation to alkalinity can contribute to understanding how species cope with such conditions. Although the effects of pH have been widely studied, it is unknown whether germination response to pH gradients created with buffer solutions is representative of the conditions experienced in alkaline soils. Our aims were to (1) determine if high pH gives an accurate assessment of the effects of alkaline soils on germination, and (2) identify the inhibitory factors for germination in alkaline soils.

Methods

Using Leymus chinensis seeds, germination was tested over a gradient of pH solutions prepared using Tris (50 mM and 100 mM) and H2O buffers and eight germination media prepared from non-alkaline and alkaline soils with different pH and electrical conductivities (EC). Additionally, solutions of 10–100 mM NaCl, Na2SO4, Na2CO3 and NaHCO3 were used to determine the main ions inhibiting seed germination.

Results

H2O-buffered pH had no effect on seed germination, and seed germination was much lower at all pH levels in 50 mM Tris–HCl solutions (pH 7.0–10.35) than in the H2O control (pH 7.05). No seeds germinated in 100 mM Tris–HCl buffers irrespective of the pH. In alkaline germination media (pH 10.04–10.61), high germination was obtained only at low EC. The rank order of the inhibitory effect of salts was Na2CO3 > NaHCO3 > NaCl > Na2SO4.

Conclusions

Buffer solutions used to simulate alkaline environments did not provide a reliable indicator of the effects of alkaline soils on seed germination. High pH of alkaline soil had no negative effects, and results suggest that salt composition and concentration, especially CO32− and HCO3, are key inhibitors.

