Plant and Soil

, Volume 392, Issue 1–2, pp 357–370 | Cite as

X-ray fluorescence microscopy of zinc localization in wheat grains biofortified through foliar zinc applications at different growth stages under field conditions

  • Babasola Ajiboye
  • Ismail Cakmak
  • David Paterson
  • Martin D. de Jonge
  • Daryl L. Howard
  • Samuel P. Stacey
  • Ayfer A. Torun
  • Nevzat Aydin
  • Michael J. McLaughlin
Regular Article

Abstract

Aim

Biofortification of wheat with zinc (Zn) through foliar Zn application has been proposed as an agronomic strategy to increase grain Zn concentration, which could serve as a nutritional intervention in regions with dietary Zn deficiency.

Methods

Bread wheat (Triticum aestivum L.) was biofortified through foliar Zn applications at different growth stages. The concentration of Zn and associated micronutrient in harvested whole grains was determined by ICP-OES. Synchrotron-based X-ray fluorescence microscopy (XFM) was then used to investigate the localization of Zn and associated micronutrients in cross sections of these grains.

Results

The concentration of Zn and other micronutrients (Mn, Fe, and Cu) was higher in grains treated with foliar Zn during grain-filling (early milk/dough) than those treated at stem elongation. The increase in Zn concentration of wheat grain with foliar application during grain-filling can be attributed to the intense localization of Zn in the aleurone layer, modified aleurone, crease tissue, vascular bundle, and endosperm cavity, and to a modest localization in endosperm, which is the most dominant grain tissue. These tissues and the Zn they contain are presumed to remain after milling and can potentially increase the Zn concentration in wheat flour.

Conclusions

By using XFM, it was shown that foliar Zn spray represents an important agronomic tool for a substantial Zn enrichment of different fractions of wheat grain, especially the endosperm. Further investigation of the chemical speciation of Zn in the endosperm is recommended to assess Zn bioavailability in harvested whole grain of wheat that has been biofortified through different timing of foliar Zn application.

Keywords

Biofortification Foliar application Wheat grain Zn localization Zn fertilizer X-ray fluorescence microscopy Aleurone layer Endosperm Endosperm cavity 

Abbreviations

XFM

X-ray fluorescence microscopy

ICP-OES

Inductively coupled plasma optical emission spectroscopy

LA-ICP-MS

Laser ablation inductively coupled plasma mass spectroscopy

SIMS

Secondary ion mass spectroscopy

PIXE

Photon induced X-ray emission

XANES

X-ray absorption near edge structure spectroscopy

Notes

Acknowledgments

This research was undertaken on the X-ray Fluorescence Microscopy (XFM) beamline at the Australian Synchrotron, Victoria Australia. The sectioning of the grains with the vibratome was carried out at the Australian Centre for Plant Functional Genomics (ACPFG), which was facilitated by Dr Gwen Mayo. Ms Casey Doolette helped in preparing the transverse sections. This study was partially supported by the HarvestPlus program (www.harvestplus.org). Financial support by The Mosaic Co to the Fertilizer Technology Research Centre is duly acknowledged. The authors will like to thank Enzo Lombi for his useful comments during the draft manuscript review.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Babasola Ajiboye
    • 1
    • 7
  • Ismail Cakmak
    • 2
  • David Paterson
    • 3
  • Martin D. de Jonge
    • 3
  • Daryl L. Howard
    • 3
  • Samuel P. Stacey
    • 1
  • Ayfer A. Torun
    • 4
  • Nevzat Aydin
    • 5
  • Michael J. McLaughlin
    • 1
    • 6
  1. 1.Fertilizer Technology Research Centre, School of Agriculture, Food and Wine, Waite Research InstituteThe University of AdelaideAdelaideAustralia
  2. 2.Faculty of Engineering and Natural SciencesSabanci UniversityIstanbulTurkey
  3. 3.Australian SynchrotronClaytonAustralia
  4. 4.Faculty of Agriculture, Department of Soil ScienceCukurova UniversityAdanaTurkey
  5. 5.Karamanoglu Mehmetbey UniversityKaramanTurkey
  6. 6.Sustainable Agriculture Flagship, CSIRO Land and WaterUrrbraeAustralia
  7. 7.Baba Agrotech IncCalgaryCanada

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