Abstract
Aims
The objectives of this study were to compare techniques for measuring biological nitrogen fixation (BNF) and to assess how fertiliser N input affects the balance between BNF and sustainable herbage production on perennial ryegrass (Lolium perenne L.)/white clover (Trifolium repens L.) grassland.
Methods
Biological N fixation and herbage production by white clover based grassland was measured in 2011 and 2012 under four nominal annual fertiliser N inputs: 0 (0N), 86 (86N), 140 (140N) and 280 kg ha−1 (280N). Biological N fixation was measured using the 15N isotope dilution and 15N natural abundance techniques under all fertiliser N inputs and also using the nitrogen difference technique under 0N.
Results
The two 15N techniques produced similar annual estimates of above-ground BNF across the range of fertiliser N inputs. Fertiliser N input resulted in higher herbage dry matter yield, but reduced annual BNF which averaged 80, 64, 66 and 47 kg ha−1 on 0N, 86N, 140N and 280N, respectively, across both techniques and years.
Conclusions
The two 15N techniques were comparable in estimating BNF. Intermediate fertiliser N inputs achieved a balance between minimising detrimental impact on BNF and potential N loss while producing more herbage DM yield than clover swards receiving no fertiliser N.
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Acknowledgments
This work was funded by the EU Framework Programme 7 project Legume Futures. The author thanks Laura Lopez del Egido and the Stable Isotope Facility at the James Hutton Institute for assistance with the B s -value analyses and Kevin McNamara, internship students and the staff of Solohead Research Farm for assistance with field sampling.
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Table S.1
Shoot δ15N values of perennial ryegrass and a combination of weed species in the June 2012 harvest and their effect on the proportion of N derived from the atmosphere (%Ndfa) in clover when used as reference plants using the 15N natural abundance technique on treatments receiving annual fertiliser N inputs of 0 (0N), 86 (86N), 140 (140N) and 280 (280N) kg ha−1. (DOC 57 kb)
Fig S.1
Proportion of nitrogen derived from the atmosphere (%Ndfa) in white clover estimated by 15N isotope dilution (ID) data (white circle), 15N natural abundance data (black box) and 15N ID data using a B s -value (grey triangle) on the final three harvest dates and a supplementary harvest in March 2013 (mm–yy) on treatments receiving annual fertiliser N input of (a) 0, (B) 86, (c) 140 and (d) 280 kg ha−1. Error bars represents the interaction S.E.M of harvest date × technique. (GIF 3 kb)
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Burchill, W., James, E.K., Li, D. et al. Comparisons of biological nitrogen fixation in association with white clover (Trifolium repens L.) under four fertiliser nitrogen inputs as measured using two 15N techniques. Plant Soil 385, 287–302 (2014). https://doi.org/10.1007/s11104-014-2199-1
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DOI: https://doi.org/10.1007/s11104-014-2199-1