Keywords

Alkaline soil Buffer Electrical conductivity Soil pH Vegetation restoration 

References

  1. Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination. Elsevier/Academic Press, San Diego, pp 17–18Google Scholar
  2. Basto S, Dorca-Fornell C, Thompson K, Rees M (2013) Effect of pH buffer solutions on seed germination of Hypericum pulchrum, Campanula rotundifolia and Scabiosa columbaria. Seed Sci Technol 41:298–302CrossRefGoogle Scholar
  3. Chachalis D, Reddy KN (2000) Factors affecting Campsis radicans seed germination and seedling emergence. Weed Sci 48:212–216CrossRefGoogle Scholar
  4. Chauhan BS, Gill G, Preston C (2006) Influence of environmental factors on seed germination and seedling emergence of rigid ryegrass (Lolium rigidum). Weed Sci 54:1004–1012CrossRefGoogle Scholar
  5. Chejara VK, Kristiansen P, Whalley RDB, Sindel BM, Nadolny C (2008) Factors affecting germination of coolatai grass (Hyparrhenia hirta). Weed Sci 56:543–548CrossRefGoogle Scholar
  6. Chi CM, Wang ZC (2010) Characterizing salt-affected soils of songnen plain using saturated paste and 1:5 soil-to-water extraction methods. Arid Land Res Manage 24:1–11CrossRefGoogle Scholar
  7. Chi CM, Zhao CW, Sun XJ, Wang ZC (2012) Reclamation of saline-sodic soil properties and improvement of rice (Oryza sativa L.) growth and yield using desulfurized gypsum in the west of Songnen Plain, northeast China. Geoderma 187–188:24–30CrossRefGoogle Scholar
  8. Ding X, Tian C, Zhang S, Song J, Zhang F, Mi G, Feng G (2010) Effects of NO3-N on the growth and salinity tolerance of Tamarix laxa Willd. Plant Soil 331:57–67CrossRefGoogle Scholar
  9. Duan D, Liu X, Khan MA, Gul B (2004) Effects of salt and water stress on the germination of Chenopodium glaucum L., seed. Pak J Bot 36:793–800Google Scholar
  10. Ebrahimi E, Eslami SV (2012) Effect of environmental factors on seed germination and seedling emergence of invasive Ceratocarpus arenarius. Weed Res 52:50–59CrossRefGoogle Scholar
  11. Flowers TJ, Gracia A, Koyama M, Yeo AR (1997) Breeding for salt tolerance in crop plants. the role of molecular biology. Acta Physiol Plant 19:427–433CrossRefGoogle Scholar
  12. Foley ME, Chao WS (2008) Growth regulators and chemicals stimulate germination of leafy spurge (Euphorbia esula) seeds. Weed Sci 56:516–522CrossRefGoogle Scholar
  13. Gao ZW, Zhu H, Gao JC, Yang CW, Mu CS, Wang DL (2011) Germination responses of Alfalfa (Medicago sativa L.) seeds to various salt-alkaline mixed stress. Afr J Agric Res 6:3793–3803Google Scholar
  14. Guan B, Zhou D, Zhang H, Tian Y, Japhet W, Wang P (2009) Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature. J Arid Environ 73:135–138CrossRefGoogle Scholar
  15. Guerrero-Alves J, Pla-Sentís I, Camacho R (2002) A model to explain high values of pH in an alkali sodic soil. Sci Agrár 59:763–770CrossRefGoogle Scholar
  16. Gupta RK, Abrol IP (1990) Reclamation and management of alkali soils. Indian J Agric Sci 60:1–16Google Scholar
  17. Gupta I, Basu PK (1988) Role of pH on natural regeneration of Tephrosia candida, an endangered species of North Bengal. Environ Ecol 6:537–541Google Scholar
  18. Harris PJC (1981) Value of laboratory germination and viability tests in predicting field emergence of Urena lobata L. Field Crop Res 4:237–245CrossRefGoogle Scholar
  19. Henig-Sever N, Eshel A, Ne’eman G (1996) pH and osmotic potential of pine ash as post-fire germination inhibitors. Physiol Plant 96:71–76CrossRefGoogle Scholar
  20. Huang Z, Zhang X, Gutterman Y (2003) Influences of light, temperature, salinity and storage on seed germination of Haloxylon ammodendron. J Arid Environ 55:453–464CrossRefGoogle Scholar
  21. Khajeh-Hosseini M, Powell AA, Bingham IJ (2003) The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci Technol 31:715–725CrossRefGoogle Scholar
  22. Kitajima K, Fenner M (2000) Ecology of seedling regeneration. In: Fenner M (ed) Seeds: the ecology of regeneration in plant communities. CABI Publishing, Wallingford, pp 331–359CrossRefGoogle Scholar
  23. Koger C, Reddy KN, Poston DH (2004) Factors affecting seed germination, seedling emergence, and survival of texasweed (Caperonia palustris). Weed Sci 52:989–995CrossRefGoogle Scholar
  24. Kopittke PM, Menzies NW (2005) Effect of pH on Na induced Ca deficiency. Plant Soil 269:119–129CrossRefGoogle Scholar
  25. Li R, Shi F, Fukuda K (2010) Interactive effects of salt and alkali stresses on seed germination, germination recovery, and seedling growth of a halophyte Spartina alterniflora (Poaceae). S Afr J Bot 76:380–387CrossRefGoogle Scholar
  26. Ma HY, Liang ZW, Wu HT, Huang LH, Wang ZC (2010) Role of endogenous hormones, glumes, endosperm and temperature on germination of Leymus chinensis (Poaceae) seeds during development. J Plant Ecol 3:269–277CrossRefGoogle Scholar
  27. Mandić V, Krnjaja V, Tomić Z, Bijelić Z, Žujović M, Simić A, Prodanović S (2012) Genotype, seed age and pH impacts on germination of alfalfa. Rom Biotechnol Lett 17:7205–7211Google Scholar
  28. Mashhady AS, Rowell DL (1978) Soil alkalinity, equilibria and alkalinity development. J Soil Sci 29:65–75CrossRefGoogle Scholar
  29. Menzies NW, Fulton IM, Kopittke RA, Kopittke PM (2009) Fresh water leaching of alkaline bauxite residue after sea water neutralization. J Environ Qual 38:2050–2057CrossRefPubMedGoogle Scholar
  30. Nakamura I, Hossain MA (2009) Factors affecting the seed germination and seedling emergence of red flower ragleaf (Crassocephalum crepidioides). Weed Biol Manag 9:315–322CrossRefGoogle Scholar
  31. Norsworthy JK, Oliveira MJ (2005) Coffee senna (Cassia occidentalis) germination and emergence is affected by environmental factors and seeding depth. Weed Sci 53:657–662CrossRefGoogle Scholar
  32. Panta S, Flowers T, Lane P, Doyle R, Haros G, Shabala S (2014) Halophyte agriculture: success stories. Environ Exp Bot 107:71–83CrossRefGoogle Scholar
  33. Pérez-Fernández MA, Calvo-Magro E, Montanero-Fernández J, Ovola-Velasco JA (2006) Seed germination in response to chemicals: effect of nitrogen and pH in the media. J Environ Biol 27:13–20PubMedGoogle Scholar
  34. Piovan MJ, Zapperi GM, Pratolongo PD (2014) Seed germination of Atriplex undulata under saline and alkaline conditions. Seed Sci Technol 42:286–292CrossRefGoogle Scholar
  35. Redmann RE, Abouguendia ZM (1979) Germination and seedling growth on substrates with extreme pH-laboratory evaluation of buffers. J Appl Ecol 16:901–907CrossRefGoogle Scholar
  36. Rengasamy P (2010) Soil processes affecting crop production in salt-affected soils. Aust J Soil Res 37:613–620Google Scholar
  37. Roig T, Bäckman P, Olofsson G (1993) Ionization enthalpies of some common zwitterionic hydrogen-ion buffers (HEPES, PIPES, HEPPS and BES) for biological research. Acta Chem Scand 47:899–901CrossRefGoogle Scholar
  38. Shi DC, Wang DL (2005) Effects of various salt-alkaline mixed stresses on Aneurolepidium chinense (Trin.) Kitag. Plant Soil 271:15–26CrossRefGoogle Scholar
  39. Sosa L, LLanes A, Reinoso H, Reginato M, Luna V (2005) Osmotic and specific ion effects on the germination of Prosopis strombulifera. Ann Bot 96:261–267PubMedCentralCrossRefPubMedGoogle Scholar
  40. Stokes CA, MacDonald GE, Adams CR, Langeland KA, Miller DL (2011) Seed biology and ecology of natalgrass (Melinis repens). Weed Sci 59:527–532CrossRefGoogle Scholar
  41. Szabó A, Tóth T (2011) Relationship between soil properties and natural grassland vegetation on sodic soils. Ecol Quest 14:65–67CrossRefGoogle Scholar
  42. Tanji KK (1990) Nature and extent of agricultural salinity. In: Tanji KK (ed) Agricultural and salinity assessment and management. American Society of Civil Engineers, New York, pp 1–17Google Scholar
  43. Tavakkoli E, Fatehi F, Coventry S, Rengasamy P, McDonald GK (2011) Additive effects of Na+ and Cl ions on barley growth under salinity stress. J Exp Bot 62:2189–2203PubMedCentralCrossRefPubMedGoogle Scholar
  44. Tobe K, Li X, Omasa K (2002) Effects of sodium, magnesium and calcium salts on seed germination and radicle survival of a halophyte, Kalidium caspicum (Chenopodiaceae). Aust J Bot 50:163–169CrossRefGoogle Scholar
  45. Tobe K, Li X, Omasa K (2004) Effects of five different salts on seed germination and seedling growth of Haloxylon ammodendron (Chenopodiaceae). Seed Sci Res 14:345–353CrossRefGoogle Scholar
  46. Valkó O, Tóthmérész B, Kelemen A, Simon E, Migléc T, Lukác BA, Török P (2014) Environmental factors driving seed bank diversity in alkali grasslands. Agric Ecosyst Environ 182:80–87CrossRefGoogle Scholar
  47. Yu J, Wang Z, Meixner FX, Yang F, Wu H, Chen X (2010) Biogeochemical characterizations and reclamation strategies of saline sodic soil in northeastern China. Clean: Soil Air Water 38:1010–1016Google Scholar
  48. Zhou D, Xiao M (2010) Specific ion effects on the seed germination of sunflower. J Plant Nut 33:255–266CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Hongyuan Ma
    • 1
  • Haoyu Yang
    • 1
  • Xiaotao Lü
    • 2
  • Yuepeng Pan
    • 3
  • Haitao Wu
    • 1
  • Zhengwei Liang
    • 1
  • Mark K. J. Ooi
    • 4
  1. 1.Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchunChina
  2. 2.State Key Laboratory of Forest and Soil Ecology, Institute of Applied EcologyChinese Academy of SciencesShenyangChina
  3. 3.State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry (LAPC), Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina
  4. 4.Centre for Sustainable Ecosystem Solutions, School of Biological SciencesUniversity of WollongongNew South WalesAustralia

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